StepOne实时荧光定量PCR仪

StepOne™ 实时荧光定量PCR系统

即插即用，配备有易于使用的软件，适合初学者及有经验的实时荧光定量PCR仪用户使用

► 先进软件和仪器可运行广范围的各种基因组 Assay
► 灵敏、长寿命的三色光学LED记录系统，预先校准，可与 FAM™/SYBR Green、VIC®/JOE™ 和 ROX™ 染料配合使用
► 直观且稳定的软件，适合初学者或是经验丰富的用户使用
► 简单且灵活的仪器设置和使用
► 体积极小，适用于各种实验室环境
► LCD触摸屏和USB驱动使配置更加灵活，并可实现无PC操作

StepOne™ 实时荧光定量 PCR 系统是一款 48 孔低通量实时荧光定量PCR 仪，无论是初学者或是经验丰富的用户均适合使用。StepOne™ 实时荧光定量 PCR 系统配合直观的数据分析和仪器操控软件可进行各种反应设置，收到之后即可使用。StepOne™ 实时荧光定量 PCR 系统采用基于 LED 的稳定的三色光学系统，可提供精确的实时荧光定量 PCR 结果，适用于各种基因组研究应用领域。

StepOne™ 软件
StepOne™ 实时荧光定量 PCR 系统自带有 StepOne™ 软件，可在 Windows® XP、Windows Vista® 和 Windows® 7 操作系统上运行，具有仪器控制、数据采集和数据分析能力。zei新版本包括：
能够采集熔解曲线数据，可用于高分辨率熔解 (HRM) 实验
► 可选择以符合 MIQE 标准的实时荧光定量 PCR 数据标记语言 (RDML)格式输出数据
► 实验设计向导，帮助您设计和设置实验
► 远程监控，通过远程实时查看扩增情况，并在运行完成时通过电子邮件通知

此外，StepOne™ 实时荧光定量 PCR 系统自带的先进软件现包括功能强大的基因表达研究软件包。该软件包可提高基因表达分析的灵活性和准确性。

支持多种应用
应用领域包括基因表达分析、SNP 基因分型、microRNA 表达、易位分析、基因检测和病毒载量分析。为方便上述应用领域，Life Technologies提供了预先配制、已经过质量测试的即用型 TaqMan® Assay，与 StepOne™系统配合使用，帮助您节省分析优化的精力。

将您的 StepOne™ 系统升级至 StepOnePlus™ 系统
StepOnePlus™ 实时荧光定量 PCR 系统是 StepOne™ 系统的简单升级解决方案。您可以立即从三色、48 孔升级成四色、96 孔。在升级过程中，我们可提供 StepOnePlus™ 仪器供您暂时使用，这样您可以在仪器到货前继续您的研究。

StepOne™ 系统包括：
► 预校准的仪器
► RNA酶P仪器验证反应板
► 仪器操作和分析软件，以及 Primer Express® 引物和探针设计软件
► 包括入门指南在内的丰富的文件集，适用于各种主要的实时荧光定量 PCR 应用领域

Features of the The StepOne™ Real-Time PCR System include:

　　• Advanced software and instrumentation for performing a wide array of genomic assays.

　　• Sensitive 3-color optical LED recording system

　　• Intuitive and robust software perfect for both first-time and advanced users

　　• Simple and flexible instrument set-up and usage

　　StepOne™ Software

　　The StepOne™ Software included with the StepOne™ Real-Time PCR System runs on Windows® XP, Window Vista®, and Windows® 7 operating systems and provides instrument control, data collection, and data analysis capabilities. This latest version includes capabilities to collect melt curve data for High Resolution Melt (HRM) applications and the option to export in Real-Time PCR Data Markup Language (RDML) for compatibility with MIQE guidelines.

　　Rapid and Simple Assay Set-Up

　　This remarkably simple Real-Time PCR system is designed with an LCD touchscreen and a user-friendly interface that brings the power of genetic studies to researchers new to Real-Time PCR (Fig 1). The intuitive software and protocol wizards help guide new users through their Real-Time PCR experiments. The StepOne™ Real-Time PCR System also includes a quick-start setup so that you start a run immediately and enter plate information at a later time.

　　Sensitive LED-Based 3-Color Fluorescence Reading

　　The StepOne™ Real-Time PCR System utilizes a long-life LED-based optical system that can record fluorescence from FAM™/SYBR® Green, VIC®/JOE™, and ROX™ dyes. This cost-effective, 3-color, 48-well system delivers precise, quantitative Real-Time PCR results and saves data from all filters in every run without depending on a computer or plate setup. It can discriminate between 2 populations of 5,000 and 10,000 template copies of a TaqMan® assay with 99.7% confidence.

　　Compatible with Many Genomic Analysis Techniques

　　Perform a variety of standard and demanding genetic analysis research techniques on one instrument using the user friendly StepOne™ Real-Time PCR System and software. The StepOne™ System supports many Real-Time PCR applications, including the following:

　　• SNP Genotyping

　　• Gene Expression Analysis

　　• MicroRNA Expression

　　• Translocation Analysis

　　• Gene Detection

　　• Viral Load Analysis

　　The included StepOne™ Software supports a variety of analysis methods, including the following:

　　• Standard Curve (absolute quantitation)

　　• Relative Standard Curve

　　• Comparative Ct (ΔΔCt) (relative quantitation)

　　• Genotyping and Presence/Absence

　　• Melt Curve Analysis

　　• High Resolution Melting (additional software required)

　　Versatile Instrument Configuration Options

　　The ultra-compact footprint of a StepOne™ System can be installed in multiple distinct configurations, providing unmatched flexibility and convenience that can allow a fit in any laboratory (Fig 2). The StepOne™ Real-Time PCR System can be used via a PC, with a PC connected to a Local Area Network (LAN), or as a stand-alone instrument (PC-free). Each StepOne™ Real-Time PCR System is factory-calibrated for optical and thermal accuracy, so simply remarkable Real-Time PCR results can be obtained right out of the box.

　　Real-Time Data Monitoring, Dissemination, and Storage

　　The system measures amplification as it occurs, allowing you to monitor the progress of your experiment cycle by cycle, either on the machine or remotely. Your data is stored on the instrument itself, and can be viewed and stored automatically via remote access or transferred via email or a USB flash drive. Data can also be conveniently exported as PowerPoint®, Excel®, and graphical image files.