阿拉伯聚糖检测试剂盒 产品货号:K-ARAB
阿拉伯聚糖检测试剂盒 产品货号:K-ARAB
产品货号:K-ARAB
品牌:Megazyme
产品品名:阿拉伯聚糖检测试剂盒
英文品名:Arabinan Assay Kit
规格型号:100 assays per kit
产品品名:阿拉伯聚糖检测试剂盒
英文品名:Arabinan Assay Kit
规格型号:100 assays per kit
For the measurement of arabinan in fruit juice concentrates.
INTRODUCTION:
In the processing of apples and pears, the yield of juice can be dramatically improved by using enzymes to degrade the pulp polysaccharides, together with more exhaustive extraction of the pulp with diffusion equipment. Of course, these processes also significantly increase the amount of partially degraded polysaccharide which is solubilised. This polysaccharide material may be soluble as extracted, but subsequent changes in temperature and pH conditions can directly lead to precipitation (or crystallisation) or to chemical modification followed by precipitation. Such a problem can be experienced in the production of clear apple or pear juice, in which case, an arabinan haze material is produced. This material was shown to be microcrystalline 1,5-α-L-arabinan. Arabinans, as present in cell-wall pectic-substances, have been shown to consist of a main chain of 1,5-α-linked L-arabinofuranosyl residues to which other L-arabinofuranosyl residues are linked 1,3-α and 1,2-α in either a comb-like or a ramified arrangement. It is generally accepted that the best solution to this “arabinan haze” problem is to use pectinase enzyme preparations containing high levels of both α-L-arabinofuranosidase and endo-1,5-α-Larabinanase. The combined action of these two enzymes reduces arabinan to arabinose and lower degree of polymerisation, arabinooligosaccharides.
KITS:
Kits suitable for performing 100 assays are available from Megazyme.The kits contain the full assay method plus:
Bottle 1: Buffer (25 mL, pH 8.6) plus sodium azide (0.02 %w/v) as a preservative (Buffer A).Stable for > 2 years at 4°C.
Bottle 2: NAD .Stable for > 5 years at -20°C.
Bottle 3: α-L-Arabinofuranosidase plus endo-arabinanasesuspension (2.2 mL).Stable for > 4 years at 4°C.
Bottle 4: β-Galactose dehydrogenase plus galactose mutarotasesuspension, 2.2 mL. Stable for > 2 years at 4°C.
Bottle 5: L-Arabinose standard solution (5 mL, 0.5 mg/mL in0.02 % sodium azide).Stable for > 4 years at 4°C.
Bottle 6: Arabinan control in mannitol: Arabinanconcentration on vial. Stable for > 5 years at 4°C.
Component 7: Pharmacia PD-10 column.
Bottle 1: Buffer (25 mL, pH 8.6) plus sodium azide (0.02 %
Bottle 2: NAD .Stable for > 5 years at -20°C.
Bottle 3: α-L-Arabinofuranosidase plus endo-arabinanase
Bottle 4: β-Galactose dehydrogenase plus galactose mutarotase
Bottle 5: L-Arabinose standard solution (5 mL, 0.5 mg/mL in
Bottle 6: Arabinan control in mannitol: Arabinan
Component 7: Pharmacia PD-10 column.
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