中英文说明丨艾美捷 pVectOZ转染质粒

pVectOZ Transfection Plasmids are engineered in an optimized backbone and are ideal for all transfections. OZ Biosciences proposes five expressions vectors encoding for the most popular reporter genes (CAT, GFP, LacZ, Luciferase, SEAP)

pVectOZ- encoding genes:

Chloramphenicol Acetyltransferase (CAT) – pVectOZ-CAT

Green Fluorescent Protein (GFP) – pVectOZ-GFP

beta-Galactosidase (Lac Z) – pVectOZ-LACZ

Luciferase (Luc) – pVectOZ-LUC

Secreted Alcaline Phosphatase (SEAP) – pVectOZ-SEAP

The transgene expression level depends mainly on the promoter, enhancer, terminator and plasmid backbone. The pVectOZ expression cassette was designed to express transgene product in many mammalian cells and tissues. This vector has been modified to eliminate sequences affecting transgene expression levels while optimizing those critical for the high levels of expression. The final expression cassette accommodates the high levels of transgene expression in mammalian cells as well as high yield of plasmid production in Escherichia coli. The resulting plasmid is the ideal vector for expression in vitro and in vivo.

pVectOZ main features:

Highest levels of transgene expression in mammalian cells and tissues

Suitable for all transfection applications (in vivo & in vitro)

High copy number production in Escherichia coli

LPS-endotoxin free, supercoiled and highly purified: transfection grade approved

Sizes: 25µg or 100µg

Storage: -20°C

Shipping Conditions: Room Temperature

艾美捷OZ Biosciences pVectOZ转染质粒#PL00010在优化的主链中设计，是所有转染的理想选择。OZ Biosciences提出了五种表达载体，编码最流行的报告基因（CAT，GFP，LacZ，Luciferase，SEAP）

pVectOZ-编码基因：

氯霉素乙酰转移酶 （CAT） – pVectOZ-CAT

绿色荧光蛋白 （GFP） – pVectOZ-GFP

β-半乳糖苷酶 （Lac Z） – pVectOZ-LACZ

荧光素酶 （Luc） – pVectOZ-LUC

分泌的碱性磷酸酶 （SEAP） – pVectOZ-SEAP

转基因表达水平主要取决于启动子、增强子、终止子和质粒骨架。pVectOZ表达盒设计用于在许多哺乳动物细胞和组织中表达转基因产物。该载体已经过修改，以消除影响转基因表达水平的序列，同时优化那些对高表达水平至关重要的序列。最终的表达盒适应哺乳动物细胞中高水平的转基因表达以及大肠杆菌中质粒产生的高产量。所得质粒是体外和体内表达的理想载体。

OZ Biosciences pVectOZ转染质粒主要特点：

哺乳动物细胞和组织中最高水平的转基因表达

适用于所有转染应用（体内和体外）

大肠杆菌拷贝数高

不含LPS-内毒素，超螺旋和高度纯化：转染级认证

规格： 25μg 或 100μg

储存： -20°C

运输条件：室温

OZ Biosciences pVectOZ转染质粒的结果：

图1：COS-7细胞中的荧光素酶剂量反应。COS-7使用多量的pVectOZ-LUC和DreamFect™转染试剂进行转染。转染后48h测定荧光素酶活性。

图2：MCF-7细胞中的X-Gal表达。MCF-7使用DreamFect Gold™转染试剂转染0.5μg pVectOZ-LacZ。使用X-Gal染色试剂盒（GX48）在转染后10003h监测β-半乳糖苷酶活性。

来源：https://www.amyjet.com/products/OZB-PI10100.shtml