多功能酶标仪SpectraMax iD3 核心参数
吸光度线性：0-2 0.
精确度值：＜±0.010 OD
波长检测速度：光吸收96孔25秒
吸光值范围：230-1000 nm
制作曲线类型：参照软件
实验方法：终点法、动力法、扫描法
自动程度：全自动酶标仪
仪器种类：多通道酶标仪
检测器：PMT photodiode
波长范围：光吸收230-1000 nm；荧光250-850 nm；化学发光300-850 nm
仪器功能：多功能
产地类别：进口仪器
General specifications | |
| Dimensions (in.) | 15.79 (H) x 20.94 (W) x 23.54 (D) |
| Dimensions (cm) | 40.1 (H) x 53.2 (W) x 59.8 (D) |
| Weight | 88.1 lbs. (40 kg) |
| Power requirements | 100–240 VAC, 2 A, 50/60 Hz |
| Robotic compatible | Yes |
General performance | |
| Plate formats | 6 to 384 wells |
| Light source | Xenon flash lamp |
| Reading capabilities | Microplates, cuvettes (via adapter) |
| Detectors | Photo Multiplier Tube and Photodiode |
| Shaking | Orbital, Infinity |
| Temp. control | 5°C above ambient to 65°C |
| Temp. uniformity | ± 0.75°C |
| Temp. accuracy | ±1°C at 37°C set point |
| Spectral scanning | Abs, FI, Lum |
| Endpoint reading | Abs, FI, Lum |
| Kinetic reading | Abs, FI, Lum |
| Well scanning | Over 20 by 20 in all modes |
| Wavelength selection | 1.0 nm increments |
Standard read times (minutes:seconds) | ||
96 wells | 384 wells | |
| Absorbance | 0:25 | 1:25 |
| Fluorescence intensity | 0:17 | 0:53 |
| Luminescence | 0:26 | 1:01 |
Absorbance photometric performance | |
| Wavelength range | 230–1000 nm |
| Wavelength bandwidth | 4.0 nm |
| Wavelength accuracy | ± 2.0 nm |
| Wavelength repeatability | ± 1.0 nm |
| Photometric range | 0–4.0 OD |
| Photometric resolution | 0.001 OD |
| Photometric accuracy | < ±0.010 OD ±1.0%, 0–3 OD |
| Photometric precision | < ±0.003 OD ±1.0%, 0–3 OD |
| Stray light | < 0.05% @ 230 nm |
Fluorescence intensity performance | |
| Wavelength range | 250–850 nm |
| Wavelength selection | 1.0 nm increments |
| Dynamic range | > 6 decades |
Optimized Top sensitivity (fluorescein) | |
| 96 wells | 1 pM |
| 384 wells | 1 pM |
Optimized Bottom sensitivity (fluorescein) | |
| 96 wells | 2 pM |
| 384 wells | 2.5 pM |
Luminescence performance | |
| Wavelength range | 300–850 nm |
| Wavelength selection | Choice of simultaneous detection of all wavelengths or selection in 1.0 nm increments |
| Dynamic range | > 7 decades |
| Cross-talk | < 0.1% in white 96- and < 0.2% in 384-well microplates |
Optimized Sensitivity (ATP-Glow) | |
| 96 wells | 2 pM |
| 384 wells | 4 pM |
Injector system with SmartInject Technology (optional) | |
| Injectors | 2 |
| Read modes | Absorbance, fluorescence, luminescence |
| Dispense accuracy | ± 5% at 100 μL |
| Dispense precision | CV ≤ 2% at 100 μL |
| Dead volume | Injector tubing: 250 μL < 10 μL with Reverse Prime function |
| Detection limit | 20 amol ATP (“Flash“ luminescence using Promega ENLITEN® ATP Assay System) |
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