JC-1分析线粒体膜电位的方法-3

3.6 Key references

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Appendix 1: Stock solution:

JC-1 is dissolved in N,N’-dimethylformamide (Sigma-Aldrich, cat. n. D8654) at the concentration of 2.5 mg/ml.

It is stored at -20°C. Light sensitive.

Appendix 2: Reagents

Note: colture medium, saline solutions and washing buffers are depending on the cell type which is used for the experimental procedure (PBMC, fibroblasts, hepatocytes, etc.). For blood white cells, RPMI 1640 with 10% heat inactivated foetal calf serum, 100 IU/ml penicillin, 100 mg/ml streptomycin, 2 mM L-glutamine is normally used as complete colture medium.

Appendix 3: Equipment

Appendix 4: Glossary

Mitochondrial membrane potential (Dy) is generated by mitochondrial electron transport chain, which drives a proton flow from matrix through inner mitochondrial membrane to cytoplasm, thus creating an electrochemical gradient. This gradient is in turn responsible for the formation of ATP molecules by F₀-F₁ ATP synthase. For this reason Dy is an important parameter for mitochondrial functionality and an indirect evidence of energy status of the cell.