浙江省微生物生化与代谢工程重点实验室
400-6699-117转1000
主要获奖成果
[1] 李永泉、张建国、谢志鹏、吕龙贤、喻晓蔚、管文军等，酶法合成与分离耦合技术在有机酸产品开发中的应用，教育部科技进步奖一等奖，2009年12月
[2] 张建国、李永泉、谢志鹏、吕龙贤、管文军、喻晓蔚等，生物反应与分离耦合技术在轻工医药中的应用，浙江省科学技术奖一等奖，2008年12月
[3] 李永泉、杨豪杰、吕龙贤、许海军、管文军、马国平、毛旭明、倪利强，羊毛织物生物后整理技术的应用开发，中国商业联合会科学技术进步奖二等奖，2009年12月。
[4] 张建国等，酶法合成与分离耦合技术在有机酸产品开发中的应用，2010年度杭州市科技创新十佳产学研合作项目；
[5] 刘珊林、施冬云、龚兴国、沈新南、刘冠中，活性氧对肿瘤细胞生长及相关基因和信号通路调控作用的研究，高等学校自然科学奖二等奖，2009年12月
[6] 徐志南、龚兴国、方向明、林建平、岑沛霖、卢敏、蔡谨、傅维琦、黄磊、陈海琴，体内和体外高效合成功能性异源蛋白的理论基础和关键技术，浙江省科学技术奖二等奖，2009年12月
[7] 李永泉，杨豪杰，管文军，倪利强，吕龙贤，李擎文，周宏，马国平，许海军，羊毛织物复合酶制剂及其在羊毛织物绿色后整理中的应用：浙江省科学技术奖二等奖，2007年12月25日
[8] 张耀洲、吴祥甫、金勇丰等，家蚕“生物反应器”生产生物制品的方法，国家技术发明二等奖，2005年1月。
[9] 李永泉、沙兴宝、姚庆才、龚兴国等，纺织生物活性柔软剂在羊毛织物后整理中的应用，浙江省科技进步奖二等奖，2001年12月。
近三年授权发明专利
[1] 李永泉、吕龙贤、周宏、闵杰，一种产角蛋白酶的粘金黄杆菌及其分离方法，中国发明专利，授权公告日2011.3.9，专利号：ZL200810060637.4
[2] 李永泉、杜艺岭、陈时飞、樊伟明、程良英，一种恰塔努加链霉菌及培养方法，中国发明专利，授权公告日2011.5.18，专利号：ZL200810162820.5
[3] 龚兴国、郦剑勇，从禽畜血中提取血红蛋白和超氧化物岐化酶的方法, 中国发明专利，授权公告日2011.4.13，专利号：ZL200810120961.0
[4] 龚兴国、郦剑勇，血红素和脱色血球蛋白粉的生产方法，中国发明专利，授权公告日2011.7.22，专利号：ZL200810120120963.X
[5] 李永泉、吕龙贤、管文军、沈珉豪，一种液体深层发酵生产角蛋白酶的方法，中国发明专利，授权公告日2010.2.3，专利号：ZL200810060639.3
[6] 龚兴国、郦剑勇，一种纯化血红素的方法，中国发明专利，授权公告日2010.9.29, 专利号：ZL200810120120962.5
[7] 朱成钢、史锋，含核酸适配子的检测试剂及其制备方法和用途，中国发明专利，授权公告日2010.5.26，专利号：ZL200710067163.1
[8] 管文军、葛浣溪、李红、陈裕泉，基于碳纳米管的针式生物传感器及其制作方法，中国发明专利，授权公告日2009.7.29，专利号：ZL200610053956.3
[9] 詹金彪、夏 铮、王 峰、徐小蓉、黄建松，芋螺毒素M VII A突变体及制备和应用，中国发明专利，授权公告日2009.8.26，专利号：ZL200610052400.2.
