【课件】荧光光谱分析方法及原理

　　荧光光谱

　　参考书

　　赵南明，周海梦. 生物物理学，高等教育出版社，2000

　　Emission Spectroscopy, Chapter 11, in K.E. van Holde, W.C. Johnson and P.S. Ho eds. Principles of Physical Biochemistry, Prentice-Hall, 1998

　　某些物质被一定波长的光照射时，会在一定时间内发射出波长比入射光长的光，如果这个时间比较短，这种光就称为荧光。荧光由一种能发荧光的矿物 ¾¾ 萤石(fluospar)而得名。

　　我们这里要介绍的荧光，是指物质在吸收紫外光和可见光后发出的波长较长的紫外荧光或可见荧光。

　　除了紫外光和可见光可能激发荧光外，其它的光如红外光、X射线也可能激发出荧光，因此除紫外荧光或可见荧光外，还有红外荧光、X射线荧光等。

　　·In discussing absorption we have been concerned entirely with the excitation of a molecule from its ground state to a higher energy level and have given no consideration to the subsequent fate of the excited molecule.

　　· In many cases the sequel is not very interesting; the energy is transferred as heat to the surroundings.

　　· In some cases light is emitted by the sample either as fluorescence or phosphorescence.

　　· We will discuss the general characteristics of emission and ignore the exceptions.