13.060.70 水的生物学特性的检验 标准查询与下载

KS I ISO 20079-2021 水质 水成分和废水对浮萍的毒性作用测定 浮萍生长抑制试验

Water quality－Determination of the toxic effect of water constituents and waste water on duckweed (Lemna minor)－Duckweed growth inhibition test

KS I ISO 10706-2021 水质 物质对大型溞（枝角类、甲壳类）长期毒性的测定

Water quality－Determination of long term toxicity of substancesto Daphnia magna Straus(Cladocera, Crustacea)

KS I ISO 16221-2021 水质 海洋环境中生物降解性测定导则

Water quality－Guidance for determination of biodegradability in the marine environment

KS I ISO 20666-2021 水质 萼花臂尾轮虫48h慢性毒性测定

Water quality－Determination of the chronic toxicity to Brachionus calyciflorus in 48h

KS I ISO 16240-2021 水质 水和废水遗传毒性的测定 沙门氏菌/微粒体试验（Ames试验）

Water quality－Determination of the genotoxicity of water andwaste water－Salmonella/microsome test(Ames test)

KS I ISO 12890-2021 水质 淡水鱼胚胎和幼体毒性测定 半静态法

Water quality－Determination of toxicity to embryos and larvae of freshwater fish－Semi-static method

KS I ISO 13829-2021 水质-使用umu试验测定水和废水的遗传毒性

Water quality-Determination of the genotoxicity of water and waste water using the umu-test

KS I ISO 20665-2021 水质 对毛溞慢性毒性的测定

Water quality－Determination of chronic toxicity to Ceriodaphnia dubia

EN ISO 16266-2:2021 水质.去硝假单胞菌的检测及计数.第2部分:最可能数法

This document specifies a method for the enumeration of Pseudomonas aeruginosa in water. The method is based on the growth of target organisms in a liquid medium and calculation of the most probable number (MPN) of organisms by reference to MPN tables. This document is applicable to a range of types of water. For example, hospital waters, drinking water and non‑carbonated bottled waters intended for human consumption, groundwater, swimming pool and spa pool waters including those containing high background counts of heterotrophic bacteria. This document does not apply to carbonated bottled waters, flavoured bottle waters, cooling tower waters or marine waters, for which the method has not been validated. These waters are, therefore, outside the scope of this document. Laboratories can employ the method presented in this document for these matrices by undertaking appropriate validation of performance of this method prior to use. The test is based on a bacterial enzyme detection technology that signals the presence of P. aeruginosa through the hydrolysis of a 7‑amino‑4‑methylcoumarin aminopeptidase substrate present in a special reagent. P. aeruginosa cells rapidly grow and reproduce using the rich supply of amino acids, vitamins and other nutrients present in the reagent. Actively growing strains of P. aeruginosa have an enzyme that cleaves the 7‑amido‑coumarin aminopeptidase substrate releasing a product which fluoresces under ultraviolet (UV) light. The test described in this document provides a confirmed result within 24 h with no requirement for further confirmation of positive wells.

Water quality - Detection and enumeration of Pseudomonas aeruginosa - Part 2: Most probable number method (ISO 16266-2:2018)

