Microaspiration of Esophageal Gland Cells and cDNA Library Construction for
Microaspiration of Esophageal Gland Cells and cDNA Library Construction for Identifying Parasitism Genes of Plant-Parasitic Nematodes
Identifying parasitism genes encoding proteins secreted from a
plant-parasitic nematode’s esophageal gland cells and injected through
its stylet into plant tissue is the key to understanding the molecular
basis of nematode parasitism of plants. Parasitism genes have been
cloned by directly microaspirating the cytoplasm from the esophageal
gland cells of different parasitic stages of cyst or root-knot nematodes
to provide mRNA to create a gland cell-specific cDNA library by long-distance reverse-transcriptase polymerase chain reaction. cDNA
clones are sequenced and deduced protein sequences with a signal
peptide for secretion are identified for high-throughput in situ
hybridization to confirm gland-specific expression.