SpectraMax M2/M2e 多功能读板机
Molecular Devices 生产的 SpectraMax M2/M2e 微孔板测读仪具有多种测读模式、顶部及底部测读 ( 仅限于 M2e )、双模式比色皿插槽、双光栅光路设计、“PathCheck”技术及微孔容量传感器。测读模式包括光吸收模式 ( UV/VIS 吸收 ) 和荧光强度模式(FI)。终点法、动力学、全波长扫描、单孔扫描和“PathCheck”传感器，上述特点使 M2 可进行各种均相和异相的微孔板检测。SpectraMax M2/M2e 可将非常低通量的检测轻松的优化转化为中高通量的分析，为您提供更快速准确的检测，并且节省大量的反应试剂。只需在软件上进行简单的操作就可以进行光吸收模式和荧光强度模式的选择。
主要特点
1,光栅式光谱发生器，连续波长200-1000nm，无需额外购买滤光片。
2,可检测吸光度（Abs)和荧光强度(FI)。吸收度波长范围达200－1000nm, 荧光波长范围250－850nm, 步进波长1nm。
3,可读取6、12、24、48、96和384孔微孔板。提供终点检测、动力学、单孔扫描和光谱扫描四种测读模式。
4,内置zlPathCheck探头，可把微孔板光径转化为1cm比色皿光径，还可校正移液器。
5,用各种酶标板，如平底板、圆底板、锥形底板等都能得到准确的结果。
6,利用先进的恒温虚拟盖技术，能精确控制样品舱内的温度。
7,具有功能强大的数据分析软件SoftMax Pro。
双光栅光路设计
有了 SpectraMax M2/M2e，就可以告别昂贵的滤光片，独特的双光栅光路设计可优化激发和发射波长。只需要简单的鼠标点击就可以对不同的实验方法和荧光试剂进行优化。
zlPathCheck 传感器
PathCheck 传感器是一款测量微孔板中样品高度 ( 光径 ) 的zl技术。使用SoftMax Pro 专业分析软件时，它能自动把微孔板中的样品高度转化为比色杯中的1 cm 光径，就如同使用 96 或 384 个比色皿收集数据一般。PathCheck 让你无需绘制标准曲线，对于已知消光系数的化合物，浓度可以直接根据吸光度值获得。
应用
DNA/RNA 蛋白定量和纯度
PicoGreen/NanoOrange/Bradford 实验
ELISAs/酶动力学 (i.e.,Km,Ki,etc.)
药物分解实验
细胞活性/毒性分析
Caspase-3 和蛋白酶实验
CatchPoint cAMP 实验
超微量检测
强大的数据分析功能
SoftMax Pro 提供数据获取，分析和管理功能，可进行多次微孔板的检测进行对比分析，允许用户自定义计算。它拥有完整的分析功能，无须把数据导出后分析。
软件和设备验证包
SpectraTest ABS1 光吸收和 SpectraTestFL1 荧光验证包可验证多种光路参数。SoftMax Pro 软件验证包和 IQ/OQ 验证协议提供符合 FDA 21 CFR Part 11 规格验证工具。
自定义数据采集及分析 | 光学验证板 | 超微量检测方案 |
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使用 SoftMax Pro 软件时，可以选择终点法、动力学、光谱扫描或单孔扫描模式；可实时查看动态变化及全波长扫描；能把来自多块微孔板、比色皿、曲线图、分析图表和注释中的数据收集存储在同一个数据文件中。 | SpectraTest 光吸收及荧光验证板可对SpectraMaxM2 读板机的光学性能进行自动校验，提供了一整套可追踪的方案。 | SpectraDrop 超微量板可以检测 24 或 64 孔板中低至 2 μL 的样品。 |
堆板机、机械臂一体化整合
SpectraMax M2/M2e 可以在几分钟内整合到 Molecular Devices 的StakMax 堆板机上。为了实现更高程度的自动化，自动化供应商合作伙伴已经把我们的酶标仪系统和所有主要合作伙伴的机械臂进行了整合。
高度满足客户的超微量检测需求
Molecular Devices 推出的 SpectraDrop超微量检测板，为超微量检测领域提供了目前市场上高通量解决方案。灵活革新性的特征设计极大缩短了样品准备时间，同时提高了实验室针对DNA、RNA 和蛋白质研究的效率。SpectraDrop 超微量检测板能够保证超微定量检测的超高一致性和重复性，同时还可以与 MD 的StakMax 堆板机系统无缝整合，大大增强了检测的能力与速度。
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Ameliorative effects of egg white hydrolysate on recognition memory impairments associated with chronic exposure to low mercury concentration
DA Rizzetti, CDC Altermann, CS Martinez… - Neurochemistry …, 2016 - Elsevier
... The formation of the oxidised fluorescent derivative (DCF) was measured by DCF fluorescence
intensity recorded at 520 nm (488 nm excitation) (Spectramax M5 Microplate/Cuvette Reader,
Molecular Devices, Pennsylvania, USA) for 60 min at 15 min intervals after the addition ...
