中英文说明书丨艾美捷卡那霉素ELISA试剂盒方案

艾美捷卡那霉素ELISA试剂盒英文说明书：

Format

96-Well Plate

Species Reactivity

General

Sample Type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Detection Range

617.3-50,000pg/mL

Sensitivity

253.4pg/mL

Estimated Turnaround Time

7-11 business days

Target Name

KNM / Kanamycin

Target Synonyms

Kanamycin A

Precision

Intra-Assay: CV<10%, Inter-Assay: CV<12%

Detection Method

Competitive Inhibition

Assay Time

2 hours

Application

ELISA

Assay Type

Competitive Inhibition

Shipping Condition

Ice packs

Storage

Short term: 4°C; Long term: see manual.

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Expiry Date

8 months

Specificity

This assay has high sensitivity and excellent specificity for detection of Kanamycin (KNM). No significant cross-reactivity or interference between Kanamycin (KNM) and analogues was observed.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Galectin 12 (GAL12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Galectin 12 (GAL12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Galectin 12 (GAL12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Galectin 12 (GAL12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay procedure summary

1. Prepare all reagents, samples and standards; 2. Add 50µl standard or sample to each well.And then add 50µl prepared Detection Reagent A immediately.Shake and mix. Incubate 1 hour at 37°C ; 3. Aspirate and wash 3 times; 4. Add 100µl prepared Detection Reagent B. Incubate 30 minutes at 37°C ; 5. Aspirate and wash 5 times; 6. Add 90µl Substrate Solution. Incubate 10-20 minutes at 37°C ; 7. Add 50µl Stop Solution. Read at 450 nm immediately.

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Restrictions

For Research Use Only. Not for use in diagnostic procedures. Not for human or animal drug or food use.

Quality Assurance

Biomatik ELISA Kits are not typically pre-assembled as finished products due to limited shelf life. Before shipping, each kit is assembled and tested to ensure that it meets specifications before shipping. Minor changes may occur to the Detection Range, Sensitivity, and Precision. All kits are tested to confirm that they fall within their defined Inter- and Intra- assay coefficient of variation. Please always refer to the hard copy manual included in the kit before experiment.

Research Area

Metabolic pathway;

艾美捷卡那霉素ELISA试剂盒中文说明书：

测试原理：

卡那霉素ELISA试剂盒采用的检测原理是夹心酶免疫法。该试剂盒中提供的微量滴定板已预先涂有半乳糖凝集素 12 (GAL12) 特异性抗体。然后将标准或样品添加到适当的微量滴定板孔中，其中含有对半乳凝素 12 (GAL12) 特异的生物素偶联抗体。接下来，将与辣根过氧化物酶 (HRP) 结合的抗生物素蛋白添加到每个微孔板孔中并孵育。加入 TMB 底物溶液后，只有含有 Galectin 12 (GAL12)、生物素偶联抗体和酶偶联 Avidin 的孔才会出现颜色变化。通过添加硫酸溶液终止酶-底物反应，并在 450nm ± 10nm 的波长下用分光光度计测量颜色变化。

检测程序总结：

1. 准备好所有试剂、样品和标准品；2. 每孔加入50µl 标准品或样品，然后立即加入50µl 制备的检测试剂A。摇匀。37°C 孵育 1 小时；3.吸出水洗3次；4. 加入 100µl 制备的检测试剂 B，37°C 孵育 30 分钟；5.吸出并洗涤5次；6. 加入 90µl 底物溶液。在 37°C 下孵育 10-20 分钟；7. 加入 50µl 终止液。立即在 450 nm 处读取。

稳定：

试剂盒的稳定性由活性损失率决定。在适当的储存条件下，本试剂盒在有效期内的丢失率小于5%。为了尽量减少对性能、操作程序和实验室条件的额外影响，特别是室温、空气湿度，应严格控制培养箱温度。还强烈建议从头到尾由同一操作员执行整个测定。

限制：

仅供研究使用。不用于诊断过程。不用于人类或动物药物或食品。

质量保证：

由于保质期有限，Biomatik ELISA 试剂盒通常不会作为成品进行预组装。发货前，每个套件都经过组装和测试，以确保在发货前符合规格。检测范围、灵敏度和精度可能会发生细微变化。所有试剂盒都经过测试，以确认它们在定义的测定间和测定内变异系数范围内。实验前请务必参考套件中包含的硬拷贝手册。

研究领域：

代谢途径；

艾美捷卡那霉素ELISA试剂盒检测对卡那霉素（KNM）的检测具有较高的灵敏度和特异性。卡那霉素（KNM）和类似物之间未观察到明显的交叉反应或干扰。

来源：https://www.amyjet.com/products/EKU12254.shtml