Enantioseparation by Chiral Ligand-Exchange Capillary Electrophoresis
Chiral ligand-exchange capillary electrophoresis is a relatively cheap and flexible format of capillary electrophoresis. It separates chiral analytes with a metal complex chiral selector that is composed of a metal ion and either homogeneous or heterogeneous chiral ligands (in respect of analytes). The analytes to be separated are required to possess the ability to coordinate with the metal ion, commonly containing N and/or O atoms, for example, amino acids or amino alcohols. In present chapter we take the separation of dansyl dl-amino acids as a key example to guide through the setup of a chiral ligand-exchange capillary electrophoresis enantioseparation protocol. The critical working conditions emphasized include the selection of metal ion, pH window, the ligand-to-metal ratio and/or the concentrations of the complex and electrolytes. In addition, trouble-shooting advice is given such as the way to overcome baseline drifting.
- Lipase Activation and Stabilization in Room-Temperature Ionic Liquids
- 人降钙素基因相关肽(CGRP)酶联免疫分析(ELISA)
- 天蚕(Japanese tussah silkworm)
- 影响纸层析分离中迁移率因素
- 基因工程菌—大肠杆菌(E.coli)
- Lectin Inhibition of Bacterial Adhesion to Animal Cells
- Synthesis of Lipidated Peptides
- 醛基
- 生物质能开启新能源时代之门
- Measurements of Ca2+ Concentration with Recombinant Targeted Luminescent Probes