13.060.70 水的生物学特性的检验 标准查询与下载

EN ISO 19040-1:2022 水质.水和废水雌激素潜能的测定.第1部分：酵母雌激素筛选（酿酒酵母）

This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with genetically modified yeast strains Saccharomyces cerevisiae. This reporter gene assay is based on the activation of the human estrogen receptor alpha. This method is applicable to: — fresh water; — waste water; — aqueous extracts and leachates; — eluates of sediments (fresh water); — pore water; — aqueous solutions of single substances or of chemical mixtures; — drinking water. The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 8 ng/l and 15 ng/l 17β-estradiol equivalents (EEQ) based on the results of the international interlaboratory trial (see Annex F). The upper threshold of the dynamic range for this test is between 120 ng/l and 160 ng/l 17β-estradiol equivalents (EEQ). Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre-concentration of water samples can prove necessary, if their estrogenic potential is below the given LOQ.

Water quality - Determination of the estrogenic potential of water and waste water - Part 1: Yeast estrogen screen (Saccharomyces cerevisiae) (ISO 19040-1:2018)

T/HBFIA 0031-2022 水中菌落总数的测定 显色平板计数法

本文件描述了生活饮用水及其水源水中菌落总数的显色平板计数法

Chromogenic plate counting method for the determination of the total number of bacterial colonies in water

22/30430275 DC BS ISO 4979 水质 基于青萍根再生的水生毒性试验

BS ISO 4979. Water quality. Aquatic toxicity test based on root re-growth in Lemna minor

BS ISO 23196:2022 水质 生物等效性（BEQ）浓度的计算

What is this ISO 23196 - Biological equivalence (BEQ) concentrations about? ISO 23196 discusses water quality with a focus on calculation of biological equivalence (BEQ) concentrations. ISO 23196 specifies the derivation of biological equivalence (BEQ) concentrations for results of in vitro bioassays which are based on measuring effects on a biological process such as enzyme induction or cellular growth. The concept described in ISO 23196 is used for any biological assay after the proof of its applicability. To derive BEQ concentrations, the effect on a biological process caused by a sample – i.e., the activity of the sample – is expressed in terms of a concentration of a reference compound which results in an equivalent effect on the process. Who is ISO 23196 - Biological equivalence (BEQ) concentrations for ? ISO 23196 on biological equivalence (BEQ) concentrations is useful for: Water testing laboratories Municipal bodies Biology researchers Water treatment plants Why should you use ISO 23196 - Biological equivalence (BEQ) concentrations ?

Water quality. Calculation of biological equivalence (BEQ) concentrations

KS I ISO 18749-2022 水质——活性污泥对物质的吸附——使用特定分析方法的批量试验

Water quality ― Adsorption of substances on activated sludge ―Batch test using specific analytical methods

KS I ISO 8192-2022 水质.碳质和铵氧化用活性污泥抑制耗氧量的试验

Water quality — Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation

KS I ISO 14669-2022 水质.海洋桡足类（桡足类 甲壳类）急性致死毒性的测定

Water quality — Determination of acute lethal toxicity to marine copepods(Copepoda, Crustacea)

KS I ISO 14593-2022 水质水介质中有机化合物最终需氧生物降解性的评估密封容器中无机碳的分析方法（CO2顶空试验）

Water quality ― Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium — Method by analysis of inorganic carbon in sealed vessels(CO2 headspace test)

KS I ISO 15522:2022 水质.水成分对活性污泥微生物生长抑制作用的测定

Water quality — Determination of the inhibitory effect of water constituents on the growth of activated sludge microorganisms

KS I ISO 15522-2022 水质.水成分对活性污泥微生物生长抑制作用的测定

Water quality — Determination of the inhibitory effect of water constituents on the growth of activated sludge microorganisms

KS I ISO 8192:2022 水质.碳质和铵氧化用活性污泥抑制耗氧量的试验

Water quality — Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation

KS I ISO 18749:2022 水质——活性污泥对物质的吸附——使用特定分析方法的批量试验

Water quality ― Adsorption of substances on activated sludge ―Batch test using specific analytical methods

KS I ISO 14669:2022 水质.海洋桡足类（桡足类 甲壳类）急性致死毒性的测定

Water quality — Determination of acute lethal toxicity to marine copepods(Copepoda, Crustacea)

