X
您需要的产品资料:
选型配置
产品样品
使用说明
应用方案
公司录用
其他
个人信息:
提交

您好,欢迎您查看分析测试百科网,请问有什么帮助您的?

稍后再说 立即咨询
您现在所在的位置:首页 >> 仪器导购 >> 其他包装业专用>> MCL单分子成像Nano-Cyte®

MCL单分子成像Nano-Cyte®

tel: 400-6699-117 3355

MCL其他包装业专用, 本产品操作简单,使用便捷。......

在线客服
技术特点
【技术特点】-- MCL单分子成像Nano-Cyte®




Nano-Cyte Logo

3D stability for imaging

US Patent Number 9,019,363

  What is Nano-Cyte®?


Features3D stabilization up to 3 nanometersActive positional control over daysCorrects for temperature gradients and driftSimultaneous image acquisition and stabilizationParticle localization analysisParticle position renderingThe Nano-Cyte® single molecule imaging system eliminates the microscope drift that limits advanced fluorescence imaging methods. With Nano-Cyte® you no longer need to be concerned with temperature gradients, sample drift, and microscope drift. Unprecedented stability in the nanometer regime allows long term experiments as never before.

The Nano-Cyte® works by using the image of fluorescent fiduciary references, sparsely distributed within the sample, to localize these emitters in all three dimensions. The Nano-Cyte® uses this 3D localization information to provide active position adjustments to the sample, thus eliminating drift in the experiment.

Nano-Cyte® is the complete stabilization and image acquisition instrument for advanced fluorescence microscopy. Our integrated approach to 3D stabilization yields image stability up to 3nm in X,Y and Z axes. Nano-Cyte® has proven stability over days and is a unique offering that promises to revolutionize advanced microscopy methods.


  Hardware



The Nano-Cyte® is comprised of a high performance three axis nanopositioning system coupled with a two axis motorized micropositioning stage. These precision motion capabilities enable the active positional control and particle tracking features of the Nano-Cyte®. The nanopositioner is a flexure guided piezoactuated design with integrated PicoQ® sensors for absolute position sensing and nanometer precision under closed loop control. The micropositioning stage enables the user to have a large range of travel for surveying samples prior to engaging the active stabilization. All motion devices are controlled by the Nano-Cyte® controller via USB 2.0 interface.

The Nano-Cyte® hardware is compatible with Mad City Labs RM21™ open microscopy platform and most models of inverted optical microscopes.


  Method


Video: Nano-Cyte®: 3D Image Stabilization SystemThe Nano-Cyte® feedback control system. Stabilization is based on the imaging pathway.


  Software


The native Nano-Cyte® software performs 6 important functions:

  • Stabilization

  • Image acquisition

  • Device control

  • Particle localization analysis

  • Rendering of particle position

  • Tracking over multiple fields-of-view (FOV)


The Nano-Cyte® 3D stabilization occurs simultaneously with image acquistiion and incorporates reference selection, reference localization, and calibration statistics. Acquired images are saved in TIFF format and can be exported to ImageJ and other 3rd party software for post-acquisition processing.

Nano-Cyte® device control ensures precision motion control and the ability to incorporate a variety of external user devices such as EMCCD cameras, shutters and light sources. Compatibility with LabVIEW™ and μManager facilitates even greater user device control and flexibility.

Post-acquisition features of Nano-Cyte® enable the localization of particles within an image and the three dimensional rendering of particle positions.

Nano-Cyte® is compatible with LabVIEW™, μManager, ImageJVI and rapidSTORM. In addition, Nano-Cyte® has an exportable DLL to allow wider functionality with 3rd party software platforms.



  Stability Data


Nanometer Stability over Days


Stability data measured over 44 hours for the Z-axis. The red line indicates the measured drift. The blue line indicates the stabilized position when using Nano-Cyte®. Similar results were observed simultaneously for the X and Y-axis. These data below demonstrate the efficacy of the Nano-Cyte® under non-monotonic drift conditions.

 -->  

Effective Stability Using Nano-Cyte®


The Nano-Cyte® data below shows position changes performed on the z-axis over a period 1 hour. Similar results were observed simultaneously for the X and Y-axis. These data demonstrate that the Nano-Cyte® effective stability is 3nm.

 

  Nano-Cyte® Specifications


Stability (X, Y, Z-Axis)3 nmDrift rate compensation (typical)10 nm/secStabilization rate (typical)1 frame/secMaximum data acquisition rateCamera dependentNanopositioning range of motion200 μm × 200 μm × 200 μm      Position noise (total)0.4 nm      Step size3 nmMicropositioning range of motion25 mm x 25 mm      Encoder resolution20 nm      Minimum step size95 nmControllerNano-Cyte®CommunicationUSB 2.0TTL outputs4 channelsOutput image formatTIFFSoftwareNano-Cyte®Software compatibilityLabVIEWμManagerImageJ & ImageJVIExportable DLLRapidSTORMMicroscope compatibilityNikon Ti, TE SeriesZeiss Axio SeriesOlympus IX SeriesRM21™Supported EMCCD Camera typesAndorPhotometricsHamamatsuPower supply90-260 VAC (50/60Hz)Operating systemWindows Vista/7/8





Additional Information

Nano-Cyte® Brochure
Nano-Cyte brochure

Nano-Cyte® Video
Technical notes about sample preparation and applications are available by request.



Related Products

  • Nano-Cyte® Video

  • RM21™ Microscope Platform

  • C-Focus™

  • Nano-LPS Series

  • MicroStage Series

  • Nanopositioning Accessories

 


【技术特点对用户带来的好处】-- MCL单分子成像Nano-Cyte®


【典型应用举例】-- MCL单分子成像Nano-Cyte®


厂家资料

地址:北京市海淀区上地十街1号辉煌国际中心1号楼1006室

电话:400-6699-117 转3355

经销商

售后服务

我会维修/培训/做方法

如果您是一名工程师或者专业维修科学 仪器的服务商,都可参与登记,我们的平台 会为您的服务精确的定位并展示。

1该产品的品牌知名度如何?
2你对该产品的使用感受如何?
3该产品的性价比如何?
4该产品的售务如何?
查看
在线咨询

在线咨询时间:

周一至周五

早9:00 - 晚17:30

若您在周六周日咨询,请直接留言您所咨询的产品名称+联系人+电话