细菌人工染色体
The Construction of Bacterial Artificial Chromosome (BAC) Libraries (complete manuscript) (Clemson University Genomics Institute)
Construction of BAC Library (Caltech)
Guideline for BAC library preparation, the followings are described in great detailPreparation of BAC vector DNA
Preparation of source DNA
Ligation and Electroporation
Preparation of BAC DNA
BAC Manual (Texas A&M BAC Center)
Provide details in the theory and practice for the construction, characterization, and screening of BAC libraries.Hybridization of High Density Arrayed BAC Nylon Filter Blots (Caltech)
Procedures for isolation and purification of cDNA inserts using polymerase chain reaction, probe labeling, probe hybridization and more...
BAC/PAC/Fosmid Prep(WUGSC)
BAC End-Sequencing (Caltech)
Prepare BAC DNA for sequencing