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布鲁克 timsTOF™ Pro 捕集离子淌度质谱

tel: 400-6699-117 2005

布鲁克液质联用仪, 这致使近乎100%的占空比,使这种平行堆积和连续碎裂(PASEF)技术在酶促消化的蛋白质混合物的可重复纳流LC-MS分析......

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QTOF分辨率:50,000

近乎100%的占空比

参考招标参数:项目名称: 北京大学医学部捕集离子淌度飞行时间蛋白分析系统招标采购项目 招标编号: OITC-G190310421

技术规格及要求

1. 捕集离子淌度飞行时间蛋白分析系统:

★1.1 具备高分辨捕集离子淌度分离功能,离子淌度分辨率≥60并且能提供碰撞截面积(CCS)数值。

★1.2 质谱采样频率:MS≥40 Hz,MS/MS采集速度≥100 Hz。

#1.3 质谱分辨率:在高采集速度(≥40 Hz)下,分辨率≥50,000(@m/z 1222,FWHM)

1.4准确度:采用内标校准,平均误差 ≤ 0.8 ppm;采用外标校准:平均误差 ≤ 2 ppm。

1.5 适用于定性定量分析的高分辨提取离子色谱技术:在常规色谱峰中,高分辨提取离子色谱(hrEIC)技术保证小于1.0 mDa误差的稳定。

1.6 质量范围: 20-40,000 m/z。

#1.7 淌度模式灵敏度: MS:1pg/uL利血平,信噪比≥100: 1 RMS;MS/MS:2.5 fmol多肽Glu-Fibrinopeptide B时,二价离子碎片的y离子中最强峰信号大于800,信噪比大于100:1。

#1.8 同位素峰型保真:能提供真实同位素峰型分布信息,同位素峰误差≤2%。

#1.9 数据采集模式:数据采集软件可以通过碰撞截面积(CCS)选择离子并进行选择性累积。

#1.10 飞行管温度补偿模式:具有水冷控温装置,主动温度补偿功能以保证质量轴稳定。

2. 纳升液相色谱:

2.1 与捕集离子淌度飞行时间蛋白分析系统兼容性好,可高效地进行液质联用分析。

2.2 进样体积范围1-18 μL,并且进样体积RSD < 0.5% (10 μL)

2.3 梯度模式下,色谱流速范围100–2000 nL/min

2.4 色谱仪耐受的最高压力 ≥ 900 bar

2.5 纳升色谱性能稳定,进行蛋白质组研究时要求RT RSD < 0.5%,FWHM < 8.0s,Area RSD < 15.0%

2.6 配备柱温箱,温度范围30-60°C

3、售后服务:

3.1 设备安装、调试和验收:卖方应在合同生效后的1个月内向用户提供详细的安装要求并提供技术咨询;仪器到达用户所在地,在接到用户通知后一周内进行安装调试,直至通过验收。投标方承担有关费用。

3.2 技术培训:在用户所在地对用户进行3人次为期1周的培训。培训内容包括仪器的技术原理、操作、数据处理、基本维护等。

★3.3 保修期:卖方提供3年的免费保修,保修期自仪器验收签字之日起计算。保修期间维修及零件更换费用由卖家负担。

3.4 维修响应时间:保修期内卖方应在24小时内到达维修现场;保修期结束后到达现场时间同前。维修服务包括电话指导和现场维修。 

3.5 要求厂家在中国设有1个以上固定维修站,并配有专业维修工程师,保证提供及时优质的售后服务。

4. 订购数量:1套

5. 交货地点:用户指定地点

6. 交货日期: L/C后90天内

★7所投产品需为现有成型产品,不得为特供机型,提供产品彩页。

★8.“#”项指标代表重要指标,投标产部不满足将被减分,关键指标(“★”指标)是必须满足的指标,如不满足将予以废标。


Novel dual TIMS design delivers near 100% duty cycle

The novel design allows incoming ions to be accumulated in parallel in the first section and for ions to be released dependent on their mobility from the second section of the dual TIMS analyzer in a process called parallel accumulation serial fragmentation or PASEF. This allows for near 100% duty cycle.

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PASEF digs deeper into the proteome

PASEF delivers unmatched effective MS/MS acquisition rates and sensitivity by interfacing the time focusing power of the TIMS dimension with ultra-high resolution QTOF technology. Due to the sensitivity gains from PASEF, it is possible to analyze samples with low cell populations and shorter gradients can be used while identifying more peptides and their post-translational modifications (PTMs).

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Power of TIMS separation: (A) Shotgun proteomics data feature two dimensions at the MS level, the m/z and the retention time (MaxQuant viewer). Illustration of the extremely high complexity of shotgun proteomics data from a HeLa digest over a 90 min gradient. (B) A large number of co-eluting peptides that only differ slightly in mass within a 2 min retention time slice. (C) Within a 2 Th isolation window peptides are not distinguishable based on their m/z. (D) But peptides can be separated based on their trapped ion mobility.

Enabling record breaking speed with PASEF

The timsTOF Pro was engineered by Bruker experts for scientists to enable breakthrough discoveries in shotgun proteomics. Using parallel accumulation, all ions that enter the timsTOF Pro can be analyzed. In addition, higher ion transmission is provided by the newly designed TIMS synchronized quadrupole. The high speed collision cell and ultra-high resolution TOF enables high MS and MS/MS spectra quality at high speeds.

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timsTOF Pro for high sensitivity shotgun proteomics

Recent advances in proteomic technologies have made MS-based proteomics a central research tool. However, truly complete proteomes are still elusive, mainly due to the high complexity and dynamic range of biological samples. Analyses using low sample amounts or where a range of post-transla-tional modifications (PTMs) need to be located additionally remain challenging. With the timsTOF Pro, deep proteome coverage can be achieved without the need for large sample amounts ( > 1 µg), reducing the sample preparation costs and making it very attractive for clinical proteomics when sample amounts are limited. Moreover, cleaner MS/MS spectra can be generated by the additional dimension of separation.

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Higher throughput and more PTMs revealed using PASEF

Due to the extremely high data acquisition speed provided by PASEF on the timsTOF Pro without a loss in sensitivity and resolution, typical proteomic analyses can be performed even faster, increasing the throughput of biological experiments tremendously. In addition, PTMs can be better separated from each other using TIMS resulting in a higher identification rate.

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地址:北京海淀区西小口路66号中关村东升科技园B-6号楼C座8层

电话:400-6699-117 转 2005

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