Aluminum Exposure at Human Dietary Levels for 60 Days Reaches a Threshold Sufficient to Promote Memory Impairment in Rats
CS Martinez, CDC Alterman, FM Peçanha… - Neurotoxicity …, 2016 - Springer
... The DCF fluorescence intensity emission was recorded at 520 nm (with 480 nm excitation)
(SpectraMax M5 Molecular Devices, CA, USA) for 60 min at 15 min intervals. ... The color reaction
was measured at 532 nm against blanks (SpectraMax M5 Molecular Devices, CA, USA). ...
Quality decline and oxidative damage in sperm of freshwater crab Sinopotamon henanense exposed to lead
N Li, YH Hou, WX Jing, HU Dahms, L Wang - … and environmental safety, 2016 - Elsevier
... ROS was measured using a microplate reader Spectramax M5 (Molecular Devices
Corporation, California, USA) at an excitation of 485 nm and an emission of 525 nm. ... The
T-AOC was measured at 520 nm using a microplate reader Spectramax M5. ...
Cited by 1 Related articles All 3 versions Cite Save
A Two-photon Near Infrared (NIR-NIR) Fluorescent Turn-on Probe Towards Cysteine (Cys) and its Imaging Applications
J Wang, B Li, W Zhao, X Zhang, X Luo, M Corkins… - ACS …, 2016 - ACS Publications
... 10 mM PBS solution (pH = 7.4) was prepared as the buffer solution. UV−vis spectra were acquired
on a molecular devices Spectramax M5 spectrometer. Fluorescence spectra were measured
by RF-5301PC and Spectramax M5 spec- trometer. Synthesis of Probe TP-NIR. ...
Cited by 1 Related articles Cite Save
[HTML] In-Vitro dual inhibition of protein glycation, and oxidation by some Arabian plants
MA Siddiqui, S Rasheed… - BMC …, 2016 - bmccomplementalternmed. …
... It was then incubated at 37 °C for 9 days (under sterile conditions). After incubation, each sample
was examined for the development of fluorescence (λex 330 nm and λem 420 nm), against blank
on a microtitre plate reader (SpectraMax M5, Molecular Devices, CA, USA) [17]. ...
Advanced oxidation protein products induce catabolic effect through oxidant-dependent activation of NF-κ B pathway in human chondrocyte
W Ye, Z Zhong, S Zhu, S Zheng, J Xiao, S Song… - International …, 2016 - Elsevier
... The absorbance at wavelength of 570 nm was measured by a SpectraMax M5 system
(Molecular Devices, Califomia, USA). ... The absorbance was measured at λ = 525 nm on
a SpectraMax M5 system (Molecular Devices, Califomia, USA). 2.5. ...
Topical Minocycline Effectively Decontaminates and Reduces Inflammation in Infected Porcine Wounds
LT Daly, DM Tsai, M Singh, K Nuutila… - Plastic and …, 2016 - journals.lww.com
Background: Wound infection can impair postoperative
healing. Topical antibiotics have potential to.
MWCNTs induce ROS generation, ERK phosphorylation, and SOD-2 expression in human mesothelial cells
M Yu, R Chen, Z Jia, J Chen, J Lou… - International journal of …, 2016 - ijt.sagepub.com
... cells were treated with different concentrations (0, 10, 20, 40, and 80 µg/mL) of MWCNTs in a
total volume of 100 µL/well for 24 hours, and then 100 µL Cell Titer-Glo reagent was added into
each well, and luminescence was recorded with SpectraMax M5 multimode microplate ...
Cited by 2 Related articles All 4 versions Cite Save
[HTML] Delivery of a survivin promoter-driven antisense survivin-expressing plasmid DNA as a cancer therapeutic: a proof-of-concept study
KY Lin, SM Cheng, SL Tsai, JY Tsai, CH Lin… - OncoTargets and …, 2016 - ncbi.nlm.nih.gov
... Promoter activity was quantified by measuring the absorbance of the solution at
405 nm wavelength using the SpectraMax ® M5 microplate reader (Molecular
Devices LLC, Sunnyvale, CA, USA). Construction and production ...
Related articles All 4 versions Cite Save
Magnolia officinalis (Hou Po) bark extract stimulates the Nrf2-pathway in hepatocytes and protects against oxidative stress
A Rajgopal, SR Missler, JD Scholten - Journal of Ethnopharmacology, 2016 - Elsevier
... temperature. D-luciferin (100 µL) was added and light emission read immediately on
a SpectraMax M5 spectrophotometer (Molecular Devices, Sunnyvale, CA). ... signal.
Luminescence was read on a SpectraMax M5 spectrophotometer. ...
* 截至 2016 年 12 月 6 日。来源：Google Scholar。搜索结果包括 SpectraMax M 全系列（M、M2、M2e、M3、M4、M5、M5e）
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