Promega在ASMS 2009上推出鉴定蛋白质的新途径
Promega利用ProteaseMAX表面活性剂推出了一项创新简化的胶内酶切技术。该项技术给分析人员提供了以下几点好处：
- 节省时间：整个胶内酶切过程只需一个小时的时间
- 省去了萃取步骤：蛋白质分解和肽段回收一步完成。
- 更多的数据：该分解技术保持了原有肽段的信息。
除了ProteaseMAX 表面活性剂意外，Promega还推出了以下几款新产品：
- 固定化胰蛋白酶
- 胰凝乳蛋白酶测序级
- Lys-C测序级
固定化胰蛋白酶提高了纯化蛋白质的分离速度。蛋白酶切过程只需短短的30分钟，可适用于多种浓度的蛋白质。胰凝乳蛋白酶和Lys-C既能单独使用也可以结合其它蛋白酶，通过肽质量指纹图谱或MS/MS谱图匹配的方法来识别蛋白质分解产生的肽谱，从而进行蛋白质鉴定。该两项蛋白酶都适用于在溶液或胶内催化蛋白质分解。
参考英文原文
Identify proteins you could not identify before using new products from Promega
Promega Corp.
Promega introduces an innovative and simplified in-gel digestion procedure utilizing ProteaseMAX Surfactant. This new procedure offers the researcher multiple benefits:
• Save time: Digestions complete in only one hour.
• Eliminate gel extraction steps: Protein digestion and peptide recovery is complete in a single step.
• More data: Recover longer peptides which tend to remain in the gel in conventional in-gel digestion protocols.
In addition to ProteaseMAX Surfactant, Promega now offers the following new products:
• Immobilized Trypsin
• Chymotrypsin Sequencing Grade
• Lys-C Sequencing Grade
Immobilized Trypsin offers fast digestion of purified proteins or complex mixtures. Digestions are complete in as little as 30 minutes and the protocol is easily scalable to accommodate multiple protein concentrations. Chymotrypsin and Lys-C can both be used alone or in combination with other proteases to produce protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. Both proteases are suitable for digestion reactions in-solution or in-gel.
Request your free sample of Immobilized Trypsin, just visit us at ASMS booth #63 to find out how.
