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042.Abbkine精品推荐------DyLight 488绿色荧光二抗！
上一篇 / 下一篇 2015-11-19 09:09:55/ 个人分类：新技术港
DyLigh™系列荧光标记是一种近年来被广泛应用的新型荧光染料，由于具有很好的光谱宽度、更强的荧光强度，更高的光学耐受性（抗淬灭性）、对pH不敏感、分子较小而渗透性更好的优势，越来越受到科研客户的喜爱。DyLight™种类齐全，如Dylight 405是蓝色荧光，替代Alexa 405；Dylight 488是绿色荧光，可完全替代FITC/Cy2/Alexa 488；完全替代Cy3/Rhodamin/Alexa 546/Alexa 555的Dylight 549；替代Texas Red/Alexa 594的Dylight 594；Dylight 633和Dylight 649（可替代Cy5/Alexa 647）则是更强的红色荧光；另外，Dylight还有适配Odyssey系统的近红外和远红外二抗Dylight 680和DyLight 800。
图1：HEp-2细胞使用ANA一抗标记，并比较使用不同发色团的二抗标记。荧光显微镜下，上排图片的细胞用ProLong Gold (Invitrogen)封片，下排细胞用含N-没食子酸丙酯（抗淬灭剂）的PBS-90%甘油封片。Nikon LABOPHOTNikon 荧光显微镜连接D-70 DSLR 摄像机，100×曝光1秒拍摄的图片。
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| 图2：使用 FACSCalibur流式细胞仪(Becton Dickinson)，分析抗-CD3和其他荧光素标记的人外周血淋巴细胞图谱。DyLight 488 (绿色)、FITC (黄色)、Cy2 (蓝色)标记的二抗都来自Jackson公司。另外Alexa Fluor 488购自Invitrogen。箭头所指处显示Alexa Fluor 488在这个实验中有轻微的背景。 | 图3：以时间为横坐标，初始荧光强度的百分比为纵坐标绘制的图谱，HEp-2细胞以ANA（Bio-Rad）和各种山羊抗人IgG（H+L）的二抗标记， 细胞用不含抗淬灭剂的PBS-90%甘油封片。显示DyLight 488和Alexa 488均有较好的光学稳定性，FITC最容易淬灭，Cy2次之。 | ||
DyLight 488标记的抗体比Cy2 和FITC标记的更亮，与Alexa Fluor 488 标记的亮度相当，但背景更低；从光稳定性上来说，DyLight 488与Alexa 488均表现优异，均强于FITC和Cy2。另外，DyLight荧光标记的二抗比传统的FITC/Cy2以及Alexa 488具有更高的性价比。
我们向您推荐美国Abbkine原装进口的液体形式的小包装常用DyLight系列二抗产品，以及Jackson各种规格的全系列DyLight二抗产品。Abbkine是美国专业生产免疫试剂并提供细胞学研究解决方案的公司，其产品主要销往到全球的的大学、科研院所等研究机构。其中Abbkine为您提供最常用的DyLight 488荧光标记二抗产品：
| 品名 | 品牌 | 货号 | 规格 |
| DyLight 488 AffiniPure Goat Anti-Mouse IgG(H+L) | Abbkine | A23210 | 100μl/500μl |
| DyLight 488 AffiniPure Goat Anti-Rabbit IgG(H+L) | Abbkine | A23220 | 100μl/500μl |
| DyLight 488 AffiniPure Rabbit Anti-Goat IgG(H+L) | Abbkine | A23230 | 100μl/500μl |
| DyLight 488 AffiniPure Goat Anti-Rat IgG(H+L) | Abbkine | A23240 | 100μl/500μl |
Jackson是全球专业从事生产和销售亲和纯化的二抗和纯化免疫球蛋白的公司，其抗体产品线完全，有全球最大的二抗产品库，能提供多种应用类型和需要的二抗产品，其二抗产品本身也具有极高的产品质量和稳定性，其产品主要销往到世界各地的大学科研所和医院里的动物研究以及生物医学研究机构的科学家。Jackson能为您提供最全的DyLight 488荧光标记二抗：
1、Jackson DyLight 488抗小鼠IgG二抗产品如下：
| 品名 | 品牌 | 货号 | 规格 |
| DyLight 488 AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot) | Jackson | 715-485-150 | 0.5mg |
| DyLight 488 AffiniPure Donkey Anti-Mouse IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Rat, Shp Sr Prot) | Jackson | 715-485-151 | 0.5mg |
| DyLight 488 AffiniPure Donkey Anti-Mouse IgM, µ chain specific | Jackson | 715-485-020 | 2.0mg |
| DyLight 488 AffiniPure Donkey Anti-Mouse IgM, µ chain specific (min X Hu, Bov, Hrs, Rat Sr Prot) | Jackson | 715-485-140 | 0.5mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgG (H+L) | Jackson | 115-485-003 | 2.0mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) | Jackson | 115-485-062 | 1.5mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs, Rb, Sw Sr Prot) | Jackson | 115-485-146 | 1.5mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgG (H+L) (min X Rat, Hu, Bov, Hrs, Rb, Sr Prot) | Jackson | 115-485-166 | 0.5mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgG, Light Chain Specific for Western blotting (min X