Blue Native Gel Electrophoresis
Blue Native Gel Electrophoresis
Stock solutions
49.5%T, 3%C Acrylamide 24 g acrylamide, 0.75 g bisacrylamide / 50 ml H2O Store at RT
3 x Gel buffer 150 mM BisTris-HCl, 1.5 M 6-amino-caproic acid, pH 7.0 Adjust pH to 7.0 with HCl at 4°C Store at 4°C
75% (w/v) Glycerol Store at 4°C
10 x Cathode buffer 0.5 M Tricine, 150 mM BisTris No need to adjust pH Store at 4°C
5 x Anode buffer 0.25 M BisTris-HCl, pH 7.0 Adjust pH to 7.0 with HCl at 4°C Store at 4°C
2 x BisTrisACA 200 mM BisTris-HCl, 1 M 6-amino-caproic acid, pH 7.0 Adjust pH to 7.0 with HCl at 4°C Store at 4°C
50BTH40G 50 mM BisTris-HCl, 40% (w/v) Glycerol, pH 7.0 Adjust pH to 7.0 with HCl at 4°C Store at 4°C
Working solutions
1xCathode (-dye), 1x Anode buffer Store at 4°C
1xCathode+dye 0.01% Serva G in 1xCathode buffer
It takes a while to dissolve ServaG. Store at 4°C
BN-sample buffer
Serva G
50 mg
2 x BisTrisACA
500 ul
75% Sucrose
400 ul
H2O
100 ul
Store at -20°C
Solubilization buffer
50BTH40G
100 ul
10% Detergent
40 ul
H2O
60 ul
