艾美捷LDN Labor Diagnostika Nord-DHEA-S ELISA定量方案

LDN Labor Diagnostika Nord-DHEA-S ELISA：用于人血清中硫酸脱氢表雄酮（DHEA-S）直接定量测定的酶免疫测定法。

艾美捷LDN Labor Diagnostika Nord-DHEA-S ELISA（AA E-1100R）：

名称：DHEA-S ELISA

描述：用于人血清中DHEA-S直接定量测定的酶免疫测定法。

设计：ELISA

样本体积：25微升血清

总检测时间：60分钟

标准范围：0/0.005 – 10微克/毫升

灵敏度：0.005微克/毫升

储存条件：2 – 8°C

试剂盒：96次测定

CE认证：是

LDN Labor Diagnostika Nord-DHEA-S ELISA检测原理：

在未标记的抗原（存在于标准品、对照品和样品中）和无酶标记抗原（缀合物）之间发生竞争，争夺微孔板上有限数量的抗体结合位点。洗涤和倾析程序去除未结合的材料。在洗涤步骤之后，酶底物被除去。通过加入终止溶液终止酶促反应。吸光度通过微量滴定板读数器进行测量。所形成的颜色的强度与样品中DHEA-S的浓度成反比。一组标准用于绘制标准曲线，从该曲线可以直接读取DHEA-S样品和对照的量。

LDN Labor Diagnostika Nord-DHEA-S ELISA参考文献：

1. Chasalow FI, Blethen SL, Bradlow HL. Dehydroepiandrosterone Sulfate (DHEAS) and DHEAS-likeCompounds in Fibrocystic Disease of the Breast. Steroids. 1988; 52(3):205–15.

2. Chasalow FI, et al. Serum Levels of Dehydroepiandrosterone Sulfate as Determined by Commercial Kitsand Reagents. Steroids. 1989;54(4):373–81.

3. de Peretti E, Forest MG. Pattern of Plasma Dehydroepiandrosterone Sulfate Levels in Humans from Birthto Adulthood: Evidence for Testicular Production. J Clin Endocrinol Metab. 1978; 47(3):572–77.

4. Holtzclaw WD, Gordon GB. Measurement of Serum Levels of Dehydroepiandrosterone Sulfate: AComparison of Radioimmunoassay and Enzymatic Analysis. Steroids. 1989; 54(4): 355–71.

5. Koritnik DR, et al. A Radioimmunoassay for Dehydroepiandrosterone Sulfate in the Circulation of RhesusMonkeys. Steroids. 1983; 42(6):653–67.

6. Orentreich N, et al. Age Changes and Sex Differences in Serum Dehydroepiandrosterone SulfateConcentrations Throughout Adulthood. J Clin Endocrol Metab. 1984; 59(3):551–55.

7. Smith MR, et al. A Radioimmunoassay for the Estimationof Serum Dehydroepiandrosterone Sulphate inNormaland Pathological Sera. Clin Chim Acta. 1975; 65(1): 5–13.

8. Check JH, et al. Falsely Elevated Steroidal Assay Levels Related to Heterophile Antibodies Against VariousAnimal Species. Gynecol Obstet Invest. 1995; 40(2):139–40.

LDN Labor Diagnostika Nord GmbH & Co. KG（简称LDN）旨在开发、制造和向临床和研究实验室市场提供专业的体外诊断测试系统和创新技术。艾美捷科技是LDN Labor Diagnostika Nord的中国代理商，为科研工作者提供优质的产品与服务。

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