中英文说明丨艾美捷GMP IL-1β#C01002

Interleukin-1 β （IL-1 β） Also known as endogenous mediators of white blood cells, monocytes, lymphocyte activating factors, etc., it is a cytokine protein encoded by the IL1B gene in humans. Interleukin-1 (IL-1) has two genes: IL-1 α And IL-1 β (This gene). IL-1 β Precursor cytoplasmic caspase 1 (interleukin-1 β Transformase cleavage to form. mature IL-1 β。

Croyez Aimejie GMP IL-1 β# C01002 Details:

Source labeled with polyhistidine

Escherichia coli

No animal reagents and laboratories

According to GMP guidelines

Manufacturing and testing of endotoxin levels

Every 0 through the LAL method μ G protein<1.1 EU.

activity

Measured by its ability to induce IL-29 secretion in HT8 cells. The effect of Education Department 50 is<2.0 nanograms per milliliter.

Recombinant IL-1 β The specific activity is about>3 × 107IU/mg.

purity

>98% was determined by SDS-PAGE. Purified by Ni NTA chromatography.

formula

Freeze dry the protein from a solution containing 1X PBS and pH 8.0.

Re dissolution is recommended in sterile H

Freeze dried protein 2O to a concentration not less than 100 μ G/mL and incubate the stock solution for at least 20 minutes to ensure sufficient redissolution.

Freeze dried protein should be stored

At -20 ° C. This product can be stably stored for one year upon receipt, and processed and stored according to the instructions. After re dissolution, the protein sample should be stored at -20 ° C or -80 ° C. Avoid repeated freeze-thaw cycles.

be careful

Please use it within one month after protein re dissolution.

Croyez艾美捷GMP IL-1β#C01002详细信息：

活性

通过其在HT8细胞中诱导IL-29分泌的能力来衡量。教育署50这种效果是<2.0纳克/毫升。

重组人IL-1β的比活性约为>3×107IU/mg.

纯度

>98% 由 SDS-PAGE 测定。通过Ni-NTA色谱法纯化。

配方

将蛋白质从含有1X PBS，pH 8.0的溶液冻干。

复溶建议在无菌H中复溶

冻干蛋白2O至浓度不低于100μg/ mL，并将储备溶液孵育至少20分钟以确保充分重新溶解。

储存冻干蛋白应储存

在-20°C。 本产品在收到后可稳定保存一年，并按照说明进行处理和储存。复溶后，蛋白质等分试样应储存在-20°C或-80°C。 避免反复冻融循环。

注意

请在蛋白质复溶后一个月内使用完毕。

GMP IL-1β相关研究：

→ IL-1β（白细胞介素-1β），人【C01002】

→ Croyez GMP IL-4（白细胞介素-4），人【C01006-GMP】

→ IL-4（白细胞介素-4），人【C01006】

→ Croyez GMP TGFβ1（转化生长因子β1），人【C01088-GMP】

→ 转化生长因子β1，人类【C01088】

→ IL-9（白细胞介素-9），人【C01011】

→ Croyez GMP IL-21（白细胞介素-21），人【C01026-GMP】

→ IL-21（白细胞介素-21），人【C01026】

→ Croyez GMP干扰素-γ（干扰素-γ），人【C01080-GMP】

→ 干扰素-γ（干扰素γ），人类【C01080】

来源：https://www.amyjet.com/brand/gmp-il-1-beta.shtml