Bov, Gt, Hrs, Hu, Rb, Rat, Shp Ig) | Jackson | 115-485-174 | 0.5mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgG, Fcγ fragment specific | Jackson | 115-485-008 | 2.0mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgG, Fcγ fragment specific (min X Hu, Bov, Hrs Sr Prot) | Jackson | 115-485-071 | 1.5mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 1 specific (min X Hu, Bov, Rb Sr Prot) | Jackson | 115-485-205 | 0.5mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2a specific (min X Hu, Bov, Rb Sr Prot) | Jackson | 115-485-206 | 0.5mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2b specific (min X Hu, Bov, Rb Sr Prot) | Jackson | 115-485-207 | 0.5mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 2c specific (min X Hu, Bov, Rb Sr Prot) | Jackson | 115-485-208 | 0.5mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgG, Fcγ Subclass 3 specific (min X Hu, Bov, Rb Sr Prot) | Jackson | 115-485-209 | 0.5mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgG (subclasses 1+2a+2b+3), Fcγ fragment specific (min X Hu,Bov, Rb Sr Prot) | Jackson | 115-485-164 | 1.0mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgG, F(ab')2 fragment specific | Jackson | 115-485-006 | 2.0mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgG, F(ab')2 fragment specific (min X Hu, Bov, Hrs Sr Prot) | Jackson | 115-485-072 | 1.5mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgG + IgM (H+L) | Jackson | 115-485-044 | 2.0mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgG + IgM(H+L) (min X Hu, Bov,Hrs Sr Prot) | Jackson | 115-485-068 | 1.5mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgM, µ chain specific | Jackson | 115-485-020 | 2.0mg |
| DyLight 488 AffiniPure Goat Anti-Mouse IgM, µ chain specific (min X Hu, Bov, Hrs Sr Prot) | Jackson | 115-485-075 | 1.0mg |
| DyLight 488 AffiniPure Rabbit Anti-Mouse IgG (H+L) | Jackson | 315-485-003 | 1.5mg |
| DyLight 488 AffiniPure Rabbit Anti-Mouse IgG (H+L) (min X Hu Sr Prot) | Jackson | 315-485-045 | 1.0mg |
| DyLight 488 AffiniPure Rabbit Anti-Mouse IgG, Fcγ fragment specific | Jackson | 315-485-008 | 1.5mg |
| DyLight 488 AffiniPure Rabbit Anti-Mouse IgG, Fcγ fragment specific (min X Hu Sr Prot) | Jackson | 315-485-046 | 1.0mg |
| DyLight 488 AffiniPure Rabbit Anti-Mouse IgG, F(ab')2 fragment specific | Jackson | 315-485-006 | 1.5mg |
| DyLight 488 AffiniPure Rabbit Anti-Mouse IgG, F(ab')2 fragment specific (min X Hu Sr Prot) | Jackson | 315-485-047 | 1.0mg |
| DyLight 488 AffiniPure Rabbit Anti-Mouse IgG + IgM (H+L) | Jackson | 315-485-044 | 1.5mg |
| DyLight 488 AffiniPure Rabbit Anti-Mouse IgG + IgM (H+L) (min X Hu Sr Prot) | Jackson | 315-485-048 | 1.0mg |
| DyLight 488 AffiniPure Rabbit Anti-Mouse IgM, µ chain specific | Jackson | 315-485-020 | 1.5mg |
| DyLight 488 AffiniPure Rabbit Anti-Mouse IgM, µ chain specific (min X Hu Sr Prot) | Jackson | 315-485-049 | 1.0mg |
| DyLight 488 AffiniPure Rat Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs, Rat, Rb Sr Prot) | Jackson | 415-485-166 | 1.0mg |
