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2019.4.22

Working concentrations and stock solutions.

AntibioticsStock solutionWorking concentration:Strict controlWeak controlmg/ml: µg/mldilutionµg/mldilutionAmpicillin50 (H2O*)-20oC200.4 µl/ml60-1251.2-2.5 µl/mlCarbenicillin50(H2O, 50%EtOH)-20oC200.4 µl/ml60-2001.2-4 µl/mlChloramphenicol34(EtOH, MetOH)-20oC250.7 µl/ml1705 µl/mlD-cycloserine*10(0.1M NaP pH8.0)+4oC20020 µl/ml Gentamycin10 (H2O)-20oC151.5 µl/ml Hygromycin B10 (H2O)-20oC101 µl/ml40040 µl/mlKanamicin50 (H2O)+4oC--20oC100.2 µl/ml501 µl/mlKasugamycin10 (H2O)-20oC1000100 µl/ml Nalidixic acid5(H2O, pH to 11 /NaOH)-20oC153.0 µl/ml Neomycin10 (H2O)-20oC10010 µl/ml80080 µl/mlRifampicin**34 (MetOH)-20oC1504.4 µl/ml Spectinomycin10 (H2O)-20oC10010 µl/ml Streptomicin50 (H2O)-20oC100.2 µl/ml501 µl/mlTetracycline***5 - 12.5(EtOH, 70%EtOH)-20oC102 µl/ml5010010 µl/ml20 µl/ml

* - Unstable solution, prepare just before use;
** - Light sensitive;
*** - Light sensitive, Mg²⁺ - inhibitor;

Preparation of stock solutions.

filter sterilize; or prepare from the sterile water and the sterile powder;
store at -20^oC in small aliquots (avoid more than 10 freezing-thawing cycles);
it is possible to use 50% glycerol instead of H₂O. The advantage - solution is liquid at -20^oC.
some laboratories use 96% (or 50%) EtOH for ampicillin solubilization. There are two advantages in this method: (i) solution is liquid at -20^oC, (ii) the solution is self sterilizing. But the disadvantage is very serious: ethanol is a weak catalyst of ß-lactam ring disintegration. So, solution is unstable.

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