ASTM E1611-21 用多毛环节动物进行沉积物毒性试验的标准指南

1.1 This guide covers procedures for obtaining laboratory data concerning the adverse effects of potentially contaminated sediment, or of a test material added experimentally to contaminated or uncontaminated sediment, on marine or estuarine infaunal polychaetes during 10-day or 20 to 28-day exposures. These procedures are useful for testing the effects of various geochemical characteristics of sediments on marine and estuarine polychaetes and could be used to assess sediment toxicity to other infaunal taxa, although modifications of the procedures appropriate to the test species might be necessary. Procedures for the 10-day static test are described for Neanthes arenaceodentata and Alitta virens 2 (formerly Nereis virens and Neanthes virens) and for the 20 to 28-day static-renewal sediment toxicity for N. arenaceodentata. 1.2 Modifications of these procedures might be appropriate for other sediment toxicity test procedures, such as flowthrough or partial life-cycle tests. The methods outlined in this guide should also be useful for conducting sediment toxicity tests with other aquatic taxa, although modifications might be necessary. Other test organisms might include other species of polychaetes, crustaceans, and bivalves. 1.3 Other modifications of these procedures might be appropriate for special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, the results of tests conducted using unusual procedures are not likely to be comparable to those of many other tests. Comparisons of the results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting sediment tests with infaunal organisms. 1.4 These procedures are applicable to sediments contaminated with most chemicals, either individually or in formulations, commercial products, and known or unknown mixtures. These procedures can be used with appropriate modifications to conduct sediment toxicity tests on factors such as temperature, salinity, dissolved oxygen (DO), and natural sediment characteristics (for example, particle size distribution, organic carbon content, and total solids). These procedures can also be used to conduct bioconcentration tests and in situ tests, and to assess the toxicity of potentially contaminated field sediments, or of materials such as sewage sludge, oils, particulate matter, and solutions of toxicants added to sediments. A median lethal concentration (LC50) or median sublethal effect concentration (EC50) of toxicants or of highly contaminated sediment mixed into uncontaminated sediment can be determined. Materials adhering to sediment particles or dissolved in interstitial water can be tested. 1.5 The results of 10-day toxicity tests with contaminated sediments can be reported as a LC50 if a series of concentrations is tested or as a percent mortality relative to a control or reference sediment. The results of 20 to 28-day toxicity tests with contaminated sediments can be reported as a LC50 if a series of concentrations is tested or as a percent mortality or growth relative to a control or reference sediment. 1.6 This guide is arranged as follows: Section Referenced Documents 2 Terminology 3 Summary of Guide 4 Significance and Use 5 Interferences 6 Apparatus 7 Facilities 7.1 Construction Materials 7.2 Test Chambers 7.3 Cleaning 7.4 Acceptability 7.5 Hazards 8 Test Water 9 General Requirements 9.1 Source 9.2 Preparation 9.3 Characterization 9.4 Test and Control Sediments 10 General 10.1 Characterization 10.2 Control Sediment 10.3 Field-Collected Test Sediment 10.4 Reference Sediment 10.5 Laboratory-Spiked Test Sediment 10.6 1 This guide is under the jurisdiction of ASTM Committee E50 on Environmental Assessment, Risk Management and Corrective Action and is the direct responsibility of Subcommittee E50.47 on Biological Effects and Environmental Fate. Current edition approved Nov. 1, 2021. Published December 2021. Originally approved in 1994. Last previous edition approved in 2013 as E1611 00(2013). DOI: 10.1520/E1611-21. 2 World Register of Marine Species (WoRMS) at https://www.marinespecies.org/aphia.php?p=taxdetails&id=234851 *A Summary of Changes section appears at the end of this standard Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. 1 Section Test Concentration(s) 10.7 Addition of Toxicant to Sediment 10.8 Test Organisms 11 Species 11.1 Age 11.2 Feeding 11.3 Source 11.4 Collection and Handling 11.5 Quality 11.6 Experimental Design 12 Controls 12.2 Field Survey Design 12.3 Laboratory Experiments 12.4 Procedure 13 Dissolved Oxygen 13.1 Temperature 13.2 Salinity 13.3 Light 13.4 Feeding 13.5 Beginning of Test 13.6 Duration of Test 13.7 Biological Data 13.8 Other Measurements 13.9 Analytical Methodology 14 Acceptability of Test 15 Interpretation of Results 16 Report 17 Keywords 18 Annexes Neanthes arenaceodentata Annex A1 Alitta virens Annex A2 1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. Specific hazards statements are given in Section 8. 1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Standard Guide for Conducting Sediment Toxicity Tests with Polychaetous Annelids

