Skeletal Muscle: Myogenesis & Myopathy

PCR Array

骨骼肌：成肌及肌病PCR基因芯片
 

Product	Species	Technology	Cat. No.
Skeletal Muscle: Myogenesis & Myopathy PCR Array	Human	Gene Expression	PAHS-099Z
Skeletal Muscle: Myogenesis & Myopathy PCR Array	Mouse	Gene Expression	PAMM-099Z
Skeletal Muscle: Myogenesis & Myopathy PCR Array	Rat	Gene Expression	PARN-099Z
Skeletal Muscle: Myogenesis & Myopathy PCR Array	Rhesus Macaque	Gene Expression	PAQQ-099Z

    Skeletal Muscle Development & Disease RT² Profiler™ PCR Array profiles the expression of 84 key genes involved in skeletal muscle differentiation, function and disease-related processes. Skeletal muscle’s role in voluntary movement contributes greatly to energy metabolism and its regulation via glucose uptake and storage by insulin. Complications from aging and metabolic diseases like diabetes and metabolic syndrome contribute to muscle wasting (atrophy). However, recent research hypothesizes that metabolic defects in skeletal muscle contribute to the etiology of diabetes and metabolic syndrome, suggesting that skeletal muscle has a larger role in these disease states than initially expected. Large heterogeneous protein complexes including titin or dystrophin facilitate muscle contraction by connecting the skeletal muscle cytoskeleton to the extracellular matrix. Muscular dystrophies arise from inherited mutations in the genes encoding components of these complexes, and gene expression changes disrupting their normal contractile function dysregulate signaling pathways that control muscle growth. Potential therapies for muscle wasting include generation of new muscle cells (myogenesis) or increasing the mass of current muscle cells (hypertrophy). Thus, muscle-specific biological and pathophysiological processes are interrelated and cannot be studied in isolation. This array includes genes important for basic skeletal muscle function, development and growth, as well as genes related to the disease processes of metabolic syndrome and muscle wasting. Using real-time PCR, you can easily and reliably analyze the expression of a focused panel of genes involved in skeletal muscle development and disease with this array.
    骨骼肌：成肌及肌病PCR基因芯片可以同时检测与骨骼肌分化、功能与疾病相关的84个关键基因的表达。骨骼肌的随意运动，极大地促进能量代谢和胰岛素调节葡萄糖的摄取和存储。老化及代谢性疾病，如糖尿病和代谢综合征的并发症会导致肌肉萎缩。然而，最近的研究推测骨骼肌的代谢缺陷有助于糖尿病和代谢综合征的发病，表明在这些疾病状态中骨骼肌比最初预期有更大的作用。巨型的多源蛋白复合体，例如titin或dystrophin，会促进骨骼肌细胞的细胞骨架连接到细胞外基质上，并引起肌肉收缩。这些复合物组成蛋白的遗传性突变和相关基因表达的变化都会引起肌营养不良症。治疗肌肉萎缩的可行性方案包括促进生成新的肌肉细胞（成肌）或提升肌肉细胞的质量。该芯片包括参与骨骼肌基本功能，发展和增长的重要基因，及与代谢综合征和肌肉萎缩的疾病相关的基因。利用实时定量PCR，研究者可以方便并且可信地对骨骼肌肉的发育和疾病有关基因进行同时检测。
Skeletal Muscle Contractility:
Dystrophin-Glycoprotein Complex:CAMK2G, CAPN3, CAV3, DAG1, DMD (Dystrophin), DYSF, LMNA, MAPK1, SGCA.
Titin Complex:ACTA1, ACTN3, CAPN3, CRYAB, DES, LMNA, MAPK1, MSTN, MYH1, MYH2, MYOT, NEB, SGCA, TNNI2, TNNT1, TNNT3, TRIM63 (MuRF1), TTN. 
Energy Metabolism:CS, HK2, PDK4, SLC2A4 (GLUT4). 
Fast-Twitch Fibers:ATP2A1, MYH1, MYH2, TNNI2, TNNT3.
Slow-Twitch Fibers:MB, MYH1, TNNC1, TNNT1.
Other:DMPK, IKBKB, RPS6KB1.
Skeletal Myogenesis:ACTA1, ADRB2, AGRN, BCL2, BMP4, CAPN2, CAST, CAV1, CTNNB1, DMD, HDAC5, IGF1, IGFBP3, IGFBP5, MEF2C, MSTN, MUSK, MYF5, MYF6, MYOD1, MYOG, PAX3, PAX7, PPP3CA (Calcineurin Aa), RHOA, RPS6KB1, UTRN.
Skeletal Muscle Hypertrophy:ACTA1, ACVR2B, ADRB2, IGF1, IGFBP5, MSTN, MYF6, MYOD1, RPS6KB1.
Skeletal Muscle Autocrine Signaling:ADIPOQ, FGF2, IGF1, IGF2, IL6, LEP, MSTN, TGFB1.
Diabetes/Metabolic Syndrome:ADIPOQ, LEP, PPARG, PPARGC1A (PGC-1a), PPARGC1B (PGC-1ß), PRKAA1(AMPK), PRKAB2, PRKAG1, PRKAG3, SLC2A4 (GLUT4).
Skeletal Muscle Wasting/Atrophy:
Autophagy:CAPN2, CASP3, FBXO32 (Atrogin-1), FOXO1, FOXO3, MSTN, NOS2, PPARGC1A (PGC-1a),
PPARGC1B (PGC-1ß), RPS6KB1, TRIM63 (MuRF1).
Dystrophy:AKT1, AKT2, FBXO32 (Atrogin-1), IL1B, MAPK1, MAPK14, MAPK3, MAPK8, MMP9, NFKB1, TNF,
TRIM63 (MuRF1), UTRN.