罗氏LightCycler Nano实时荧光定量PCR仪

tel: 400-6699-117 转 1000

罗氏诊断PCR仪, 小型，32 孔qPCR，32支持SYBR Green染料法，水解探针法，HRM检测;内置经典的绝对定量，相对定量和终点法......

参数指标

性能参数

激发装置白光 LED 全波长，高光强，超长使用寿命

检测装置 CCD 相机所有样本同时检测，快速精准

光学系统光纤导光避免光信号的损失，业内独有

激发检测波长 470/514 (SYBR, FAM, HRM) 533/572 (VIC, Hex, Yellow555) 577/620 (Red610, ROX, ) 645/697 (Cy5) 多重荧光 4重荧光, 带自动校正与颜色补偿

热循环系统 Peltier半导体元件, 96孔温控模块

升温速率 5℃/分钟

降温速率 4℃/分钟

温度均一性 ±0.2℃, STD < 0.1℃

温度准确性 ±0.2℃

温控范围 37- 98℃

梯度PCR功能有

梯度PCR温控范围 37 - 98℃

zei大梯度温控跨度 20℃ Cq 均一性 ±0.4, STD < 0.2, (区分2倍浓度差)

线性范围 10 个数量级

耗材 LightCycler 480 - 96孔板（白色，透明） LightCycler 八联管（白色）

分析模式绝对定量，相对定量，熔解曲线终点法基因分型，HRM* (v1.1 免费升级)，阴阳判定*(v1.1 免费升级)

Topic	模板	检测形式	试剂
动态范围定量（2倍稀释）	plasmid DNA	Universal ProbeLibrary probe	FastStart Essential DNA Probes Master
动态范围定量（10倍稀释）	plasmid DNA	Universal ProbeLibrary probe	FastStart Essential DNA Probes Master
Cq值的重复性	human genomic DNA	hydrolysis probe	FastStart Essential DNA Probes Master
终点法基因分型	DNA	hydrolysis probe	FastStart Essential DNA Probes Master
高分辨溶解曲线	DNA	ResoLight dye	LightCycler^® 480 High Resolution Melting Master
绝对定量	DNA	SYBR Green I	FastStart Essential DNA Green Master
均相Tm值	human genomic DNA	SYBR Green I	FastStart Essential DNA Green Master

　　广动力范围PCR

　　1动态范围定量(2倍稀释)

　　2动态范围定量(2倍稀释)

　　3 Cq值的重复性

　　4终点法基因分型

　　5高分辨溶解曲线

　　6绝对定量

　　7均相Tm值

System specifications:

Dimensions	W 9.45 in x D 10.63 in x H 9.06 in (W 24 cm x D 27 cm x H 23 cm)
Weight	15.43 lbs (7 kg)
Operating noise	<40 dB(A)
Electrical	Voltage: 100 - 240 V (+ 10%) Frequency: 50 - 60 Hz (+ 10%) Power: 170 W
Number of reactions	32
Reaction Vessels	8-tube strips (LightCycler® 8-Tube Strips, clear)
Reaction volume	10 - 100 µl (20 µl recommended)
Temperature cycling system	Peltier-based heating and cooling from +40 to+99°C Heating rate: 5°C/s Cooling Rate: 4°C/s
Temperature uniformity	+/- 0.1°C
Temperature accuracy	+/- 0.25°C
Run Time	<50 min
Fluorescence Excitation	500 nm nm (blue LED)
Fluorescence Detection	510 to 750 nm (CMOS camera)
Supported Assays Formats	Intercalating dyes (e.g., SYBR Green I), Hydrolysis Probes
Factory-Calibrated Dyes (at shipment)	SYBR Green I / FAM / ResoLight; VIC / HEX / Yellow555; LC RED 610 / Texas Red; Cy5
Multiplexing Capabilities	Dual color assays
Applications	Relative and Absolute Quantification Analysis Melting Peak Analysis Endpoint Genotyping Analysis High Resolution Melting (HRM)
Sensitivity	2-fold discrimination 9-log dynamic range Single copy detection
Connection options	LAN Direct connection to computer (RJ45) PC-free (USB stick/flash drive controlled)