中英文介绍丨艾美捷POLYMAG CRISPR 200 转染试剂

PolyMag CRISPR kit is the only magnet assisted transfection reagent optimized to deliver high level of plasmid DNA and/or mRNA expressing Cas9 and guide RNA (gRNA). This reagent is based on our proprietary Magnetofection™ technology. The nanoparticles are composed of iron oxide magnetic core coated with cationic molecules. For your gene editing experiments, this reagent provides high transfection efficiency without toxicity.

Benefits:

•High efficiency delivery of CRISPR/Cas9 system even on primary and hard to transfect cells

•Ideal for co-transfection of pDNA/pDNA, pDNA/gRNA, pDNA/mRNA

•Optimized for large plasmid DNA containing Cas9, and guide RNA

•Low cell toxicity - enable to start your experiment with less cell

艾美捷POLYMAG CRISPR 200 转染试剂#PNC40200是经过优化的磁铁辅助转染试剂，可提供表达 Cas9 和向导 RNA （gRNA） 的高水平质粒 DNA 和/或 mRNA。该试剂基于我们专有的磁感应™技术。纳米颗粒由涂有阳离子分子的氧化铁磁芯组成。对于您的基因编辑实验，该试剂具有高转染效率，无毒性。

POLYMAG CRISPR 200 转染试剂特点：

• 即使在原代细胞和难以转染的细胞上也能高效递送 CRISPR/Cas9 系统• 非常适合共转染 pDNA/pDNA、pDNA/gRNA、pDNA/mRNA

• 针对含有 Cas9 的大质粒 DNA 进行了优化，并引导 RNA

• 低细胞毒性 - 能够以更少的细胞开始实验

POLYMAG CRISPR 200 转染试剂结果示例：

图：PolyMag CRISPR共转染效率用于CRISPR-Cas9实验。将NIH-3t3、HeLa和MDCK细胞接种在完全培养基中的24孔板中，转染时最佳密度为50-70%汇合。用GFP-Cas9质粒和RFP pDNA转染细胞，然后在37oC下孵育过夜。转染后24h荧光显微镜监测GFP和RFP表达。

来源：https://www.amyjet.com/products/OZB-PNC40200.shtml