07.100.01 微生物学综合 标准查询与下载

ASTM E2799-11 用 MBEC 化验测试消毒剂对绿脓杆菌生物膜的功效的标准试验方法

Vegetative biofilm bacteria are phenotypically different from suspended planktonic cells of the same genotype. Biofilm growth reactors are engineered to produce biofilms with specific characteristics. Altering either the engineered system or operating conditions will modify those characteristics. The goal in biofilm research and efficacy testing is to choose the growth reactor that generates the most relevant biofilm for the particular study. The purpose of this test method is to direct a user in how to grow, treat, sample and analyze a Pseudomonas aeruginosa biofilm using the MBEC Assay. Microscopically, the biofilm is sheet-like with few architectural details as seen in Harrison et al (5). The MBEC Assay was originally designed as a rapid and reproducible assay for evaluating biofilm susceptibility to antibiotics (2). The engineering design allows for the simultaneous evaluation of multiple test conditions, making it an efficient method for screening multiple disinfectants or multiple concentrations of the same disinfectant. Additional efficiency is added by including the neutralizer controls within the assay device. The small well volume is advantageous for testing expensive disinfectants, or when only small volumes of the disinfectant are available. 1.1 This test method specifies the operational parameters required to grow and treat a Pseudomonas aeruginosa biofilm in a high throughput screening assay known as the MBEC (trademarked) (Minimum Biofilm Eradication Concentration) Physiology and Genetics Assay. The assay device consists of a plastic lid with ninety-six (96) pegs and a corresponding receiver plate with ninety-six (96) individual wells that have a maximum 200 μL working volume. Biofilm is established on the pegs under batch conditions (that is, no flow of nutrients into or out of an individual well) with gentle mixing. The established biofilm is transferred to a new receiver plate for disinfectant efficacy testing. The reactor design allows for the simultaneous testing of multiple disinfectants or one disinfectant with multiple concentrations, and replicate samples, making the assay an efficient screening tool. 1.2 This test method defines the specific operational parameters necessary for growing a Pseudomonas aeruginosa biofilm, although the device is versatile and has been used for growing, evaluating and/or studying biofilms of different species as seen in Refs (1-4). 1.3 Validation of disinfectant neutralization is included as part of the assay. 1.4 This test method describes how to sample the biofilm and quantify viable cells. Biofilm population density is recorded as log colony forming units per surface area. Efficacy is reported as the log reduction of viable cells. 1.5 Basic microbiology training is required to perform this assay. 1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.7 ASTM International takes no position respecting the validity of any patent rights asserted in connection with any item mentioned in this standard. Users of this standard are expressly advised that determination of the validity of any such patent rights, and the risk of infringement of such rights, are entirely their own responsibility. 1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory......

Standard Test Method for Testing Disinfectant Efficacy against Pseudomonas aeruginosa Biofilm using the MBEC Assay

ASTM E2197-11 测定液体化学杀菌剂的杀菌,杀毒,杀真菌,杀分支杆菌和杀孢子的活性定量盘带菌者的标准试验方法

The design of this test eliminates any loss of viable organisms through wash off, thus making it possible to produce statistically valid data using many fewer test carriers than needed for methods based on simple MPN estimates. The stringency in the test is provided by the use of a soil load, the microtopography of the brushed stainless steel carrier surface, and the smaller ratio of test substance to surface area typical for many disinfectant applications. Thus, the test substance being assessed is presented with a reasonable challenge while allowing for efficient recovery of the test organisms from the inoculated carriers. The metal disks in the basic test are also compatible with a wide variety of actives. The design of the carriers makes it possible to place onto each a precisely measured volume of the test organism (10 μL) as well as the control fluid or test substance (50 μL). The inoculum is placed at the center of each disk whereas the volumes of the test substance covers nearly the entire disk surface, thus virtually eliminating the risk of any organisms remaining unexposed. In all tests, other than those against viruses, the addition of 10 mL of an eluent/diluent gives a 1:200 dilution of the test substance immediately at the end of the contact time. While this step in itself may be sufficient to arrest the microbicidal activity of most actives, the test protocol permits the addition of a specific neutralizer to the eluent/diluent, if required. Except for viruses, the membrane filtration step also allows processing of the entire eluate from the test carriers and, therefore, the capture and subsequent detection of even low numbers of viable organisms that may be present. Subsequent rinsing of the membrane filters with saline also reduces the risk of carrying any inhibitory residues over to the recovery medium. Validation of the process of neutralization of the test substance is required by challenge with low numbers of the test organism. In tests against viruses, addition of 1 mL of buffer at the end of the contact time achieves a 1:20 dilution of the test substance while keeping the volume of the eluate reasonably small to allow for the titration of most or all of the eluate in cell cultures. Confirmation of neutralization of the test substance is required by challenge of a residual disinfection load with low numbers of infective units of the test virus. Since the virus assay system is indirect, an additional step is required to demonstrate that prior exposure of the appropriate cell line to any residual disinfectant or disinfectant/neutralizer mixture does not interfere with the detection of a low level of virus challenge (See Appendix). Note 18212;In 5.5 and 5.6, volumes of 10 mL and 1 mL are recommended instead of 9.95 mL and 950 μL, respectively, for ease of dispensing the eluent. The soil load in this test is a mixture of three types of proteins (high molecular weight proteins, low molecular weight peptides, and mucous material) designed to represent body secretions, excretions, or other extraneous substances that microbicidal chemicals may encounter under field conditions. It is suitable for working with all types of test organisms included here. The components of the soil load are readily available and subject to much less variability than animal sera. If distilled water or other diluent is not to be specified on the product label, the diluent for the test substance is assumed to be tap water. Since the quality of tap water varies considerably both geographically and temporally, this test method incorporates the use of water with a specified and documented level of hardness to prepare use-dilutions of test substance that require dilution in water before use. While water with..........

