Enzyme Immobilization on Reactive Polymer Films
Immobilized enzymes are currently used in many bioanalytical and biomedical applications. This protocol describes the use of thin films of maleic anhydride copolymers to covalently attach enzymes directly to solid supports at defined concentrations. The concentration and activity of the surface-bound enzymes can be tuned over a wide range by adjusting the concentration of enzyme used for immobilization and the physicochemical properties of the polymer platform, as demonstrated here for the proteolytic enzyme Subtilisin A. The versatile method presented allows for the immobilization of biomolecules containing primary amino groups to a broad variety of solid carriers, ranging from silicon oxide surfaces to standard polystyrene well plates and metallic surfaces. The approach can be used to investigate the effects of immobilized enzymes on cell adhesion, and on the catalysis of specific reactions.
- 热休克蛋白
- 钙磷代谢及微量元素
- Visualizing the needle in the haystack: In situ hybridization with fluorescent dendrimers
- Preparation and Assay of Phage Lambda
- 键合相色谱法
- 葡萄酒
- 条纹苹果
- 蛋白复性(五)-蛋白质复性
- A Sensitive Assay for the Enzymatic Activity of GTP Cyclohydrolase I
- Live Cell Imaging of Chemotactic Dendritic Cell Migration in Explanted Mouse Ear Preparations