Lactobacillus culture
Overview
General overview and guidelines on how to grow up a culture of Lactobacillus
Materials
MRS broth (difco)
MRS agar (difco)
anaerobic conditions
Procedure
Follow instructions to make MRS plates and media, autoclave to sterilize
Streak Lactobacillus on MRS plate or inoculate into test tube of MRS media
Incubate at 37C under anaerobic conditions
If from new source of Lactobacillus (ex. dehydrated bacteria from ATCC) allow up to 3 days to grow. Otherwise, allow 12-24 hours to grow
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