ASTM D3978-21a 用伪基氏藻进行藻类生长潜力试验的标准实施规程

1.1 This practice measures, by Pseudokirchnereilla subcapitata growth response, the biological availability of nutrients, as contrasted with chemical analysis of the components of the sample. This practice is useful for assessing the impact of nutrients, and changes in their loading, upon freshwater algal productivity. Other laboratory or indigenous algae can be used with this practice. However, Pseudokirchnereilla subcapitata must be cultured as a reference alga along with the alternative algal species. 1.2 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For a specific precautionary statement, see Section 16. 1.3 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Standard Practice for Algal Growth Potential Testing with Pseudokirchneriella subcapitata

LST EN ISO 10872:2021 水和土壤质量 沉积物和土壤样本对秀丽隐杆线虫（线虫）生长、肥力和繁殖的毒性作用的测定（ISO 10872:2020）

Water and soil quality - Determination of the toxic effect of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans (Nematoda) (ISO 10872:2020)

UNI EN ISO 10872:2021 水和土壤质量 沉积物和土壤样品对秀丽隐杆线虫（线虫）生长、生育力和繁殖的毒性作用的测定

Water and soil quality - Determination of the toxic effect of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans (Nematoda)

T/SDAQI 007-2021 生产用水中铜绿假单胞菌的快速定性检测 实时荧光PCR方法

样品经抽滤设备过滤后，将过滤后的膜放入选择培养基中培养。收集培养后的菌液进行DNA提取。以DNA为模板，采用细菌16S rDNA基因序列作为内参照，以铜绿假单胞菌中外毒素A（eta）基因中相对保守且高度特异的引物和探针进行目标物的实时荧光PCR扩增，根据Ct值，判断样品中是否含有铜绿假单胞菌。其中，对内参照反应的检测，可以监控反应是否正常进行，防止假阴性结果。

Rapid qualitative detection of Pseudomonas aeruginosa in production water Real-time PCR method

BS EN ISO 10872:2021 水和土壤质量 测定沉积物和土壤样品对秀丽隐杆线虫（线虫）生长、繁殖和繁殖的毒性作用

1   Scope This document specifies a method for determining the toxicity of environmental samples on growth, fertility and reproduction of Caenorhabditis elegans . The method applies to contaminated whole freshwater sediment (maximum salinity 5 g/l), soil and waste, as well as to pore water, elutriates and aqueous extracts that were obtained from contaminated sediment, soil and waste.

Water and soil quality. Determination of the toxic effect of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans (Nematoda)

DS/EN ISO 10872:2021 水和土壤质量 沉积物和土壤样品对秀丽隐杆线虫（线虫）生长、肥力和繁殖的毒性作用的测定（ISO 10872:2020）

Water and soil quality – Determination of the toxic effect of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans (Nematoda) (ISO 10872:2020)

OENORM EN ISO 16266-2:2021 水质-铜绿假单胞菌的检测和计数-第2部分：最可能数法（ISO 16266-2:2018）

Water quality - Detection and enumeration of Pseudomonas aeruginosa - Part 2: Most probable number method (ISO 16266-2:2018)

EN ISO 10872:2021 水质.沉淀物和土壤样本上的克氏病(线虫纲)的生长、生育和繁殖毒性的测定

Water and soil quality - Determination of the toxic effect of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans (Nematoda) (ISO 10872:2020)

SN EN ISO 10872:2021 水和土壤质量 沉积物和土壤样本对秀丽隐杆线虫（线虫）生长、肥力和繁殖的毒性作用的测定（ISO 10872:2020）

Water and soil quality - Determination of the toxic effect of sediment and soil samples on growth, fertility and reproduction of Caenorhabditis elegans (Nematoda) (ISO 10872:2020)

DS/ISO 16266-2:2021 水质《铜绿假单胞菌检测与计数》第2部分：最可能数法

Water quality – Detection and enumeration of Pseudomonas aeruginosa – Part 2: Most probable number method