ASTM D5712-2005e1
用改进半空细胞法分析天然橡胶及其制品中水可萃取蛋白质的标准试验方法

Standard Test Method for Analysis of Aqueous Extractable Protein in Natural Rubber and Its Products Using the Modified Lowry Method

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标准号

ASTM D5712-2005e1

发布日期

2005年

实施日期

废止日期

无

中国标准分类号

G40

国际标准分类号

83.040.10 (Latex and raw rubber)

发布单位

美国材料与试验协会

适用范围

This test method, for the determination of protein levels in NR, is primarily intended to test NR materials for residual protein content. It is assumed that all who use this test method will be trained analysts capable of performing common laboratory procedures skillfully and safely. It is expected that work will be performed in a properly equipped laboratory.

1.1 This test method covers an analytical test for determining the amount of total aqueous extractable protein associated with natural rubber (NR) and its products. Water soluble proteins are extracted in a buffer solution and then precipitated to concentrate them and also to separate them from water soluble substances that may interfere with the determination. The extracted protein is redissolved and quantified colorimetrically by the modified Lowry method using a protein standard.

1.2 For the purpose of this test method, the range of protein measurement will be based on the limit of detection and quantitation and recorded in micrograms per dm² NR specimen.

1.3 The test method is designed to be accurate and compatible with the industrial environment.

1.4 Steps are included in this test method to minimize the effects of interfering substances.

1.5 It is recognized that other methods for the analysis of leachable proteins exist and these may be used for routine quality control purposes provided they have been validated and a correlation established against the reference method specified by this test method.

1.6 The values stated inSI units are to be regarded as the standard. No other units of measurement are included in this standard.

1.7 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.

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