KS I ISO 14593:2022 水质水介质中有机化合物最终需氧生物降解性的评估密封容器中无机碳的分析方法（CO2顶空试验）

Water quality ― Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium — Method by analysis of inorganic carbon in sealed vessels(CO2 headspace test)

ISO 23196:2022 水质.生物当量（BEQ）浓度的计算

Water quality — Calculation of biological equivalence (BEQ) concentrations

BS EN ISO 16266-2:2021 水质 铜绿假单胞菌的检测和计数 最大可能数法

1   Scope This document specifies a method for the enumeration of Pseudomonas aeruginosa in water. The method is based on the growth of target organisms in a liquid medium and calculation of the most probable number (MPN) of organisms by reference to MPN tables. This document is applicable to a range of types of water. For example, hospital waters, drinking water and non-carbonated bottled waters intended for human consumption, groundwater, swimming pool and spa pool waters including those containing high background counts of heterotrophic bacteria. This document does not apply to carbonated bottled waters, flavoured bottle waters, cooling tower waters or marine waters, for which the method has not been validated. These waters are, therefore, outside the scope of this document. Laboratories can employ the method presented in this document for these matrices by undertaking appropriate validation of performance of this method prior to use. The test is based on a bacterial enzyme detection technology that signals the presence of P. aeruginosa through the hydrolysis of a 7-amino-4-methylcoumarin aminopeptidase substrate present in a special reagent. P. aeruginosa cells rapidly grow and reproduce using the rich supply of amino acids, vitamins and other nutrients present in the reagent. Actively growing strains of P. aeruginosa have an enzyme that cleaves the 7-amido-coumarin aminopeptidase substrate releasing a product which fluoresces under ultraviolet (UV) light. The test described in this document provides a confirmed result within 24 h with no requirement for further confirmation of positive wells.

Water quality. Detection and enumeration of Pseudomonas aeruginosa - Most probable number method