Standard Quantitative Disk Carrier Test Method for Determining the Bactericidal, Virucidal, Fungicidal, Mycobactericidal and Sporicidal Activities of Liquid Chemical Germicides

KS J 0012-2010(2021) 二级（层流）生物安全橱柜

Class II(laminar flow) biosafety cabinetry

KS J 0012-2010(2015) II类（层流）生物安全厨柜

Class II(laminar flow) biosafety cabinetry

ASTM E2198-10 智利小植绥螨 Athias-Henriot的规范(蜱螨目:植绥螨科)

This method was developed to determine that the number of P. persimilis supplied in the shipment meets the package claim. The application of this method will ensure a standardized evaluation of the product and a judicious decision about product compliance to the package claim.1.1 This specification includes standard terminology, classification, and referenced documents as well as description of the test method determining whether the number of P. persimilis in the shipment and the purity of shipments meet the quantity specification. 1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 4.1 The test describes the method for determining if the number of the predatory mites conforms to the advertised standard and the method for assessment of purity of shipments.

Specification for Phytoseiulus persimilis Athias-Henriot (Acarina:Phytoseiidae)

LST ISO 21528-2:2009 食品和动物饲料的微生物学 肠杆菌科细菌的水平检测和计数方法 第2部分：菌落计数法（ISO 21528-2:2004，相同）

Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 2: Colony-count method (ISO 21528-2:2004, identical)

LST ISO 21528-1:2009 食品和动物饲料微生物学 肠杆菌科细菌检测和计数的水平方法 第1部分：采用预富集的 MPN 技术进行检测和计数（ISO 21528-1:2004，相同）

Microbiology of food and animal feeding stuffs - Horizontal methods for the detection and enumeration of Enterobacteriaceae - Part 1: Detection and enumeration by MPN technique with pre-enrichment (ISO 21528-1:2004, identical)

SANS 5614:2009 指間髮癬菌的抑制生长能力

Specifies a method for the determination of the resistance of test specimens to the growth of Trichophyton interdigitale.

Resistance to growth of Trichophyton interdigitale

JIS K 3702:2008 培养基的试验方法.用于微生物检验试验样品的初始悬浮和十倍稀释的准备

この規格は，微生物の検出を目的とする培地試験に用いる試料懸濁液及び希釈系列の調製方法について規定する。

Test methods for Culture media -- Preparation of initial suspension and decimal dilutions

JIS K 3701:2008 培养基.微生物检验的一般规则

この規格は，微生物の検出を目的とする培地試験の通則について規定する。

Culture media -- General rules for microbiological examinations

ASTM E2199-08e1 粉虱丽蚜小蜂Gahan的标准规范(膜翅目Hymenoptera:日光蜂科Aphelinidae)

This method was developed to determine that the numbers of E. formosa supplied in a shipment meet the package claim and that wasps at receipt have good flight capability. The application of this method will ensure a standardized evaluation of the product and judicious decisions about product compliance to the package claim. 1.1 This specification describes a method for determining whether the quantity and quality of adult Encarsia formosa in a shipment adhere to quantity and quality specifications. The test also allows the purity of shipments to be determined. Included are referenced documents, a description of standard terminology, specifications, and the test method.

Standard Specification for Encarsia formosa Gahan (Hymenoptera:Aphelinidae)

KS J ISO 8914-2007 微生物学副溶血弧菌检测通用指南

Microbiology－General guidance for the detection of Vibrio parahaemolyticus

KS J ISO 8914:2007 微生物学副溶血弧菌检测通用指南

Microbiology－General guidance for the detection of Vibrio parahaemolyticus

KS J ISO 7954-2007 微生物学酵母菌和霉菌计数通用指南25℃菌落计数技术

Microbiology－General guidance for enumeration of yeasts and moulds－Colony count technique at 25 ℃

KS J ISO 7954:2007 微生物学酵母菌和霉菌计数通用指南25℃菌落计数技术

Microbiology－General guidance for enumeration of yeasts and moulds－Colony count technique at 25 ℃

KS J ISO 21528-1-2007(2021) 食品和动物饲料微生物学肠杆菌科检测和计数的水平方法第1部分:预富集MPN技术检测和计数

Microbiology of food and animal feeding stuffs－Horizontal methods for the detection and enumeration of Enterobacteriaceae－Part 1：Detection and enumeration by MPN technique with pre-enrichment

KS J ISO 21528-1-2007(2012) 食品和动物饲料微生物学肠杆菌科检测和计数的水平方法第1部分:预富集MPN技术检测和计数

Microbiology of food and animal feeding stuffs－Horizontal methods for the detection and enumeration of Enterobacteriaceae－Part 1：Detection and enumeration by MPN technique with pre-enrichment

KS J ISO 21528-1:2007 食品和动物饲料的微生物学.肠杆菌科检测和计数的水平法.第1部分:通过预浓缩MPN技术进行计数和检测

이 규격은 1차 증균 후 장내세균 집단을 검출하기 위한 방법에 대하여 규정한다. 이것은 다

Microbiology of food and animal feeding stuffs－Horizontal methods for the detection and enumeration of Enterobacteriaceae－Part 1：Detection and enumeration by MPN technique with pre-enrichment

KS H ISO 8523-2007 微生物学 预富集肠杆菌科细菌检测通用指南

Microbiology－General guidance for the detection of Enterbacteriaceae with pre-enrichment

SANS 6133:2007 硫酸盐还原细菌的计数．最大可能数量（MPN）技术

Specifies the most probable number (MPN) technique for the enumeration of sulfate-reducing bacteria.

Enumeration of sulfate-reducing bacteria - Most probable number (MPN) technique