现在做蛋白组学最牛的质谱是……

现在做蛋白组学最牛的质谱是什么？
几年前的答案是：Q-TOF或者FT
现在呢？
当然是Orbitrap了！

Thermo 的Orbitrap在国内好像有几台了，近期可能还要增加，呵呵，很贵的说。
发篇Anal. Chem.最新的封面文章，PDF的全文在附件里：）
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Anal. Chem., 79 (10), 3525 -3534, 2007. 10.1021/ac070020k S0003-2700(07)00020-0
Web Release Date: April 19, 2007

Copyright © 2007 American Chemical Society
Implementation of Electron-Transfer Dissociation on a Hybrid Linear Ion Trap-Orbitrap Mass Spectrometer

Graeme C. McAlister, Doug Phanstiel, David M. Good, W. Travis Berggren, and Joshua J. Coon*

Departments of Chemistry and Biomolecular Chemistry, University of Wisconsin, Madison, Wisconsin 53706, and WiCell Research Institute, Madison, Wisconsin 53706

Received for review January 3, 2007. Accepted March 20, 2007.

Abstract:

We describe the adaptation of a hybrid quadrupole linear ion trap-orbitrap mass spectrometer to accommodate electron-transfer ion/ion reactions (ETD) for peptide and protein characterization. The method utilizes pulsed, dual electrospray ion sources and requires minimal instrument modification. Switching between cation and reagent anion injection schemes is automated and accomplished within a few hundred milliseconds. Ion/ion reactions are conducted within the linear ion trap, after which the c- and z-type product ions are passed to the orbitrap for high-resolution m/z analysis. With this arrangement, mass accuracies are typically measured to within 2 ppm at a resolving power of ~60 000. Using large peptides and intact proteins, we demonstrate such capabilities will accelerate our ability to interrogate high-mass species. To illustrate compatibility with automated data-dependent analysis and subsequent data processing, we couple the technique with an online chromatographic separation of a yeast whole-cell lysate followed by peptide identification using ProSight PC. Fairly long pulsing times and relatively low ET efficiency, as compared to conventional ETD instrumentation, are the main drawbacks of this approach. Still, our results suggest that the implementation of ETD on sensitive, high-resolution, and high-mass accuracy hybrid instrumentation, such as the orbitrap, will substantially propel the emergent fields of middle- and top-down proteomics.

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