ASTM E579-04(2015)
溶液中硫酸奎宁的荧光检测限值的标准试验方法

Standard Test Method for Limit of Detection of Fluorescence of Quinine Sulfate in Solution

被代替

标准号

ASTM E579-04(2015)

发布

2004年

发布单位

美国材料与试验协会

替代标准

ASTM E579-04(2023)

当前最新

ASTM E579-04(2023)

 

 

引用标准

ASTM E578

适用范围

4.1 When determining the limiting detectable concentration of a fluorescent substance, it is usually necessary to increase the readout scale of a photoelectric instrument to a point where noise (that is, random fluctuations of the system) becomes apparent. This noise will be superimposed upon the signal from the sample.

4.2 In molecular fluorescence spectroscopy, the limit of detection for the sample will be determined by the limiting signal-to-noise ratio, S/N, where the signal, S, is the difference between readings obtained with the sample and blank solutions, and N is the total root-mean-square (rms) noise. The limit of detection for the sample will be given by the instrument readings that give a signal equal to three times the rms value of the noise.

Note 2: Factors other than noise affecting the sample concentration corresponding to the limit of detection include: the spectral bandwidths of the excitation and emission monochromators, the intensity of the exciting light that can be concentrated on the sample, the fraction of the fluorescence collected by the detection system, the response time of the detection system, and the purity of the solvent. The size and arrangement of the sample container with respect to the light beams are also important, as they affect both the desired signal and the extraneous signal that only contributes noise.

Note 3: The value of rms noise (N) can be obtained by calculating the standard deviation of a series of readings of the signal from the sample at the peak emission wavelength at approximately 450 nm as follows:

where:
x	=	mean of the series of readings,
x	=	value of the individual reading, and
n	=	number of readings.

Alternatively, rms noise may be estimated by noting the extreme differences between the members of a series of readings (peak-to-peak noise) and dividing by a factor that is usually taken to be 5.6^,8201; ......

ASTM E579-04(2015)相似标准

推荐

硫酸 测试方法

/

AASHTO - American Association of State Highway and Transportation Officials

AENOR

ANSI - American National Standards Institute

API - American Petroleum Institute

AR-IRAM

ASD-STAN - Aerospace and Defence Industries Association of Europe - Standardization

AT-ON