| DyLight 488 AffiniPure Sheep Anti-Mouse IgG (H+L) | Jackson | 515-485-003 | 2.0mg |
| DyLight 488 AffiniPure Sheep Anti-Mouse IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) | Jackson | 515-485-062 | 1.5mg |
| DyLight 488 AffiniPure Sheep Anti-Mouse IgG, Fcγ fragment specific (min X Hu, Bov, Hrs Sr Prot) | Jackson | 515-485-071 | 1.5mg |
| DyLight 488 AffiniPure Sheep Anti-Mouse IgG, F(ab')2 fragment specific (min X Hu, Bov, Hrs Sr Prot) | Jackson | 515-485-072 | 1.5mg |
2、Jackson DyLight 488抗兔IgG二抗产品如下：
| 品名 | 品牌 | 货号 | 规格 |
| DyLight 488 AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rat, Shp Sr Prot) | Jackson | 711-485-152 | 0.5mg |
| DyLight 488 AffiniPure Goat Anti-Rabbit IgG (H+L) | Jackson | 111-485-003 | 2.0mg |
| DyLight 488 AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu Sr Prot) | Jackson | 111-485-045 | 1.5mg |
| DyLight 488 AffiniPure Goat Anti-Rabbit IgG (H+L) (min X Hu, Ms, Rat Sr Prot) | Jackson | 111-485-144 | 1.5mg |
| DyLight 488 AffiniPure IgG Fraction Monoclonal Mouse Anti-Rabbit IgG, Light Chain Specific for Western blotting (min X Bov, Gt, Ar Hms, Hrs, Hu, Ms, Rat, Shp Ig) | Jackson | 211-482-171 | 0.5mg |
| DyLight 488 AffiniPure Goat Anti-Rabbit IgG, Fc fragment specific | Jackson | 111-485-008 | 2.0mg |
| DyLight 488 AffiniPure Goat Anti-Rabbit IgG, Fc fragment specific (min X Hu Sr Prot) | Jackson | 111-485-046 | 1.5mg |
| DyLight 488 AffiniPure Goat Anti-Rabbit IgG, F(ab')2 fragment specific | Jackson | 111-485-006 | 2.0mg |
| DyLight 488 AffiniPure Goat Anti-Rabbit IgG, F(ab')2 fragment specific (min X Hu Sr Prot) | Jackson | 111-485-047 | 1.5mg |
| DyLight 488 AffiniPure Polyclonal Mouse Anti-Rabbit IgG (H+L) (min X Hu, Gt, Ms, Shp Sr Prot) | Jackson | 211-485-109 | 1.0mg |
3、Jackson DyLight 488抗山羊二抗产品如下：
| 品名 | 品牌 | 货号 | 规格 |
| DyLight 488 AffiniPure Bovine Anti-Goat IgG (H+L) (min X Bov, Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot) | Jackson | 805-485-180 | 0.5mg |
| DyLight 488 AffiniPure Donkey Anti-Goat IgG (H+L) | Jackson | 705-485-003 | 1.0mg |
| DyLight 488 AffiniPure Donkey Anti-Goat IgG (H+L) (min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot) | Jackson | 705-485-147 | 0.5mg |
| DyLight 488 AffiniPure Mouse Anti-Goat IgG (H+L) (min X Ms, Hu, Rb Sr Prot) | Jackson | 205-485-108 | 1.0mg |
| DyLight 488 AffiniPure Rabbit Anti-Goat IgG (H+L) | Jackson | 305-485-003 | 1.5mg |
| DyLight 488 AffiniPure Rabbit Anti-Goat IgG (H+L) (min X Hu Sr Prot) | Jackson | 305-485-045 | 1.0mg |
| DyLight 488 AffiniPure Rabbit Anti-Goat IgG, Fc fragment specific | Jackson | 305-485-008 | 1.5mg |
| DyLight 488 AffiniPure Rabbit Anti-Goat IgG, Fc fragment specific (min X Hu Sr Prot) | Jackson | 305-485-046 | 1.0mg |
| DyLight 488 AffiniPure Rabbit Anti-Goat IgG, F(ab')2 fragment specific | Jackson | 305-485-006 | 1.5mg |
| DyLight 488 AffiniPure Rabbit Anti-Goat IgG, F(ab')2 fragment specific (min X Hu Sr Prot) | Jackson | 305-485-047 | 1.0mg |
4、Jackson DyLight 488抗大鼠二抗产品如下：
