肠炎造模：DSS在诱导非啮齿类动物肠炎模型的作用-3
4. 猪—Swine
背景介绍
分别将约克夏猪和西藏猪的肠道菌群移植入健康的新生仔猪中，建立「干预」模型。由 DSS
诱导成急性肠炎，激活了植入约克夏猪肠道菌群仔猪的结肠组织中的 TLR/NLR
信号通路，造成促炎性细胞因子和免疫细胞增加，结肠损伤。而采用西藏猪肠道菌群干预的仔猪并没有显著的炎症反应及细胞因子的增加。
实验概述
实验目的：用 DSS 分别诱导移植了约克夏猪菌群和西藏猪菌群的仔猪，建立急性肠炎模型。实验动物：新生的杂交商品型仔猪，遗传背景更接近约克夏猪。实验材料：DSS（36K-50KDa，MP Biomedicals）。
实验步骤
1. 将出生即与母猪分离的仔猪，分成 5 组（G1-G5），每组 6 只；
2. G1 组和 G2 组分别被移植约克夏猪的粪便悬液，G3 组和 G4 组被移入西藏猪的粪便悬液，G5 组（对照组）则被移植无粪便的无菌 PBS；
3. 头 15 天喂养牛奶替代品，然后换成固体商业食品，直到实验结束；
4. 喂养第 50 天，G2 组和 G4 组进行急性肠炎诱导，连续 5 天灌注含 4% DSS 的 100 mL 水。
实验结论
1. 灌注 DSS 后，移植了「约克夏猪肠道菌群」仔猪出现了肉眼可见的急性肠炎症状。诱导第 3，4，5 天，DAI 指数评分显著高于移植了「西藏猪肠道菌群」的仔猪（Fig. A）。
2. 结肠形态（Fig. B）和组织病理切片（Fig. C）结果表明，「约克夏猪肠道菌群」组的仔猪出现了严重的出血。
案例文献：Leonardi I, Nicholls F, Atrott K, Cee A, Tewes B, Greinwald R, Rogler G, Frey-Wagner I.Oral administration of dextran sodium sulphate induces a caecum-localized colitis in rabbits.Int J Exp Pathol. 2015 Jun;96(3):151-62.
【订购信息】
MP DSS 产品信息 MP Biomedicals 生产的 DSS 具有高质量、高纯度、高重复性的特点，迄今为止，有超过 5000 篇科学文献引用，有效性得到了业界广泛认可。
MP DSS 诱导非啮齿类动物肠炎模型部分文献
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兔
•Leonardi I, Nicholls F, Atrott K, Cee A, Tewes B, Greinwald R, Rogler G, Frey-Wagner I. Oral administration of dextran sodium sulphate induces a caecum-localized colitis in rabbits. Int J Exp Pathol. 2015 Jun;96(3):151-62.
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