ASTM E2591-22 用两栖动物进行全沉积物毒性试验的标准指南

1.1 This standard covers procedures for obtaining laboratory data concerning the toxicity of test material (for example, sediment or hydric soil (that is, a soil that is saturated, flooded, or ponded long enough during the growing season to develop anaerobic (oxygen-lacking) conditions that favor the growth and regeneration of hydrophytic vegetation)) to amphibians. This test procedure uses larvae of the northern leopard frog (Lithobates pipiens). Other anuran species (for example, the green frog (Lithobates clamitans), the wood frog (Lithobates sylvatica), the American toad (Bufo americanus)) may be used if sufficient data on handling, feeding, and sensitivity are available. Test material may be sediments or hydric soil collected from the field or spiked with compounds in the laboratory. 1.2 The test procedure describes a 10-d whole sediment toxicity test with an assessment of mortality and selected sublethal endpoints (that is, body width, body length). The toxicity tests are conducted in 300 to 500-mL chambers containing 100 mL of sediment and 175 mL of overlying water. Overlying water is renewed daily and larval amphibians are fed during the toxicity test once they reach Gosner stage 25 (operculum closure over gills). The test procedure is designed to assess freshwater sediments, however, R. pipiens can tolerate mildly saline water (not exceeding about 2500 mg Cl/L, equivalent to a salinity of about 4.1 when Na+ is the cation) in 10-d tests, although such tests should always include a concurrent freshwater control. Alternative test durations and sublethal endpoints may be considered based on site-specific needs. Statistical evaluations are conducted to determine whether test materials are significantly more toxic than the laboratory control sediment or a field-collected reference sample(s). 1.3 Where appropriate, this standard has been designed to be consistent with previously developed methods for assessing sediment toxicity to invertebrates (for example, Hyalella azteca and Chironomus dilutus toxicity tests) described in the United States Environmental Protection Agency (USEPA, (1))2 freshwater sediment testing guidance, Test Methods E1367 and E1706, and Guides E1391, E1525, E1611, and E1688. Tests extending to 10 d or beyond, and including sublethal measurements such as growth, are considered more effective in identifying chronic toxicity and thus delineating areas of moderate contamination (1-3). 1.4 Many historical amphibian studies, both water and sediment exposure, have used tests of shorter duration (5 days or less) (for example, 4-7) and, although both survival and sublethal endpoints were often assessed, there is substantive evidence that tests of longer duration are likely to be more sensitive to some contaminants (8-10). Research performed to develop and validate this test protocol included long-term (through metamorphosis) investigations and other researchers have also conducted long-duration tests with anurans (7-20). Interestingly, some studies with anurans have shown significantly reduced growth (for example, whole body mass, snoutvent length) can be detected earlier in a longer-term test (for example, at 14-20 d), but cannot be statistically distinguished in older organisms later in the test (11, 14). In the development of these procedures, an attempt was made to balance the needs of a practical assessment with the importance of assessing longer-term effects so that the results will demonstrate the needed accuracy and precision. The most recent sediment toxicity testing protocols for invertebrates have encompassed longer duration studies which allow the measurement of reproductive endpoints (1, 21). Such tests, because of increased sensitivity of the sublethal endpoints, may also be helpful in evaluating toxicity. Full life-cycle studies with anurans (including reproduction) are usually not feasible from either a technical or monetary standpoint. However, if site-specific information indicates that the contaminants present are likely to affect other endpoints (including teratogenicity), then the duration of the toxicity test may be increased through metamorphosis or additional sublethal endpoints may be measured 1 This guide is under the jurisdiction of ASTM Committee E50 on Environmental Assessment, Risk Management and Corrective Action and is the direct responsibility of Subcommittee E50.47 on Biological Effects and Environmental Fate. Current edition approved Jan. 1, 2022. Published April 2022. Originally approved in 2007. Last previous edition approved in 2013 as E2591–07(2013). DOI: 10.1520/E2591-22. 2 The boldface numbers in parentheses refer to the list of references at the end of this standard. Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. 1 (for example, impaired behavior, deformities, time-tometamorphosis). The possible inclusion of these endpoints and extension of test length should be considered during development of the project or study plan (see 8.1.1). 1.5 The methodology presented in this standard was developed under a Department of Defense (DoD) research program and presented in a guidance manual for risk assessment staff and state/federal regulators involved in the review and approval of risk assessment work plans and reports (22). To develop this method, a number of tests with spiked sediment tests were conducted (22, 23). Since development of the methodology it has been used operationally to evaluate fieldcollected sediments from several state and federal environmental sites (24, 25). For most of these studies the preferred test organisms, Lithobates pipiens, was used. At a leadcontaminated state-led site, operated by the Massachusetts Highway Department, Xenopus laevis(African clawed frog) was used in the sediment test system because of availability problems with Lithobates pipiens (26), The test method was also used to evaluate sediment toxicity at a cadmiumcontaminated USEPA Region 4-led site in Tennessee (27). The methodology was used to help characterize potential effects of contaminants on amphibians and to help develop preliminary remedial goals, if warranted. All tests evaluated survival and growth effects after 10 d of exposure in accordance with the methods presented in this standard. 1.6 The use of larval amphibians to assess environmental toxicity is not novel. Researchers have used tadpoles to examine toxicity of metals and organic compounds. Most of these studies have been through water exposure, usually in a manner similar to fish or invertebrate exposure as described in Guide E729 (28-40). Fewer studies have focused on exposure of anuran larvae to sediments, and the methods employed vary widely, from in situ enclosures (15, 41) to laboratory tests using variable exposure conditions and organism ages (4, 8, 14, 39, 42-44). No studies were identified that used the same test conditions as described in this standard. However, several laboratory-based evaluations of sediment effects on amphibians are described in the following subsections. 