蛋白质标记
Biosynthetic labeling (Sefton Lab)
Biotinylation of Antibody (Contributed by Nanci Donacki)
125I Labeling of Protein using ICl (ScienceXchange)
Protein (Antibody) Fluorescent Labeling (Mario Roederer)
Gives detailed protocol for conjugation of proteins to fluorescent dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation of non-immunoglobulin proteins like Annexin V). Procedures for the conjugation of immunoglobulins are provided for the following molecules:
In addition, protocols for conjugation of Annexin V to the following molecules are given as models for fluorescent conjugation of non-immunoglobulin proteins.