微电极阵列（Med-64）对野生型(WT)和W/W（v）型小鼠肠起...
微电极阵列（Med-64）对野生型(WT)和W/W（v）型小鼠肠起搏器活动的研究
Cajal间质细胞中的肌间神经丛区域（ICC-MyP）能形成一个网络，并产生基底起搏电活动。这种形态特征使我们相信，这些细胞对胃肠道协调运动是非常重要的。我们希望找出一种新的方法来对ICC-MyP的电场活动和网络功能作出评估。约1毫米面积的ICC-MyP 区域的场电位通过极距为150um的8x8微电极阵列进行测量, 含硝苯吡啶和河豚毒素的细胞外液能抑制平滑肌细胞和神经元。我们也比较了野生型（WT）和W/W（v）型小鼠回肠肌肉空间电场活动。通过3维时空分析法分析表明，WT型小鼠的基底电活动与传播延迟是同步进行的，而在W/W（v）型小鼠中同样信号幅度却较小和出现规律也没有那么正常的现象。WT型小鼠的功率谱中有一个突出的峰值，并与ICC-MyP的起搏器的活动频率对应，而W/W（v）型中是没有这个峰值的。因此，在9.4-27.0每千次的光谱功率中，WT野生型远远超过了W/W（v）型 。最后，微电极阵列测量表明，该网络不仅能产生，而且还调节基底电活动。由ICC网络形态和功能损伤所引起的胃肠运动失调将会在以后更加深入的研究中被发现。
Microelectrode array evaluation of gut pacemaker activity in wild-type and W/W(v) mice.
Nakayama S, Ohishi R, Sawamura K, Watanabe K, Hirose K.
Department of Cell Physiology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan. h44673a@nucc.cc.nagoya-u.ac.jp
Interstitial cells of Cajal in the myenteric plexus region (ICC-MyP) form a network and generate basal pacemaking electrical activity. This morphological feature leads us to believe that these cells may be essential for the coordinating actions of gastrointestinal (GI) motility. We aim to propose a new method for functional assessment of ICC electrical activity and its network. Field potentials in a approximately 1 mm(2) region were simultaneously measured using an 8x8 microelectrode array (MEA) with a polar distance of 150 microm. The extracellular solution contained nifedipine and tetrodotoxin (TTX) to suppress activities of smooth muscle cells and neurons, respectively. We compared spatial electrical activities between ileal muscle preparations from wild-type (WT) and W/W(v) mice. In spatio-temporal analyses, basal electrical activities were well synchronized with a propagation delay in WT, while those in W/W(v) were small in amplitude and irregular in occurrence. The power spectrum in WT had a prominent peak corresponding to the frequency of ICC-MyP pacemaker activity, while that of W/W(v) lacked it. Consequently, the ratio of the spectral power in 9.4-27.0 cpm was significantly larger in WT than in W/W(v). In conclusion, MEA measurements demonstrated that the network-forming ICC-MyP not only generates but also coordinates basal electrical activities. Disorders of GI motility based on morphological and functional impairments of ICC network with the range of several hundreds of micrometers, could be uncovered in future extensive studies.
文章来源：
http://www.ncbi.nlm.nih.gov/pubmed/19576758?ordinalpos=1&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_
DefaultReportPanel.Pubmed_RVDocSum
