中英文说明书丨ICL人Alpha1-抗胰凝乳蛋白酶ELISA试剂盒

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人Alpha1-抗胰凝乳蛋白酶ELISA试剂盒英文说明书：

The principle of the double antibody sandwich Human Alpha 1-Antichymotrypsin (SERPINA3) ELISA is represented in Figure 1. In this assay the Alpha 1-Antichymotrypsin present in samples reacts with the anti-Human Alpha 1-Antichymotrypsin antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-Alpha 1-Antichymotrypsin antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form. complexes with the previously bound Alpha 1-Antichymotrypsin. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of Alpha 1-Antichymotrypsin in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of Alpha 1-Antichymotrypsin in the test sample. The quantity of Alpha 1-Antichymotrypsin in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.

Details：

More Information

Antibody Type ELISA

Reactivity Human

Host Goat

Specificity/Target Alpha 1-Antichymotrypsin

Size 1.0

Detection Range 6.25 ng/ml - 400 ng/ml

Sensitivity 2.518 ng/ml

Assay Time 80 min.

Sample Type Plasma, Serum

Storage 2-8C

Product Citations:

Wågsäter D, et al. Serine protease inhibitor A3 in atherosclerosis and aneurysm disease INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 30: 288-294, 2012

Manal L NIAZI, M.D. and MAGED ABD EL-NASEER, M.D Serum Inflammatory Markers in Dementia Disorders Med. J. Cairo Univ., Vol. 77, No. 4, June: 337-346, 2009

人Alpha1-抗胰凝乳蛋白酶ELISA试剂盒中文说明书：

人α1-抗胰蛋白酶（SERPINA3）ELISA的双重抗体夹心原理如图1所示。在这种测定中，样本中存在的α1-抗胰蛋白酶与已吸附到聚苯乙烯微孔板表面的抗人类α1-抗胰蛋白酶抗体发生反应。通过洗涤去除未结合的蛋白后，加入与辣根过氧化物酶（HRP）结合的抗α1-抗胰蛋白酶抗体。这些带有酶标的抗体与之前结合的α1-抗胰蛋白酶形成复合物。在另一个洗涤步骤之后，通过添加色原性底物3,3',5,5'-四甲基联苯胺（TMB）来测定免疫吸附物上结合的酶。结合酶的数量与被测试样本中α1-抗胰蛋白酶的浓度直接相关；因此，450纳米处的吸光度是测试样本中α1-抗胰蛋白酶浓度的衡量标准。测试样本中α1-抗胰蛋白酶的数量可以从标准曲线上插值得出，并根据样本稀释进行校正。