1.6.1 Sediment toxicity tests conducted in the laboratory with amphibians were performed over a range of test durations from 4 d (4, 39, 42, Guide E1439-98 Appendix X2) to 12 d (44) and through metamorphosis (8, 14, 43). Sediment toxicity tests with anurans native to North America were started with larval tadpoles between Gosner stages 23 and 25 (8, 43, 44). Test temperatures were between 21 °C and 23 °C and feeding began after tadpoles reached Gosner stage 25. Food sources were TetraMin3 (8), boiled romaine lettuce (43), boiled romaine lettuce and flaked fish food (14), or boiled romaine lettuce and dissipated rabbit food pellets (44). Tests were conducted in static renewal mode with water replacements conducted at varying rates (daily (42, 44), weekly (8), every 3 to 5 d (43)). Test design (number of replicates, test vessel size, number of organisms per replicate) varied depending on the objective of the study with several tests conducted in aquaria (14, 43), large bins (8), or swimming pools (44). Endpoints evaluated at test termination included survival (4, 8, 14, 42-44), growth (8, 14, 42-44), bioaccumulation of metals (8), developmental rates (8, 14, 43), deformities (14, 42, 43), swimming speed (44) and foraging activity levels (43). 1.6.2 To assess the effect of direct contact with the sediments containing PCBs, Savage et al. (43) exposed larval tadpoles (Gosner stage 23 to 25; wood frogs (R. sylvatica)) to field-collected sediments under conditions that allowed both direct contact with the sediment and separation from the sediment with a 500 µm mesh barrier. The study found that lethal and sublethal effects on tadpoles observed through metamorphosis were more pronounced when direct contact with the sediment was allowed. Fuentes et al. (39) evaluated the acute toxicity of two Roundup4 (a widely used herbicide with the active ingredient glyphosate) formulations to six anuran species, including Lithobates pipiens, under both wateronly and water-+sediment conditions. The study found that toxicity of the glyphosate-based herbicides was reduced in the presence of sediment, likely due to sorption to sediment particles and associated organic matter. The test conditions described in this standard allow tadpoles to maintain direct contact with the sediment. 1.6.3 Sediment toxicity testing with Xenopus laevis has focused on evaluating the developmental effects of sediment extracts, as opposed to whole sediments, on frog embryos. Methods have been developed which expose blastula stage embryos to sediment by enclosing the embryos in a Teflon mesh insert that rests over the top of the sediment in the sediment–water interface region ((42), Guide E1439-98 Appendix X2). These studies are conducted evaluate survival, growth, and physical malformations of the embryos after a 4-d exposure period. The test conditions described in this standard allow more direct contact with the sediment, using older test organisms, and a longer exposure duration. 1.7 Amphibian species may be key receptors of potential chemicals of concern at contaminated sites. Although historically not often included in risk assessments, the importance of amphibians as both sensitive and keystone species is increasingly recognized, particularly considering the decline in amphibian worldwide populations, which may be driven by multiple localized stress agents rather than a single, dominating cause (45). The lack of amphibian representation as surrogate species is likely due to multiple factors including scant knowledge of local amphibian populations and life histories, the paucity of applicable toxicity data, and inconsistency in standardized assessment protocols. A review of ecological risk assessment methods for amphibians and gaps in existing amphibian toxicity data and methods is provided by Johnson et al. (46). The importance of amphibians in the ecological risk assessment process is recognized by Environment and Climate Change Canada in the Ecological Risk Assessment Guidance under the Federal Contaminated Sites Action Plan (47). Sediment toxicity tests are an effective means for evaluating the impact of sediment contamination on amphibians in a multiple lines of evidence paradigm. The evaluation is most powerful 3 TetraMin is a trademark of TETRA GMBH. 4 Roundup is a registered trademark of Monsanto Company. E2591 − 22 2 when toxicity testing sampling stations are co-located with sediment analytical chemistry samples and ecological surveys, allowing for a detailed evaluation of the co-occurring data in the ecological risk assessment. The spatial and temporal co-location of toxicity testing and analytical samples is particularly important for establishing contaminant-specific effects and assessing contaminant bioavailability. 1.8 In order for a sediment toxicity test to be sensitive it must be of sufficient duration to measure potential toxicity and it must be conducted during the appropriate developmental stage of the test organism’s life cycle. Using recently hatched tadpoles and conducting the sediment exposure test for 10 d to allow the evaluation of growth endpoints meets both of these sensitivity requirements. 1.9 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.10 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.11 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Standard Guide for Conducting Whole Sediment Toxicity Tests with Amphibians

DIN EN ISO 10872:2021 水和土壤质量 沉积物和土壤样品对秀丽隐杆线虫（线虫）生长、肥力和繁殖的毒性作用的测定（ISO 10872:2020）；德文版 EN ISO 10872:2021

This document specifies a method for determining the toxicity of environmental samples on growth, fertility and reproduction of Caenorhabditis elegans. The method applies to contaminated whole freshwater sediment (maximum salinity 5 g/l), soil and waste, as well as to pore water,

Water and soil quality - Determination of the toxic effect of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans (Nematoda) (ISO 10872:2020); German version EN ISO 10872:2021

KS I ISO TR 11044-2021 水质 批量藻类生长抑制试验的科学技术问题

Water quality－Scientific and technical aspects of batch algae growth inhibition tests

KS I ISO 9408-2021 水质 通过密闭呼吸计测定需氧量来评价水介质中有机化合物的最终需氧生物降解性

Water quality－Evaluation of ultimate aerobic biodegradability of organic compounds in aqueous medium by determination of oxygen demand in a closed respirometer