[10] 詹金彪、邹 媛、黄建松、冯微宏，一种整合酶抑制肽及其在艾滋病药物制备中的应用，中国发明专利，授权公告日2009.9.23，专利号：ZL200710067526.1
[11] 李永泉、孙一晟、许勤勤、吴斌，大团囊虫草菌中的倍半萜类化合物及其用途，中国发明专利，公开公告日2011.11.2，申请号：201110111071.5
[12] 朱健、陈晓霞、谢祥茂、陈俊勇，一种链霉菌及其应用，中国发明专利，公开公告日2010.8.4，申请号：200910028593.1
[13] 李永泉、江辉，2012，基因工程菌恰塔努加链霉菌L11的构建及培养方法，中国发明专利，申请号201210063650.6
[14] 张耀洲，李霁，金勇丰，一种治疗阿尔茨海默病的融合蛋白及其制备方法，中国发明专利，公开公告日2012.4.25，申请号CN 200810135937
近三年主要SCI论文
[1] Xu-Ming Mao, Ning Sun, Feng Wang, Shuai Luo, Zhan Zhou, Wei-Hong Feng, Fang-Liang Huang, and Yong-Quan Li*. Dual Positive Feedback Regulation of Protein Degradation of an Extra-cytoplasmic Function σ Factor for Cell Differentiation in Streptomyces coelicolor. The Journal of Biological Chemistry (2013), 288(43), 31217–31228. (5-Year IF 5.023)
[2] Hui Jiang, Yue-Yue Wang, Xin-Xin Ran, Wei-Ming Fan, Xin-Hang Jiang, Wen-Jun Guan*, and Yong-Quan Li*. Improvement of Natamycin Production by Engineering of Phosphopantetheinyl Transferases in Streptomyces chattanoogensis L10. Applied and Environmental Microbiology (2013), 79(11), 3346–3354. (5-Year IF 4.406)
[3] Xu-Ming Mao, Zhi-Hao Sun, Bi-Rong Liang, Zhi-Bin Wang, Wei-Hong Feng, Fang-Liang Huang and Yong-Quan Li*. Positive Feedback Regulation of stgR Expression for Secondary Metabolism in Streptomyces coelicolor. Journal of Bacteriology (2013), 195(9). (5-Year IF 3.586)
[4] Zhang L, Cui J, Leonard M, Nephew K, Li Y*, Zhang X*. Silencing MED1 Sensitizes Breast Cancer Cells to Pure Anti-Estrogen Fulvestrant In Vitro and In Vivo. PLoS ONE, (2013); 8(7):e70641. (5-Year IF 4.244)
[5] Liang He , Pengfei Ji , Junwen Cheng , Yanbin Wang , Hua Qian , Weiqi Li , Xingguo Gong*, Ziyu Wang. Structural characterization and immunostimulatory activity of a novel protein-bound polysaccharide produced by Hirsutella sinensis. Food Chemistry (2013), 141: 946–953. (5-Year IF 4.072)
[6] Yang H, He X, Yang J, Deng X, Liao Y, Zhang Z, Zhu C, Shi Y, Zhou N*. Activation of cAMP-response element-binding protein is positively regulated by PKA and calcium-sensitive calcineurin and negatively by PKC in insect. Insect Biochem Mol Biol. (2013). 43(11):1028-1036. (5-Year IF 3.62)
[7] Yang J, Huang H, Yang H, He X, Shi Y，Alatangaole Damirin, Shi L, and Zhou N*. Specific Activation of the G Protein-Coupled Receptor BNGR-A21 by the Neuropeptide Corazonin from the Silkworm, Bombyx mori, Dually Couples to the Gq and Gs Signaling Cascades. The Journal of Biological Chemistry (2013), 288: 11662-11675. (5-Year IF 5.023)
[8] Zheng C, Chen L, Chen X, He X, Yang J, Ying Shi, Naiming Zhou*. The Second Intracellular Loop of the Human Cannabinoid CB2 Receptor Governs G Protein Coupling in Coordination with the Carboxyl Terminal Domain. PLoS ONE (2013), 8: e63262. (5-Year IF 4.244)
[9] Wang X, Li G, Yang Y, Wang W, Zhang W, Pan H, Zhang P, Yue Y, Lin H, Liu B, Bi J, Shi F, Mao J, Meng Y, Zhan L, Jin Y. An RNA architectural locus control region involved in Dscam mutually exclusive splicing. Nat Commun. 2012;3:1255. doi: 10.1038/ncomms2269.
[10] Yue Y. Li G. Yang Y. Zhang W. Pan H. Chen R. Shi F. Jin Y. Regulation of Dscam exon 17 alternative splicing by steric hindrance in combination with RNA secondary structures. RNA Biology. (2013), 10:12, 1–12. (5-Year IF 5.436)
[11] Jiang X, Zhu Z, Sun Z, Wang L, Zhou L, Miao H, Zhang Z, Shi F, Zhu C*. The development of an indirect competitive immunomagnetic-proximity ligation assay for small-molecule detection. Analyst, (2013), 138, 438. (5-Year IF 3.904)
[12] Zhan Zhou, Yong-Quan* Li, Yufeng Wang*, Genome plasticity and systems evolution in Streptomyces, BMC Bioinformatics. (2012), 13 (Suppl 10): S8 (5-Year IF 3.51)
[13] Xiaoxian Guo, Jingkai Li, Tanjun Wang, Zhenhua Liu, Xin Chen, Yudong Li, Zhenglong Gu, Xuming Mao, Wenjun Guan*, Yongquan Li*. A Mutation in Intracellular Loop 4 Affects the Drug-Efflux Activity of the Yeast Multidrug Resistance ABC Transporter Pdr5p. PLoS ONE, (2012), 7(1):e29520 (5-Year IF 4.24)
[14] W.Wang, X.Zhou, W.L.He, Y.Fan, Y.Z.Chen, X.Chen*. The Inter-Protein Scoring Noises in Glide Docking Scores. Proteins (2012), 80:169-183 (5-year IF 3.103).
