Cancer Inflammation & Immunity Crosstalk PCR Array

人癌症炎症反应和免疫交互作用PCR芯片 

PCR Array   Product Species Technology Cat. No. Cancer Inflammation & Immunity Crosstalk PCR Array Human Gene Expression PAHS-181Z Cancer Inflammation & Immunity Crosstalk PCR Array Mouse Gene Expression PAMM-181Z Cancer Inflammation & Immunity Crosstalk PCR Array Rat Gene Expression PARN-181Z

The Human Cancer Inflammation & Immunity Crosstalk RT² Profiler PCR Array profiles the expression of 84 key genes involved in mediating communication between tumor cells and the cellular mediators of inflammation and immunity. In addition to epithelial and stromal compartments, the tumor microenvironment contains several cell types of the innate and adaptive immune systems including B and T lymphocytes, dendritic cells, and macrophages. In response to tumor-associated antigens presented via MHC Class I molecules, or to abnormal molecular patterns recognized by Toll-like receptors, the immune system eliminates target cells using a variety of effector enzymes and the engagement of pro-apoptotic signals including TRAIL and FAS ligand. If normal homeostasis is not resolved quickly, a state of chronic inflammation can ensue, including locally increased levels of reactive oxygen and nitrogen species that promote genomic instability. Immune cells produce a variety of cytokines that coordinate the inflammatory response, which is fueled by positive feedback loops commonly involving the STAT and NFκB signaling pathways in tumor cells. The resulting upregulation of antiapoptotic and immunosuppressive factors enables transformed cells to proliferate unchecked by the immune system. During cancer progression, the repertoire of chemokines, cytokines, and growth factors that orchestrates normal immune responses can be commandeered to create an immunosuppressive state that facilitates invasion and metastasis. The genes profiled with this array include mediators and effectors of the cross-talk between tumors and the immune system that influences the course of cancer progression. A set of controls present on each array enables data analysis using the ΔΔC_T method of relative quantification as well as assessment of reverse transcription performance, genomic DNA contamination, and PCR performance. Using real-time PCR, researchers can easily and reliably analyze the expression of a focused panel of genes involved in cancer inflammation and immune crosstalk with this array.
 
人癌症炎症反应和免疫交互作用PCR芯片研究参与调节炎症反应和免疫过程中肿瘤细胞和细胞介质之间的通讯的84关键基因的表达。除了上皮和基质隔间，肿瘤微环境包含几个先天和适应性免疫系统的细胞类型包括B和T淋巴细胞、树突状细胞和巨噬细胞。通过MHC I型分子应答肿瘤相关抗原呈现或toll样受体识别异常分子模式中,免疫系统使用各种效应酶和参与促凋亡信号的衔接包括TRAIL和FAS配体消除靶细胞。如果不迅速解决正常的体内平衡，慢性炎症状态就会接踵而至,包括原地提高活性氧水平和氮种类促进基因组不稳定。免疫细胞产生多种细胞因子协调炎性反应 ,被正反馈循环刺激通常涉及在肿瘤细胞中的STAT和NFκB信号通路。上调抗凋亡和免疫抑制因子结果使转化细胞增殖不被免疫系统检测。癌症恶化期,趋化因子组分,细胞因子和协调正常免疫反应的生长因子,可被招募来创建一个免疫抑制状态,促进入侵和转移。这个芯片的基因包括肿瘤和影响癌症恶化进程的免疫系统之间交互作用的介质和效应器。每张芯片含一个对照组使得分析数据时可以用相对定量ΔΔCT方法评估逆转录效率,基因组DNA污染,和PCR效率。利用这张芯片，通过实时定量PCR，可以简易且可靠地分析癌症炎症和免疫反应交互作用的关键基因的表达。
PCR芯片仅用于分子生物学应用。本产品不用于疾病的诊断、预防和治疗。
提供96孔板——384 -(4 x 96)板,和100孔板
 
Immune & Inflammatory Responses:
Immunostimulatory Factors:IFNG, IL2, IL12A, IL12B, IL15, TNF.
Immunosuppressive Factors:CD274 (PD-L1), CSF2 (GM-CSF), CTLA4, CXCL12 (SDF1), CXCL5, IDO1 (IDO), IL10, IL13, IL4, IL8, MIF, NOS2 (iNOS), PDCD1 (PD1), PTGS2 (COX2), TGFB1, VEGFA.
Pro-Inflammatory Genes:CCL2 (MCP-1), CCL20 (MIP-3A), IFNG, IL1A, IL1B, IL2, IL6, IL12A, IL12B, IL17A, IL23A, PTGS2 (COX2), TLR4, TNF, VEGFA.
Anti-Inflammatory Genes:IL4, IL10, IL13, TGFB1.
Enzymatic Modulators of Inflammation & Immunity:AICDA (AID), GZMA, GZMB, IDO1 (IDO), NOS2 (iNOS), PTGS2 (COX2).
Antigen Presentation:HLA-A, HLA-B, HLA-C, MICA, MICB.
Chemokines:CCL2 (MCP-1), CCL4 (MIP-1B), CCL5 (RANTES), CCL18 (PARC), CCL20 (MIP-3A), CCL21, CCL22 (MDC), CCL28, CXCL1, CXCL2, CXCL5, CXCL9 (MIG), CXCL10 (IP-10), CXCL11 (I-TAC, IP-9), CXCL12 (SDF1).
Chemokine Receptors:ACKR3 (CXCR7), CCR1, CCR2, CCR4, CCR7, CCR9, CCR10, CXCR1 (IL8RA), CXCR2 (IL8RB), CXCR3, CXCR4, CXCR5.
Interleukins:IL1A, IL1B, IL2, IL4, IL6, IL8, IL10, IL12A, IL12B, IL13, IL15, IL17A, IL23A.
Other Cytokines:KITLG (SCF), MIF, SPP1, TNF, TNFSF10 (TRAIL).
Growth Factors & Receptors:CSF1 (MCSF), CSF2 (GM-CSF), CSF3 (GCSF), EGF, EGFR, IGF1, TGFB1, VEGFA.
Signal Transduction:
Interferon Signaling:GBP1, IFNG, IL6, IRF1.
Interferon-Responsive Genes:CCL2 (MCP-1), CCL5 (RANTES), CXCL9 (MIG), CXCL10 (IP-10), GBP1, IRF1, MYD88, STAT1, TLR3, TNFSF10 (TRAIL).
NFκB Targets:BCL2L1 (BCL-XL), CCL2 (MCP-1), CCL5 (RANTES), CSF1 (MCSF), CSF2 (GM-CSF), CSF3 (GCSF), IFNG, IL8, TNF.
STAT Targets:CCL2 (MCP-1), CCL4 (MIP-1B), CCL5 (RANTES), CSF1 (MCSF), CSF2 (GM-CSF), CSF3 (GCSF), CXCL9 (MIG), CXCL10 (IP-10), CXCL11 (I-TAC, IP-9), CXCL12 (SDF1), IL1B, IL6, IL8, IL10, IL17A, IL23A, MYC.
Toll-Like Receptor Signaling:TLR2, TLR3, TLR4, MYD88.
Transcription Factors:FOXP3, HIF1A, IRF1, MYC, NFKB1, STAT1, STAT3, TP53 (p53).