Foodstuffs. Determination of fumonisin B1 and fumonisin B2 in processed maize containing foods for infants and young children. HPLC method with immunoaffinity column cleanup and fluorescence detection after precolumn derivatization
1 Scope
This Technical Specification specifies a method for the determination of fumonisin
B 1 (FB 1 ) and fumonisin B 2 (FB 2 ) in processed maize-containing foods for infants and young children by high performance
liquid chromatography (HPLC) with immunoaffinity cleanup and fluorescence detection
(FLD). This method has been validated in an interlaboratory study via the analysis
of both naturally contaminated and spiked samples ranging from 112 µg/kg to 458 µg/kg
for FB 1 +FB 2 , 89 µg/kg to 384 µg/kg for FB 1 and 22 µg/kg to 74 µg/kg for FB 2 .
For further information on the validation see Clause 8 and Annex B.