| 品名 | 品牌 | 货号 | 规格 | |||||||||||||||||||||||||||||||||||||||||||||||||
| DyLight 488 AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Rb, Shp Sr Prot) | Jackson | 712-485-150 | 0.5mg | |||||||||||||||||||||||||||||||||||||||||||||||||
| DyLight 488 AffiniPure Donkey Anti-Rat IgG (H+L) (min X Bov, Ck, Gt, GP, Sy Hms, Hrs, Hu, Ms, Rb, Shp Sr Prot) | Jackson | 712-485-153 | 0.5mg | |||||||||||||||||||||||||||||||||||||||||||||||||
| DyLight 488 AffiniPure Goat Anti-Rat IgG (H+L) | Jackson | 112-485-003 | 2.0mg | |||||||||||||||||||||||||||||||||||||||||||||||||
| DyLight 488 AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu, Bov, Hrs Sr Prot) | Jackson | 112-485-062 | 1.5mg | |||||||||||||||||||||||||||||||||||||||||||||||||
| DyLight 488 AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu, Bov, Hrs, Rb Sr Prot) | Jackson | 112-485-143 | 1.5mg | |||||||||||||||||||||||||||||||||||||||||||||||||
| DyLight 488 AffiniPure Goat Anti-Rat IgG (H+L) (min X Ms, Hu, Bov, Hrs, Rb Sr Prot) | Jackson | 112-485-167 | 0.5mg | |||||||||||||||||||||||||||||||||||||||||||||||||
| DyLight 488 AffiniPure Goat Anti-Rat IgG, Light Chain Specific for Western blotting (min X Bov, Gt, Hrs, Hu, Ms, Rat, Shp Ig) | Jackson | 112-485-175 | 0.5mg | |||||||||||||||||||||||||||||||||||||||||||||||||
| DyLight 488 AffiniPure Goat Anti-Rat IgG, Fcγ fragment specific | Jackson | 112-485-008 | 2.0mg | |||||||||||||||||||||||||||||||||||||||||||||||||
| DyLight 488 AffiniPure Goat Anti-Rat IgG, Fcγ fragment specific (min X Hu, Bov, Hrs Sr Prot) | Jackson | 112-485-071 | 1.5mg | |||||||||||||||||||||||||||||||||||||||||||||||||
| DyLight 488 AffiniPure Goat Anti-Rat IgG, F(ab')2 fragment specific | Jackson | 112-485-006 | 2.0mg | |||||||||||||||||||||||||||||||||||||||||||||||||
| DyLight 488 AffiniPure Goat Anti-Rat IgG, F(ab')2 fragment specific (min X Hu, Bov, Hrs Sr Prot) | Jackson | 112-485-072 | 1.5mg | |||||||||||||||||||||||||||||||||||||||||||||||||
| DyLight 488 AffiniPure Goat Anti-Rat IgG + IgM (H+L) | Jackson | 112-485-044 | 2.0mg | |||||||||||||||||||||||||||||||||||||||||||||||||
| DyLight 488 AffiniPure Goat Anti-Rat IgG + IgM (H+L) (min X Hu, Bov, Hrs Sr Prot) | Jackson | 112-485-068 | 1.5mg | |||||||||||||||||||||||||||||||||||||||||||||||||
| DyLight 488 AffiniPure Goat Anti-Rat IgM, µ chain specific | Jackson | 112-485-020 | 2.0mg | |||||||||||||||||||||||||||||||||||||||||||||||||
| DyLight 488 AffiniPure Goat Anti-Rat IgM, µ chain specific (min X Hu, Bov, Hrs Sr Prot) | Jackson | 112-485-075 | 1.0mg | |||||||||||||||||||||||||||||||||||||||||||||||||
| DyLight 488 AffiniPure Mouse Anti-Rat IgG (H+L) (min X Ms Sr Prot) | Jackson | 212-485-082 | 1.5mg | |||||||||||||||||||||||||||||||||||||||||||||||||
| DyLight 488 AffiniPure Mouse Anti-Rat IgG (H+L) (min X Hu, Bov, Hrs, Ms, Gt, Rb Sr Prot) | Jackson | 212-485-168 | 1.0mg | |||||||||||||||||||||||||||||||||||||||||||||||||
| DyLight 488 AffiniPure
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