人Notch信号通路增强版PCR芯片可以同时检测与Notch信号通路相关的84个基因表达。同时测定实验组样本中经典的Notch信号通路活性是被激活，抑制还是没有变化。此芯片中包括Notch通路配体、受体及其相关基因，也包含公认的Notch靶基因，还包含细胸凋亡、细胞周期、神经发生和转录调控相关基因，和参与细胞调控、增殖和分化的基因。该芯片还包括与Notch信号通路有交叉的其他信号通路的基因，如Sonic Hedgehog和Wnt Receptor信号通路成员基因。该芯片同时包含了16个通过实验验证得到的显著标志基因，通过分级运算程序可计算得到活性分值。每块芯片上都设定了一组对照通过ΔΔCT方法分别对反转效率，基因组DNA含量和PCR效率进行评估。通过实时定量PCR的方法，研究者即能够利用该芯片简单可靠地同时检测Notch信号通路相关基因的表达。
Notch Signaling Pathway: Notch Ligands: NOTCH1, NOTCH2, NOTCH3, NOTCH4. Notch Binding: DLL1, DLL3, DLL4, DTX1, JAG1, JAG2, LFNG, MFNG, NUMB, RFNG. Notch Receptor Processing: ADAM10, ADAM17 (CD156B), NCSTN, PSEN1, PSEN2, PSENEN (PEN2). Transcription Factors & Cofactors: EP300, HDAC1, MAML1, MAML2, NCOR2, RBPJL, SNW1 (SKIIP). Notch Target Genes: Apoptosis: CDKN1A (p21CIP1/WAF1), CFLAR (CASPER), FOSL1 (FRA1), ID1, IL2RA (CD25), NFKB1, PTCRA. Cell Cycle: CCND1, CDKN1A (p21CIP1/WAF1). Cell Adhesion: CD44, ERBB2 (HER2). Cell Differentiation & Development: DTX1, HES1, HES5, HEY1, HEY2, HEYL, JAG1, KRT1, LFNG, LOR, NOTCH1, PPARG. Neurogenesis: ERBB2 (HER2), FOS, HES1, HEY1. Immune Response: CHUK (IKKa), IFNG, IL2RA (CD25), NFKB1, NFKB2, STAT6. Transcription Factors: FOS, FOSL1 (FRA1), HES1, HES5, HEY1, HEY2, HEYL, ID1, NFKB1, NFKB2, PPARG, STAT6. Other Genes Involved in the Notch Signaling Pathway: Apoptosis: AXIN1, CTNNB1. Cell Cycle: AXIN1, CCNE1, FIGF (VEGFD), JAG2, NOTCH2. Cell Proliferation: FIGF, LRP5. Cell Differentiation: PAX5, SHH. Neurogenesis: PAX5. Transcription Factors & Cofactors: AES (Groucho), CBL, CTNNB1, GLI1, HOXB4, HR, PAX5, POFUT1, RUNX1 (AML1), TLE1. Other Genes: LMO2, MMP7, SEL1L. Pathways that Crosstalk with the Notch Signaling Pathway: Hedgehog Signaling: GLI1, GSK3B, SHH, SMO. WNT Signaling: AES (Groucho), AXIN1, CTNNB1, FZD7, GSK3B, LRP5, TLE1, WISP1. Pathway Activity Signature Genes: ADA, AFAP1L2, DTX1, H19, HES4, HES5, HEY2, HEYL, NRARP, SCGB1A1, SERPINA3, SLC6A12, SNAI2, TFF1, TNFSF10, UBD.
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