用T-wave离子淌度质谱鉴定猪肌肉中多位点分子离子(六)
结论
基于对本研究中氟喹诺酮化合物的特征离子化的观察,使用UPLC IMS MSE进行方法开发是合理的。
■ 使用离子淌度,可将同一分子,不同质子化物质实现神奇的分离。
■ 显示并鉴别了同分子,不同位点质子化合物的碎片谱图。
■ 得到每个组分的母离子MS及其MSE碎片谱图
■ HDMSE观察结果可以解释在同一实验室内监测特定MRM离子队获得的研究对象报告结果有时 会出现差异的原因。
■ 漂移时间值可作为保留时间、母离子精确质量数和碎片精确质量数谱图之外的又一个鉴定条件。
■ 离子淌度分离可以有效地将目标峰从基质干扰中分离出来,而不需要对复杂样品进行纯化及色谱分离。
参考文献
1. Final Decision of the Commissioner,
Docket # 2000N-I 57 1 October 24, 2000, Withdrawal of the approval of
the new animal drug application for enrofloxacin in poultry. Department
of Health and Human Services, U.S. Food and Drug Administration.
2.
FDA Center for Veterinary Medicine, June 2, 1997, FDA Order Prohibits
Extralabel Use Of Fluoroquinolones And Glycopeptides.
3. Official
Journal of the European Union, L268:29-43, Regulation (EC) No 1831/2003
of the European Parliament and of the Council of 22 September 2003 on
additives for use in animal nutrition.
4. Official Journal of the
European Union, L24:1-8 Annex I, Council Regulation (EEC) No 2377/90 of
26 June 1990 laying down a Community procedure for the establishment
of maximum residue limits of veterinary medicinal products in
foodstuffs of animal origin.
5. Verdon E, Couedor P, Roudaut B,
Sandérs PJ. Multiresidue Method for Simultaneous Determination of Ten
Quinolone Antibacterial Residues in Multimatrix/Multispecies Animal
Tissues by Liquid Chromatography with Fluorescence Detection: Single
Laboratory Validation Study. AOAC Inter. 2005; 88:1179-1192.
6.
Kaufmann A, Butcher P, Maden K, Widmer M, Giles K, Uria D. Are liquid
chromatography/electrospray tandem quadrupole fragmentation ratios
unequivocal confirmation criteria? Rapid Commun. Mass Spectrom. 2009;
23: 985-998.
7. Mol HG, Zomer P, de Koning M. Qualitative aspects
and validation of a screening method for pesticides in vegetables and
fruits based on liquid chromatography coupled to full scan high
resolution (Orbitrap) mass spectrometry. Anal Bioanal Chem. 2012;
403:2891-2908.
8. Croley TR, White KD, Callahan JH, Musser SM. T he
chromatographic role in high resolution mass spectrometry for
non-targeted analysis. J Am Soc Mass Spectrom. 2012; 23:1569.
9.
Commission Decision (2002/657EEC) Official Journal of the European
Communities 2002. 10. Lalli PM, Iglesias BA, Toma HE, de Sá GF, Daroda
RJ, Silva Filho JC, Szulejko JE, Araki K, Eberlin MN. Protomers:
formation, separation and characterization via travelling wave ion
mobility mass spectrometry. J Mass Spectrom. 2012; 47(6):712-9. 11.
Eberlin MN, Lali PM, Nachtigall FM, Riccio MF, de Sa GF, Daroda RJ, de
Souza V, Campuzano I, Souza GHMF. SYNAPT HDMS: Improving Ion Mobility
Separation by Increasing Drift-Gas Polarizability. Waters Technical
Note 720003201en. 2009 October. 12. Jurneczko E, Kalapothakis J,
Campuzano IDG, Morris M, Barran PE. Effects of drift gas on collision
cross sections of a protein standard in linear drift tube and
traveling wave ion mobility mass spectrometry. Anal Chem. 2012;
84(20):8524-31. 13.Asbury GR, Hill HH. Using different drift gases to
change separation factors (alpha) in ion mobility spectrometry. Anal.
Chem. 2000; 72(3): 580-4. 14. Fasciotti M, Sanvido GB, Santos VG, Lalli
PM, McCullagh M, de Sá GF, Daroda RJ, Petera MG, Eberlina MN. J Mass
Spectrom. 2012; 47(12):1643-7.