详细介绍
TissueLyser LT小型球磨仪
用于低到中通量样本破碎,适用于分子分析
同时破碎至多12个样本
仪器结构紧凑
配合可冷却的适配器,防止生物分子降解
可处理各种类型的样本,获得高重复性结果
与很多实验室流程兼容
TissueLyser LT是一款小型球磨仪,可同时快速、高效的破碎至多12个样品。该通量与自动化样品制备工作站QIAcube全自动核酸纯化仪*,可配合值得信赖的QIAGEN离心柱试剂盒使用。用不锈钢珠或玻璃珠高速震荡放置于2 ml微型离心管中的样品,实现同步破碎和匀浆。TissueLyser LT必须配合可冷却的适配器TissueLyser LT Adapter使用,该适配器在破碎过程中可固定试管。其他附件包括研磨珠和研磨珠分配器。
Effective tissue disruption.
Various rat tissues were disrupted using the TissueLyser LT or TissueLyser II. [A] DNA was purified from 25 mg samples on the QIAcube using the DNeasy Blood & Tissue Kit. DNA yields were determined using a spectrophotometer. [B] RNA was purified from 20 mg samples on the QIAcube using the RNeasy Fibrous Tissue Mini Kit (skin, heart, and lung) or RNeasy Lipid Tissue Mini Kit (brain). RNA yields were determined using a spectrophotometer.
高效的组织破碎。
使用TissueLyser LT或TissueLyser II破碎不同的大鼠组织。[A] 在QIAcube全自动核酸纯化仪上使用DNeasy Blood & Tissue Kit从25 mg样本中纯化DNA。利用分光光度计测定DNA产量。[B] 在QIAcube全自动核酸纯化仪上使用RNeasy Fibrous Tissue Mini Kit (皮肤、心脏和肺) 或RNeasy Lipid Tissue Mini Kit (大脑),从20 mg样本中纯化RNA。利用分光光度计测定RNA产量。
Pure RNA with high RIN values.
Rat heart and lung (20 mg each) were disrupted using the TissueLyser LT. RNA was purified on the QIAcube using the RNeasy Fibrous Tissue Mini Kit and then analyzed on the Agilent 2100 Bioanalyzer. The RIN values were 8.3 (heart) and 9 (lung).
纯化的RNA的RIN值高。
使用TissueLyser LT破碎大鼠的心脏和肺 (各20 mg)。在QIAcube全自动核酸纯化仪上使用RNeasy Fibrous Tissue Mini Kit纯化RNA,然后在Agilent 2100 Bioanalyzer上进行分析。RIN值分别为8.3 (心脏) 和9 (肺)。
Intact protein suitable for all types of analysis.
Various rat tissues (25 mg each) stabilized in Allprotect Tissue Reagent were disrupted using the TissueLyser LT or TissueLyser II. Total protein was purified using the Qproteome Mammalian Protein Prep Kit and then analyzed by western blotting. [A] Transfer membrane stained with Ponceau S, and [B] western blot. LT: TissueLyser LT; II: TissueLyser II; M: markers.
完整的蛋白质适合所有类型的分析。
使用组织溶解剂LT或组织溶解剂II破坏稳定在Allprotect组织试剂中的各种大鼠组织(每个25mg)。用Qproteome哺乳动物蛋白制备试剂盒纯化总蛋白,并用western印迹法进行分析。【A】用胭脂红染色的转移膜,和【B】western blot。LT:TissueLyser LT;II:TissueLyser II;M:标记。
High-quality DNA from animal tissues.
Various rat tissues (25 mg each) stabilized in Allprotect Tissue Reagent were disrupted using the TissueLyser LT or TissueLyser II. DNA was purified on the QIAcube using the DNeasy Blood & Tissue Kit, and then used in PCR with the HotStarTaq Plus Master Mix Kit and a PGK1 primer system. The 120 bp PCR product was analyzed on the QIAxcel using the QIAxcel DNA High Resolution Kit. LT: TissueLyser LT; II: TissueLyser II; M: markers.
