Nonradioactive Labeling of DNA
Many important advances in molecular biology would not have been possible wlthout the use of radioisotopes It is relatively simple to substitute a radtoacttve isotope into a nucleotide to produce a molecule that has the same biological properties as the unlabeled molecule (see Chapter 6) These molecules are mcorporated into DNA sequences by a variety of protocols Unfortunately, with the high efficiency and easy incorporation of radionuchdes comes a Pandora’s Box of difficulties The short half lives of the most commonly used nucleotides (32P,33P, 35S) necessitate that the material be freshly labeled for optimal efficiency This alone makes it difficult for scientists m developing nations to use isotopic techniques. The ability of radiation to penetrate human tissue and cause damage requires that all work be done from behind shielding material Personnel exposure to radiation must be monitored on a regular basis A problem that is now looming large for the use of radiation in the molecular-biology laboratory is the lack of disposal sites for radioactive waste. Many regulatory agencies will not issue licenses to work with radlatton if no disposal site is available
- Using RNA Interference in Schistosoma mansoni
- Solid-Phase Guanidinylation of Peptidyl Amines Compatible with Standard Fmoc-Chemistry: Formation of Monosubstituted Guanidines
- “嘌呤型”三螺旋DNA
- Free Zone Capillary Electrophoresis
- 高效毛细管电泳法在酶反应动力学研究中的应用
- 人胰高血糖素样肽1(GLP-1)酶联免疫分析(ELISA)
- 石榴(pomegranate)
- 离子交换层析(Ion Exchange Chromatography ,IEC)
- 免疫血清的制备及免疫球蛋白的提取
- 逆转录-聚合酶链反应 (Reverse Transcription-Polymerase Chain Reaction,RT-PCR