ProZyme Gly-Q糖基分析系统

ProZyme公司推出Glycan-Q系统用于综合的N-糖分析

加利福尼亚州海沃德,2016年11月14日/PRNewswire-iReach发布，ProZyme公司, 糖生物学领先的试剂和工具制造商, 今天宣布推出Glycan-Q糖分析系统, 一个集成的高通量平台, 可进行用户友好的糖基分析。Glycan-Q系统利用ProZyme在快速糖样品制备和分析级糖基标准品方面的专长, 并结合小型毛细管凝胶电泳 (CGE) 仪器，后者装载有为简化糖数据分析而设计的定制化软件。

“我们很高兴推出ProZyme 的第一款仪器产品, “ ProZyme 的总裁兼首席执行官Sergey Vlasenko博士说。“我们在过去几年中加快了糖样品制备的工作, 但数据分析仍然是一个瓶颈。通过我们的新系统, 生物制药公司的细胞线开发和分析组可以更快地确定样品的糖谱, 同时降低总体成本。我相信该系统可以帮助研究人员运行 glycomic（糖组学）分析的大型数据集, 包括临床样本。

蛋白质糖基化是一种翻译后修饰, 可影响治疗蛋白的有效性和安全性, 如对单克隆抗体, N-糖基化是一个关键的质量属性。蛋白质糖基化也可以作为特定疾病状态的指示。传统的N-糖分析方法包括缓慢的、耗费大量人力的步骤来进行样品制备、糖分离和数据分析。ProZyme 推出了Glycan-Q系统，实现了糖分析的高通量和简单化，可用于生物治疗药物的开发, 并帮助发现糖生物标志物。

在Glycan-Q工作流的前端, ProZyme Gly-X化学酶和PNGase（聚糖酶）的作用下，可5分钟内从糖蛋白中释放N-糖，释放出的N-糖立即用荧光染料标记, 然后用CGE分离。整个样品准备过程可在45分钟内完成, 并且是自动化的。荧光标记的N-糖然后在Glycan-Q CGE 仪器上分离, 每个样本运行时间为2分钟, 允许在一个8小时的工作日内准备和分析96个样本。该仪器具有易于更换的Glycan-Q卡盒, 含有毛细管和凝胶基质。

Glycan-Q仪器控制和N-糖数据分析由定制构建的Glycan-Q管理器软件完成。电泳标准品是与每个样本共同注入的, 这与阶梯标准品一起实现了对每个样本峰值的自动数据对准和葡萄糖单位 (GU) 值的计算。然后将样本的GU值与内置和可自定义的糖参考库进行比较, 以进行峰值分配。通过 ProZyme 的糖标准品和外切糖苷（exoglycosidase）来增强分析效果。

Gly-Q系统用于N-糖样品制备、分离和分析，由ProZyme 销售。要了解更多关于Gly-Q系统的信息, 请访问 prozyme.com:

Gly-Q系统糖分析系统

Gly-Q管理者软件

Gly-X N-糖快速释放，InstantQ 套件标记

Complete Integrated System for N-Glycan Analysis 
The Gly-Q system is designed as a complete system for rapid analysis of oligosaccharides such as N-glycans, and includes an instrument, replaceable cartridges, software and sample preparation kits. The instrument is comprised of a capillary gel electrophoresis (CGE) core integrated with a high sensitivity LED-induced fluorescence (LEDIF) detector and 96-well autosampler. The replaceable Gly-Q cartridge automatically regenerates the capillary gel between runs and can be used for up to 150 sample injections. Gly-Q Manager software provides integrated system control, data analysis and data export functions. InstantQ™ dye, provided in the Gly-X N-Glycan Rapid Release and Labeling with InstantQ kit, is specifically designed for N-glycan separation and detection using the Gly-Q instrument. 

Gly-X Technology with InstantQ Dye 
ProZyme Gly-X 5-minute deglycosylation with N-Glycanase™ (PNGase F) is enabled by a proprietary denaturant reagent that enhances exposure of N-glycan sites to rapid enzymatic cleavage. Gly-X achieves complete, unbiased in-solution deglycosylation of up to 40 μg glycoprotein per sample. Followed immediately by labeling with InstantQ dye (1 minute), and a simple clean-up step (elution into water), the glycan samples are ready for analysis using the Gly-Q instrument. With deglycosylation and labeling carried out in solution, the method is simple, rapid (96 samples in 1 hour) and suitable for automation. The Gly-X with InstantQ sample preparation kit is available in a modular format with reagents to prepare from 24 to 96 samples. InstantQ labeled standards and controls are also available

CE Designed for Rapid Glycan Analysis
Gly-Q is a small, simple, user friendly capillary electrophoresis (CE) system with an easily-replaceable gel cartridge. The 147 mm capillary mounted into the cartridge contains a gel matrix that is replenished from the top reservoir of the cartridge automatically between sample runs. The reservoir provides sufficient gel for up to 150 sample separations. Gly-Q Manager Software provides a simple sample set-up in both a 96-well plate and table view. Standards, controls and system buffers are loaded into dedicated wells on the instrument. After sequence set-up, place the InstantQ labeled samples on the autosampler and press start. Special solvents and diluents are not required.

Automated & Simplified for Rapid Screening

Gly-Q Manager software provides simplified data analysis that enables rapid screening of samples as well as more detailed analysis. The software automatically aligns electropherograms with GU values and, where possible, assigns N-glycans. The ProZyme global processing method contains GU values for 46 N-glycans. This component table can be easily modified to contain any set of expected N-glycans and saved as a new processing method. Entire data sets or individual samples can be re-processed without loss of the original analysis. The interactive interface allows one to zoom in on areas of interest, and to compare data across samples and experiments. Peak name, GU units, relative peak area, total area and other calculations are displayed for each sample in the table. Data can be exported as QDF, PDF, CSV as well as CDF for making reports in additional software packages.