来自动物组织的高质量DNA。
使用组织溶解剂LT或组织溶解剂II破坏稳定在Allprotect组织试剂中的各种大鼠组织(每个25mg)。用DNeasy血液和组织试剂盒在QIAcube上纯化DNA,然后用HotStarTaq+Master Mix试剂盒和PGK1引物系统进行PCR。用QIAxcel-DNA高分辨试剂盒在QIAxcel上分析120bp的PCR产物。LT:TissueLyser LT;II:TissueLyser II;M:标记。
High-quality DNA from plant tissues.
Various plant tissues (100 mg each) were disrupted in precooled adapters using the TissueLyser LT or TissueLyser II. DNA was purified on on the QIAcube using the DNeasy Plant Mini Kit and then analyzed on an agarose gel. LT: TissueLyser LT; II: TissueLyser II; M: markers.
来自植物组织的高质量DNA。
使用TissueLyser LT或TissueLyser II在预冷适配器中破坏各种植物组织(每种100 mg)。用DNeasy植物试剂盒在桥立方上纯化DNA,然后用琼脂糖凝胶进行分析。LT:TissueLyser LT;II:TissueLyser II;M:标记。
Intact RNA from plant tissues.
Various plant tissues (100 mg each) were disrupted in duplicate in precooled adapters using the TissueLyser LT. RNA was purified using the RNeasy Plant Mini Kit and then analyzed on an agarose gel. The sharp ribosomal RNA bands indicate purification of intact RNA.
从植物组织中纯化完整的RNA。
使用TissueLyser LT在预冷的适配器中破碎不同的植物组织 (各100 mg),重复两次。使用RNeasy Plant Mini Kit纯化RNA,然后进行琼脂糖凝胶电泳分析。清晰的核糖体RNA条带表示完整RNA的纯化。
Effective tissue disruption.
Various rat tissues were disrupted using the TissueLyser LT or TissueLyser II. [A] DNA was purified from 25 mg samples on the QIAcube using the DNeasy Blood & Tissue Kit. DNA yields were determined using a spectrophotometer. [B] RNA was purified from 20 mg samples on the QIAcube using the RNeasy Fibrous Tissue Mini Kit (skin, heart, and lung) or RNeasy Lipid Tissue Mini Kit (brain). RNA yields were determined using a spectrophotometer.
TissueLyser LT.
Performance
TissueLyser LT可高效破碎人类、动物和植物组织、细菌和酵母,确保后续DNA、RNA和蛋白质纯化中产量重复性好(参见“组织破坏”)。心脏和大脑等难裂解组织,也可使用TissueLyser LT处理。破碎之后DNA、RNA和蛋白质保持完整(参见“纯化的RNA的RIN值高“、“完整的蛋白质适合所有类型的分析“、“来自动物组织的高质量DNA“、“来自植物组织的高质量DNA“和“从植物组织中纯化完整的RNA“),确保下游应用中可靠的分析。
TissueLyser LT的破碎效果与TissueLyser II相当(参见“高效的组织破碎“、“完整的蛋白质适合所有类型的分析“、“来自动物组织的高质量DNA“和“来自植物组织的高质量DNA“)。
Principle
基因分型、基因表达分析和蛋白质组学研究均要求对生物样本进行高效破碎,以确保获得高产的DNA、RNA和蛋白质。TissueLyser LT可快速、彻底的破碎样本,彻底释放生物分子,并可同时对样本进行匀质化,协助后续使用的QIAGEN试剂盒进行纯化(参见下表)。
QIAGEN提供从样本收集到DNA、RNA和蛋白质纯化的完整解决方案,TissueLyser LT是其中关键的组成部分。TissueLyser LT适用于QIAGEN手动样本制备试剂盒(参见下表),也是QIAGEN中通量样本制备和分析自动化解决方案的补充(参见下表)。包括应用QIAGEN离心柱试剂盒自动纯化样品的QIAcube全自动核酸纯化仪,以及对DNA和RNA进行毛细管凝胶电泳的QIAxcel。