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基因表达差异显示实验>Analysis of Nucleic Acids by Tandem Hybridization on Oligonucleotide Microarrays
Analysis of Nucleic Acids by Tandem Hybridization on Oligonucleotide Microarrays
关键词: analysis nucleic来源: 互联网
Oligonucleotide arrays (also known as DNA chips, gene chips, or genosensor chips) are emerging as a powerful research tool in nucleic acid sequence analysis. Several technical challenges remain to be solved, however, before oligonucleotide arrays can reach their full potential and be implemented in a robust fashion. Some of these challenges are as follows:
1. | The need to generate single-stranded target nucleic acids in order to achieve optimal hybridization signals. |
2. | Spontaneous formation of secondary structure in the single-stranded target nucleic acid, causing certain stretches of target sequence to be poorly accessible to hybridization. |
3. | Imperfect specificity of hybridization, making it difficult or impossible to distinguish between certain sequence variations. |
4. | The strong influence of base composition on the stability of short duplex structures, making it difficult to use an extensive array of oligonucleotides (differing in base composition) to analyze a nucleic acid sample under a single hybridization condition. |
5. | Multiple occurrence of sequences complementary to short oligonucleotide probes within the nucleic acid sample, limiting the genetic complexity of a nucleic acid sample that can be analyzed by arrays of short oligonucleotide probes. |
6. | The need to label each nucleic acid analyte prior to hybridization to the DNA probe array, a significant factor in the overall time and cost of analysis. |
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