抗凋亡基因（CED-9）提高植株对盐胁迫和氧化应激的耐受性

关键词：离子流(Ion flux), 膜转运(Membrane transport), 凋亡(Apoptosis), 盐胁迫(Salinity), 氧化应激(Oxidative stress)

参考文献：Sergey Shabala, et al. Planta, 2007, 227:189 -197

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Abstract

Apoptosis, one form. of programmed cell death (PCD), plays an important role in mediating plant adaptive responses to the environment. Recent studies suggest that expression of animal anti-apoptotic genes in transgenic plants may signiWcantly improve a plant’s ability to tolerate a variety of biotic and abiotic stresses. The underlying cellular mechanisms of this process remain unexplored. In this study, we investigated speciWc ion Xux “signatures” in Nicotiana benthamiana plants transiently expressing CED-9 anti-apoptotic gene and undergoing salt- and oxidative stresses. Using a range of electrophysiological techniques, we show that expression of CED-9 increased plant salt and oxidative stress tolerance by altering K⁺ and H⁺ Xux patterns across the plasma membrane. Our data shows that PVX/CED-9 plants are capable of preventing stress-induced K⁺ eZux from mesophyll cells, so maintaining intracellular K⁺ homeostasis. We attribute these eVects to the ability of CED-9 to control at least two types of K⁺-permeable channels; outward-rectifying depolarization-activating K+ channels (KOR) and non-selective cation channels (NSCC). A possible scenario linking CED-9 expression and ionic relations in plant cell is suggested. To the best of our knowledge, this study is the Wrst to link “ion Xux signatures” and mechanisms involved in regulation of PCD in plants.