[15] Yiling Du, Shanzhen Li, Zhan Zhou, Shifei Chen, Weimin Fan, Yongquan Li*. The pleitropic regulator AdpAch is required for natamycin biosynthesis and morphological differentiation in Streptomyces chattanoogensis. Microbiology-SGM (2011), 157: 1300 – 1311.(5-Year IF 3.289)
[16] Yiling Du, Xueling Shen, Pin Yu, Linquan Bai, and Yongquan Li*. Gamma-Butyrolactone Regulatory System of Streptomyces chattanoogensis Links Nutrient Utilization Metabolism and Development. Applied and Enviromental Microbiology (2011), 77(23):8415–8426 (5-Year IF 4.529)
[17] Wei Chen*, Zhao Zhao, Yongquan Li**. Simultaneous increase of mycelial biomass and intracellular polysaccharide from Fomes fomentarius and its biological function of gastric cancer intervention. Carbohydrate Polymers (2011)，85：369–375(5-Year IF 3.659)
[18] Weihong Feng, Xuming Mao, Zhenhua Liu, Yongquan Li*, The ECF sigma factor SigT regulates actinorhodin production in response to nitrogen stress in Streptomyces coelicolor，Applied Microbiology and Biotechnology (2011), 92:1009–1021. (5-Year IF 3.583)
[19] Xiaoxian Guo, Jingkai Li, Zhenhua Liu, Xin Chen, Yudong Li，Zhenglong Gu, Xuming Mao, Wenjun Guan*, Yongquan Li*. A Mutation in intracellular loop 4 affects the drug-efflux activity of the yeast multidrug resistance ABC transporter Pdr5p. PLoS ONE (2012), 7(1): e29520. (5-Year IF 4.61)
[20] Ying Shi, Haishan Huang, Xiaoyan Deng, Xiaobai He, Jingwen Yang, Huipeng Yang, Liangen Shi, Lijuan Mei, Jimin Gao and Naiming Zhou*. Identification and Functional Characterization of Two Orphan G-Protein Coupled Receptors for Adipokinetic Hormones from Silkworm Bombyx mori. Journal of Biological Chemistry (2011), 286(49): 42390–42402. (IF 5.498)
[21] Guo Li, Xiaoyan Deng, Qi Zhou, Jiansong Luo, and Jeffrey Benovic, Naiming Zhou.* Distinct Kinetic and Spatial Patterns of Gi-Initiated PKC- and EGFR- Dependent Activation of Extracellular Signal-Regulated Kinases 1 and 2 by Human Nicotinic Acid Receptor GPR109A. Journal of Biological Chemistry (2011), 286(36): 31199-31212. (IF 5.498)
[22] Yaping Zhang, Wen Yang, Linjie Chen, Guo Li, Haishan Huang, Naiming Zhou*. Development of a Novel Assay Based on DnaE intein for Quantitative Analysis of GPCR Internalization. Anal Biochem. (2011), 417(1):65-72. (IF 3.236)
[23] Haishan Huang, Xiaoyan Deng, Xiaobai He, Wen Yang, Guo Li, Ying Shi, Liangen Shi, Jimin Gao, and Naiming Zhou*. Identification of Distinct C-terminal Domains of the Bombyx Adipokinetic Hormone Receptor that are Essential for Receptor Export, Phosphorylation and Internalization. Cellular Signalling (2011), 23(9):1455-1465. (5-year IF 4.114)
[24] Yang Y, Zhan L, Zhang W, Sun F, Wang W, Tian N, Bi J, Wang H, Shi D, Jiang Y, Zhang Y, Jin Y*. RNA secondary structure in mutually exclusive splicing. Nat Struct Mol Biol (2011), 18: 159-68. (IF 13.685)
