Integration of SPR Biosensors with Mass Spectrometry (SPR-MS)
The combination of surface plasmon resonance (SPR) and mass spectrometry (MS) creates a comprehensive protein investigation approach wherein SPR is employed for protein quantification and MS is utilized to structurally characterize the proteins. In such, MS utterly complements the SPR detection and reveals intrinsic protein structural modifications that go unregistered via the SPR detection. Protein complexes and non-specific binding can also be delineated via the SPR-MS approach. Described here are the protocols and know-how for successful and reproducible integration of SPR and MS. The individual steps of the entire SPR-MS process are illustrated via an example showing analysis of myoglobin from human plasma.
- Affinity Capillary Electrophoresis to Examine Receptor-Ligand Interactions
- Molecular Cloning in Bacteriophage Lambda and in Cosmids
- Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Complexes
- Liposome Preparation
- 急性时相反应蛋白(acutephasereactants,APR)
- 三磷酸腺苷(ATP)
- 多味葡萄
- Tris–盐酸缓冲液(0.05M,25℃)的配制
- 甘油三酯的分解代谢
- 基因敲除/基因打靶的相关资料整理