原位PCR
About in situ PCR (Applied Biosystems)
Basic information about in situ PCR and its applications.The In Situ PCR: Amplification and Detection in a Cellular Context (Ernest F. Retzel, et al)
General guide and protocol for in situ PCR technique
In Situ PCR Cookbook (Katherine A. Staskus)
Detailed protocol for in situ PCR on tissue section
· In Situ PCR (Lazo Lab)
In situ PCR amplification on fixed tissue or cells, then Detection of Amplified DNA by in situ Hybridization
· In Situ PCR on Plant Materials (Johansen)
Detailed protocol on tissue preparation, treatment, transcription, detection and more...
In Situ RT-PCR
· RT In Situ PCR (Gerard J. Nuovo)
RT in situ PCR allows for the routine and rapid detection of low copy viral and human RNAs. Success with RT in situPCR is best accomplished with formalin fixed, paraffin embedded material, which allows the study of archival material.
· In Situ PCR On Plant Tissues (Bo Johansen)
Provides detailed protocol on tissue preparation, in situ PCR, detection and required reagents.