[25] Tian N, Yang Y, Sachsenmaier N, Muggenhumer D, Bi J, Waldsich C, Jantsch MF, Jin Y*. A structural determinant required for RNA editing. Nucl Acids Res (2011) , 39(13):5669-81. (IF 7.836)
[26] Jin Y*, Yang Y, Zhang P. New insight into RNA secondary structure on alternative splicing of pre-mRNA. RNA Biology (2011) , 8(3):450-7. (IF 5.597)
[27] Min-Kan Lu, Xing-Guo Gong* and Kun-Liang Guan(2011)，mTOR in podocyte function: is rapamycin good for diabetic nephropathy? Cell Cycle(2011), 10(20). (5-year IF 4.499)
[28] Yong Qin, Xiang Pan, Tiantian Tang, Liang Zhou, Xingguo Gong*，Anti-proliferative effects of the novel squamosamide derivative (FLZ) on HepG2 human hepatoma cells by regulating the cell cycle-related proteins are associated with decreased Ca2+/ROS levels. Chemico-Biological Interactions (2011), 10. (IF 3.31)
[29] Hongyun Tang, Yong Qin, Jianyong Li and Xingguo Gong*. The scavenging of superoxide radicals promotes apoptosis induced by a novel cell-permeable fusion protein, sTRAIL:FeSOD, in tumor necrosis factor-related apoptosis-inducing ligand-resistant leukemia cells，BMC Biology (2011), 9:18doi:10.1186/1741-7007-9-18. (5-year IF 5.479)
[30] Haifeng Pan, Zhipeng Xie, Wenna Bao, Yongqing Cheng, Jianguo Zhang*, Yongquan Li. Site-directed mutagenesis of epoxide hydrolase to probe catalytic amino acid residues and reaction mechanism. FEBS Letters (2011), 585: 2545-2550. (IF 3.601)
[31] Fang M, Wang J, Han S, Hu ZY, Zhan JB, Ling SC, Rudd JA, Geng Y. Protective effects of w-conotoxin on Amyloid-β-induced damage in PC12 cells. Toxicol Lett (2011) , 206(3):245-346. (IF 3.58)
近三年承担的主要项目
[1] 李永泉，微生物药物的高效合成生物技术研究与应用，课题编号：2012AA02A706，863计划重大项目课题，201211-201512
[2] 李永泉，微生物药物创新与优产的人工合成体系，批准号：2012CB721005，973计划子课题-药物合成途径解析与优化，201201-201612
[3] 李永泉，纳他霉素次级代谢调控的分子机制研究，批准号：LZ12C01001，浙江省自然科学基金重点项目，201201-201512
[4] 李永泉，抗多重耐药细菌达托霉素的新药开发，批准号：2011ZX09202-101-11，国家新药创制重大专项，201101-201312
[5] 李永泉，新型食品防腐剂纳他霉素的产业化研究开发，批准号：2011BAD23B05-2，国家科技支撑计划子课题，201101-201512
[6] 李永泉，
天蓝色链霉菌sigma因子SigT的调控机制研究，批准号：31070040， 国家自然科学基金面上项目，201101-201312
[7] 李永泉，基因工程药物研发平台建设，项目编号H20103387，合同号10-508203-007，重大横向(浙江杰特投资有限公司)，201012-201212
[8] 周耐明，针对重要疾病的靶标发现和成药性功能确证，863计划子项目，201201- 201512
[9] 周耐明，重要G蛋白偶联受体的结构与功能研究及配体发现- GPCR在重大疾病发生发展中的作用和机理，863计划子项目，201201-201412
[10]周耐明，烟酸受体GPR109A介导的信号转导通路对脂解作用和皮肤潮红的调节及分子机制，国家自然科学基金面上项目，201201-201512
[11] 周耐明，GPCR在重大疾病发生发展中的作用和机理，批准号：2012CB910402，科技部，201401-201712
[12] 龚兴国，以串联表达方式制备天然人胸腺素α1（上海育臣生物工程技术有限公司），合同号：11-508203-001，重大横向， 201105-201505
[13] 金勇丰，RNA编辑影响RNA二级结构介导可变剪接的调控机制研究，批准号：31270844，国家自然科学基金面上项目，201401-201712
[14] 金勇丰，新型I型糖尿病口服药物的研制和中试，批准号：2012ZX09102301-009，科技部，201401-201712
[15] 易文，新型酶-化学联合方法标记肿瘤特异性糖链，批准号：31270865，国家自然科学基金面上项目，201301-201612
[16] 易文，肿瘤的糖生物学批准号：31322019，国家自然科学基金优秀青年项目，201401-201712
[17] 管文军，酿酒酵母ABC转运蛋白Pdr5p影响细胞麦角甾醇平衡的分子机制研究，批准号：31170032，国家自然科学基金面上项目，201201-201412
[18] 管文军，血糖检测与胰岛素注射微系统，863计划子项目，20101-201212
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