DNA微序列技术
· Protocols for Making Drosophila Arrays (Stanford U.)
Detailed protocol for making arrays including PCR Amplification of cDNAs for Printing, Polylysine Slide Preparation, "Post-Processing" and Direct Labeling of cDNA probes.
· Preparation of Slides (Brown Lab)
· Preparation of DNA Samples (Brown Lab)
· Post-Processing of Arrays (Brown Lab)
· Preparation of Fluorescent DNA Probe from Yeast mRNA (Brown Lab)
· Preparation of Fluorescent Probe from Human RNA (Brown Lab)
· Preparation of Fluorescent Probe from E. coli RNA (Brown Lab)
· Preparation of Fluorescent DNA Probe from Genomic DNA (Brown Lab)
· Cyanine Dye HPLC Purification (Brown Lab)
· Modified Eberwine ("antisense") RNA Amplification Protocol (Brown Lab)
· Hybridization of Arrays (Brown Lab)
· Preparation of Total RNA from Cultured Human Cells (Telechem)
· Preparation of PolyA+ mRNA from Total Human RNA (Telechem)
· Amplification and Purification of cDNAs for Microarray Manufacture (Telechem)
· Microarray Manufacture and Processing (Telechem)
· Generating Control mRNAs by In Vitro Transcription (Telechem)
· Generating Fluorescent cDNA Controls by Linear PCR (Telechem)
· Preparation of Fluorescent Probes from Total Human mRNA (Telechem)
· cDNA Microarray Hybridization and Washing (Telechem)
· Gene Expression Analysis with Microarrays (Telechem)
· Mutation Detection with Oligonucleotide Microarrays (Telechem)
· Comparative Gene Expression Study Using Microarray (Jeremy Buhler)
· Microarray Hybridization Protocols (Childs / Kucherlapati Lab)