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Plant Sci:湖南农大丨氮素利用效率与镉耐受性的平衡(附NMT实验体系)
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下一篇 2019-07-18 10:22:08/ 个人分类:文献精选
期刊:plant science
主题:氮素利用效率与镉耐受性的平衡
标题:Balance between nitrogen use efficiency and cadmium tolerance in Brassica napus and Arabidopsis thaliana
影响因子:3.785
检测指标: H+、Cd2+、NO3-流速
检测部位:根部液泡
H+、Cd2+、NO3-流实验处理方法:
4周龄拟南芥,20 μM CdCl2处理3天
21日龄油菜,100 μM CdCl2处理7天
H+、Cd2+、NO3-流实验测试液成份:
文献无
推荐测试液成份:
拟南芥根部液泡:0.002mM CdCl2,0.1 mM KNO3 , 600 mM mannitol, 0.05mM MES, pH 7.2
油菜根部液泡:0.01mM CdCl2,0.1 mM KNO3 , 600 mM mannitol, 0.05mM MES, pH 7.2
作者:湖南农业大学张振华、廖琼
英文摘要
The transmembrane transport of NO3− andCd2+ into
plant cell vacuoles relies on the energy from their tonoplast
protonpumps, V-ATPase and V-PPase. If the activity of these pumps is
reduced, itresults in less NO3− and Cd2+ being transported into the
vacuoles, whichcontributes to better nitrogen use efficiency (NUE) and
lower Cd2+ tolerance inplants.
The physiological mechanisms that regulatethe balance
between NUE and Cd2+ tolerance remain unknown. In our study,
twoBrassica napus genotypes with differential NUEs, xiangyou 15 and 814,
andAtclca-2 mutant and AtCAX4 over-expression line (AtCAX4-OE) of
Arabidopsisthaliana, were used to investigate Cd2+ stress responses.
We found that the Brassica napus genotype,with higher
NUE, was more sensitive to Cd2+ stress. The AtCAX4-OE mutant,
withhigher Cd2+ vacuolar sequestration capacity (VSC), limited NO3−
sequestrationinto root vacuoles and promoted NUE. Atclca-2 mutants, with
decreased NO3− VSC,enhanced Cd2+ sequestration into root vacuoles and
conferred greater Cd2+tolerance than the WT. This may be due to the
competition between Cd2+ andNO3−in the vacuoles for the energy provided
by V-ATPase and V-PPase. Regulating thebalance between Cd2+ and NO3−
vacuolar accumulation by inhibiting the activityof CLCa transporter and
increasing the activity of CAX4 transporter willsimultaneously enhance
both the NUE and Cd2+ tolerance of Brassica napus,essential for
improving its Cd2+ phytoremediation potential.
中文摘要(谷歌机翻)
NO3-和Cd2+跨膜转运到植物细胞液泡中依赖于其液泡膜质子泵,V-ATP酶和V-PPase的能量。如果这些泵的活性降低,则导致较少的NO3-和Cd2+被输送到液泡中,这有助于提高植物的氮利用效率(NUE)和降低Cd2+耐受性。
调节NUE和Cd2+耐受性之间平衡的生理机制尚不清楚。在我们的研究中,使用具有差异NUE,香优15和814的两种甘蓝型油菜基因型和拟南芥的Atclca-2突变体和AtCAX4过表达系(AtCAX4-OE)来研究Cd2+应激反应。
我们发现甘蓝型油菜基因型具有较高的NUE,对Cd2+胁迫更敏感。具有较高Cd2+液泡隔离能力(VSC)的AtCAX4-OE突变体限制NO3-隔离到根空泡中并促进NUE。具有降低的NO3-VSC的Atclca-2突变体增强了Cd2+隔离到根空泡中并且赋予比WT更大的Cd2+耐受性。这可能是由于V-ATP酶和V-PPase提供的能量在液泡中Cd2+和NO3-之间的竞争。通过抑制CLCa转运蛋白的活性和增加CAX4转运蛋白的活性来调节Cd2+和NO3-液泡积累之间的平衡将同时增强甘蓝型油菜的NUE和Cd2+耐受性,这对于改善其Cd2+植物修复潜力是必需的。
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