Redox Equivalents and Mitochondrial Bioenergetics
关键词: redox equivalents mitochondrial来源: 互联网
Mitochondrial energy metabolism depends upon high-flux and low-flux electron transfer pathways. The former provide the energy to support chemiosmotic coupling for oxidative phosphorylation. The latter provide mechanisms for signaling and control of mitochondrial functions. Few practical methods are available to measure rates of individual mitochondrial electron transfer reactions; however, a number of approaches are available to measure steady-state redox potentials (E h ) of donor/acceptor couples, and these can be used to gain insight into rate-controlling reactions as well as mitochondrial bioenergetics. Redox changes within the respiratory electron transfer pathway are quantified by optical spectroscopy and measurement of changes in autofluorescence. Low-flux pathways involving thiol/disulfide redox couples are measured by redox western blot and mass spectrometry-based redox proteomics. Together, the approaches provide the opportunity to develop integrated systems biology descriptions of mitochondrial redox signaling and control mechanisms.
推荐方法
- Yeast exoglycoproteins produced under NaCl-stress conditions as efficient cryoprotective agents
- Integration of Phosphodiesterase-Induced Degradation of Oligonucleotides with Capillary Polymer-Sieving Electrophoresis
- Antineoplastic Drugs
- 稻恶苗病
- Specimen Preparation for Thin-Section Electron Microscopy Utilizing Microwave-Assisted Rapid Processing in a Veterinary Diagnost
- Electron Paramagnetic Resonance Spectroscopy
- 藻类的超氧化物岐化酶(SOD)酶联免疫分析(ELISA)
- 我国将投2万亿发展可再生能源
- 桑椹酱
- 粘性末端连接