Solutions for Sea Urchin Development

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2019.8.18

ASW: Artificial sea water (MBL)

NaCl24.72 gKCl0.67 gCaCl2.2H2O1.36 gMgCl2.6H2O4.66 gMgSO4.7H2O6.29 gNaHCO30.18 gdH2Oto 1 liter

Sulfate-free Sea water

NaCl27.72 gKCl0.67 gCaCl2.2H2O1.36 gMgCl29.32 gNaHCO30.18 gdH2Oto 1 liter

Development

0.5 M KCl to induce gamete shedding

3.73 g KCl/ 100 ml dH₂O

LSSW: Low sodium sea water (50 mM)

NaHCO30.2 gCholine Cl28.3 gKCl0.7 5gCaCl2.2H2O1.6 gMgCl2.6H2O5.5 gMgSO4.7H2O7.1 gdH2Oto 1 liter, adjust pH to 7.8

CaFSW: Calcium-free Seawater

NaCl25.6 gKCl0.7 gMgSO4.7H2O11.9 gNaHCO30.5 gdH2Oto 1 liter

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